lncRNA involvement in hepatocellular carcinoma metastasis and prognosis

Abbastabar, Maryam; Sarfi, Mohammad; Golestani, Abolfazl; Khalili, Ehsan

doi:10.17179/excli2018-1541

Cited by 77 publications

(20 citation statements)

References 113 publications

(150 reference statements)

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“…It has been shown that lncRNA, a potential biomarker, is important in tumor growth and metastasis ( 12 ). Therefore, it is feasible to study the expression of lncRNAs to evaluate the prognosis of HCC ( 13 ). Equally important is N6-methyladenosine (m6A), a methylated modification that occurs in RNA and is involved in the cleavage, transportation, stability, and degradation of noncoding RNA ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of m6A- and ferroptosis-related lncRNA signature for predicting immune efficacy in hepatocellular carcinoma

et al. 2022

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BackgroundN6-methyladenosine (m6A) methylation and ferroptosis assist long noncoding RNAs (lncRNAs) in promoting immune escape in hepatocellular carcinoma (HCC). However, the predictive value of m6A- and ferroptosis-related lncRNAs (mfrlncRNAs) in terms of immune efficacy remains unknown.MethodA total of 365 HCC patients with complete data from The Cancer Genome Atlas (TCGA) database were used as the training cohort, and half of them were randomly selected as the validation cohort. A total of 161 HCC patients from the International Cancer Genome Consortium (ICGC) database were used as external validation (ICGC cohort).ResultsWe first identified a group of specific lncRNAs associated with both m6A regulators and ferroptosis-related genes and then constructed prognosis-related mfrlncRNA pairs. Based on this, the mfrlncRNA signature was constructed using the least absolute shrinkage and selection operator (LASSO) analysis and Cox regression. Notably, the risk score of patients was proven to be an independent prognostic factor and was better than the TNM stage and tumor grade. Moreover, patients with high-risk scores had lower survival rates, higher infiltration of immunosuppressive cells (macrophages and Tregs), lower infiltration of cytotoxic immune cells (natural killer cells), poorer immune efficacy (both immunophenoscore and score of tumor immune dysfunction and exclusion), higher IC50, and enrichment of the induced Treg pathway, which confirmed that the mfrlncRNA signature contributed to survival prediction and risk stratification of patients with HCC.ConclusionsThe mfrlncRNA signature, which has great prognostic value, provides new clues for identifying “cold” and “hot” tumors and might have crucial implications for individualized therapy to improve the survival rate of patients with HCC.

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Section: Introductionmentioning

confidence: 99%

Identification of m6A- and ferroptosis-related lncRNA signature for predicting immune efficacy in hepatocellular carcinoma

et al. 2022

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“…Previously, it was studied that lncRNA HOTAIR was associated with metastasis, differentiation, and early recurrence. Furthermore, knockdown of HOTAIR lncRNA diminishes cell proliferation and is associated with decreased levels of MMP-9 and vascular endothelial growth factor protein, which are crucial for cell motility and metastasis [ 33 ]. According to its asymmetric carbon atom C20, Ginsenoside Rg3 can be divided into the R type and S type.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside-Rg3 inhibits the proliferation and invasion of hepatoma carcinoma cells via regulating long non-coding RNA HOX antisense intergenic

Wang

et al. 2021

Bioengineered

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Ginsenoside Rg3, a natural compound, has been reported to function as an anticancer agent for hepatoma carcinoma, while the mechanisms underlying the anticancer effects are not clear. Therefore, the objective of our study was to explore the impact of RG3 on cell migration and invasion by regulating the lncRNA HOX antisense intergenic (HOTAIR) expression involving PI3K/ AKT signaling pathway. qRT-PCR was utilized to measure the mRNA expression of HOTAIR. Furthermore, HOTAIR overexpression plasmids were transfected to SMMC-7721 and SK-Hep-1 cells. Additionally, MTT assay was used to evaluate the proliferation of transfected cells. The scratch and transwell assays were used to determine the migration and invasion ability of the cell. The protein levels were determined with Western blot. lncRNA HOTAIR was overexpressed in SMMC-7721 and SK-Hep-1 cells. Ginsenoside-Rg3 reduced the level of lncRNA HOTAIR. Overexpressed lncRNA HOTAIR offset ginsenoside-Rg3 inhibited proliferation, migration and invasion of HCC cells. Furthermore, ginsenoside-Rg3 decreased the expression of p-AKT, p-PI3K, matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9), which was reversed after the treatment of HOTAIR. LncRNA HOTAIR was overexpressed in SMMC-7721 cells. Ginsenoside-Rg3 could reduce the expression of lncRNA HOTAIR, resulting in the inhibited cell proliferation, migration and invasion. Furthermore, ginsenoside-Rg3 inhibited cell proliferation and invasion ability through the PI3k/AKT pathway. Thus, ginsenoside-Rg3 might be a potential and effective treatment for HCC.

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“…Recent studies had shown that lncRNA played an important role in the pathophysiology of cancer, such as epigenetic regulation, cell cycle regulation, and cell regulation (Berretta and Morillon, 2009). It has been identified that the dysregulation of lncRNA is closely associated with tumorigenesis, metastasis, prognosis, and diagnosis in HCC (Abbastabar et al, 2018). For instance, overexpression of MALAT-1 could promote the proliferation, migration, and invasion of liver cancer cells by inhibiting the expression of mir-146-5p, thus leading to the recurrence and metastasis of hepatocellular carcinoma (Lai et al, 2012;Li et al, 2017).In addition, Wang et al demonstrated that UCA1 might act as an endogenous sponge by repressing the expression of FGFR1 and activating an FGFR1/ERK signaling pathway in HCC (Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Classification of Hepatocellular Carcinoma Based on N6-Methylandenosine–Related lncRNAs Profiling

Yin

Zhou

Gao

et al. 2022

Front. Mol. Biosci.

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HCC is one of the most common types of malignancies worldwide and the fourth-leading cause of cancer deaths. Thus, there is an urgent need to search for novel targeted therapies in HCC. 186 m6a-related lncRNAs were screened for subsequent analysis. Two distinct m6A modification clusters were identified to be associated with the overall prognosis in TCGA-LIHC based on the m6A-related lncRNAs profiling, followed by univariate Cox regression analysis. In addition, four m6A-related lncRNAs prognostic signatures were developed and validated that could predict the OS of HCC patients, followed by univariate Cox regression, LASSO regression, and multivariate Cox regression analysis. Moreover, four m6A-related lncRNAs were identified to be related to HCC prognosis. ESTIMATE was used to evaluate the stromal score, immune score, ESTIMATE score, and tumor purity of each HCC sample. ssGSEA was performed to identify the enrichment levels of 29 immune signatures in each sample. Finally, quantitative real-time polymerase chain reaction shown that KDM4A-AS1, BACE1-AS, and NRAV expressions were upregulated in HCC patients. We proved that our m6A-related lncRNAs signature had powerful and robust ability for predicting OS of different HCC subgroups.

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lncRNA involvement in hepatocellular carcinoma metastasis and prognosis

Cited by 77 publications

References 113 publications

Identification of m6A- and ferroptosis-related lncRNA signature for predicting immune efficacy in hepatocellular carcinoma

Identification of m6A- and ferroptosis-related lncRNA signature for predicting immune efficacy in hepatocellular carcinoma

Ginsenoside-Rg3 inhibits the proliferation and invasion of hepatoma carcinoma cells via regulating long non-coding RNA HOX antisense intergenic

Classification of Hepatocellular Carcinoma Based on N6-Methylandenosine–Related lncRNAs Profiling

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