LncRNA KB-1471A8.2 Overexpression Suppresses Cell Proliferation and Migration and Antagonizes the Paclitaxel Resistance of Ovarian Cancer Cells

Zhang, Mi; Liu, Siyu; Fu, Chenyang; Wang, Xusu; Zhang, Min; Liu, Guangquan; Dai, Chencheng; Gong, Zhen; Xu, Hanzi; Fu, Ziyi; Xu, Pengfei; Xu, Juan; Jia, Xuemei

doi:10.1089/cbr.2018.2698

Cited by 16 publications

(11 citation statements)

References 30 publications

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“…In addition, the overexpression of lncRNA-FER1L4 could increase the sensitivity of ovarian cancer cells to paclitaxel by inhibiting the MAPK signaling pathway [25]. Also, lncRNA-KB-1471A8.2 was reported to be downregulated in ovarian cancer tissues and chemoresistant ovarian cancer cells and acted as tumor suppressor gene by inhibiting the expression of CDK4 [26].…”

Section: Discussionmentioning

confidence: 99%

Dys-regulation of lnc-SNHG1 and miR-216b-5p correlate with chemo-resistance and indicate poor prognosis of serous epithelial ovarian cancer

Pei

Zhao

Shuang

2020

Preprint

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Aim: This study aimed to explore whether the dysregulation of lnc-small nucleolar RNA host gene 1 (SNHG1) and miR-216b-5p correlated with chemoresistance and indicated poor prognosis of serous epithelial ovarian cancer (EOC). Methods and Results: The expression of lnc-SNHG1 was upregulated, while miR-216b-5p showed low expression in patients with chemoresistant EOC compared with patients with chemosensitive EOC. The multivariate Cox regression analysis showed that the expression of miR-216b-5p and FIGO stage were independent prognostic factors for the overall survival (OS) of patients with serous EOC. Kaplan–Meier curves revealed a significant association of the increased expression level of lnc-SNHG1 with shorter OS and disease-free survival (DFS). Patients with a low expression level of miR-216b-5p also had shorter OS and DFS. The biological functions were tested using CCK-8 assay, colony formation assay, wound healing assay, and cell apoptosis. The knockdown of SNHG1 and the overexpression of miR-216b-5p stimulated paclitaxel sensitivity in A2780/Taxol cells through inhibiting cell growth and migration and promoting apoptosis. The inhibition of miR-216b-5p could rescue the effect of lnc-SNHG1 inhibition on the sensitivity of A2780/Taxol cells to paclitaxel. Luciferase reporter assay, RNA Binding Protein Immunoprecipitation Assay (RIP), and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) indicated that lnc-SNHG1 acted as a sponge of miR-216b-5p in A2780/Taxol cells.Conclusions: This study showed that the overexpression of lnc-SNHG1 and decreased expression level of miR-216b-5p correlated with the chemoresistance of patients with serous EOC and indicated shorter OS and DFS. Lnc-SNHG1 functioned as a ceRNA with miR-216b-5p, which was critical in modulating the paclitaxel sensitivity of ovarian cancer cells.

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Section: Discussionmentioning

confidence: 99%

Dys-regulation of lnc-SNHG1 and miR-216b-5p correlate with chemo-resistance and indicate poor prognosis of serous epithelial ovarian cancer

Pei

Zhao

Shuang

2020

Preprint

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“…In addition, researchers found over-expression of lncRNA-FER1L4 could increase the sensitivity of ovarian cancer cells to paclitaxel by inhibiting MAPK signaling pathways [25]. Also, lncRNA-KB-1471A8.2 was reported to be down-regulated in ovarian cancer tissues and chemo-resistant ovarian cancer cells and could act as tumor-suppressor gene by inhibiting the expression of CDK4 [26].…”

Section: Discussionmentioning

confidence: 99%

Dys-regulation of Lnc-SNHG1 and miR-216b-5p Correlate With Chemo-resistance and Indicate Poor Prognosis of Serous Epithelial Ovarian Cancer

Pei

Zhao

Shuang

2020

Preprint

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Background: Ovarian cancer is the most lethal of gynecological cancers and the 5-year survival rate is still low and chemo-resistance is the main obstacle for the treatment of ovarian cancer. Methods and Results: In this study, the human ovarian cancer tissue microarray was applied, 63 cases of chemo-sensitive serous EOC tissues and 32 cases of chemo-resistant serous EOC tissues were selected. By applying RNA fluorescence in situ hybridization (FISH), we found expression of Lnc-SNHG1 was up-regulated while miR-216b-5p showed low expression in chemo-resistant EOC patients compared with the chemo-sensitive group, both were mainly localized in the cytoplasm of the tissue cells. Chi-square test showed high lnc-SNHG1 level was significantly correlated with tumor stage, histological grade, nodal status, metastasis and chemo-resistance except tumor size. While there was no significant association between miR-216b-5p expression and parameters including tumor stage, histological grade, nodal status, metastasis, except chemo-resistance (P=0.0001). Spearman’s correlation analysis revealed significantly negative correlations between lnc-SNHG1 and miR-216b-5p (r = -0.424, P = 0.0001). Multivariate Cox regression analysis showed the expression of miR-216b-5p (P = 0.012, RR 2.137, 95 % CI 1.109–5.339) and FIGO stage (P = 0.001, RR 3.537, 95 % CI 1.72–7.276) was independent prognostic factors for the overall survival (OS) of serous EOC patients .While the FIGO stage (P = 0.003, RR2.237, 95 % CI 1.323–3.783) was the independent prognostic factor for the disease free survival (DFS) for the serous EOC patients. Kaplan- Meier curves revealed significant association of increased expression of lnc-SNHG1 with less OS and shorter DFS, while patients with low level of miR-216b-5p indicated less OS and DFS. Conclusions: In a word, we claimed over-expression of lnc-SNHG1 and decreased expression of miR-216b-5p were correlated with chemo-resistance of serous EOC patients and indicated less OS and shorter DFS of the patients.

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“…In addition, the overexpression of lncRNA-FER1L4 could increase the sensitivity of ovarian cancer cells to paclitaxel by inhibiting the MAPK signaling pathway [ 26 ]. Also, lncRNA-KB-1471A8.2 was reported to be downregulated in ovarian cancer tissues and chemoresistant ovarian cancer cells and acted as tumor suppressor gene by inhibiting the expression of CDK4 [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of lnc-SNHG1 and miR-216b-5p correlate with chemoresistance and indicate poor prognosis of serous epithelial ovarian cancer

2020

View full text Add to dashboard Cite

Aim This study aimed to explore whether the dysregulation of lnc-small nucleolar RNA host gene 1 (SNHG1) and miR-216b-5p correlated with chemoresistance and indicated poor prognosis of serous epithelial ovarian cancer (EOC). Methods and results The expression of lnc-SNHG1 was upregulated, while miR-216b-5p showed low expression in patients with chemoresistant EOC compared with patients with chemosensitive EOC. The multivariate Cox regression analysis showed that the expression of miR-216b-5p and FIGO stage were independent prognostic factors for the overall survival (OS) of patients with serous EOC. Kaplan–Meier curves revealed a significant association of the increased expression level of lnc-SNHG1 with shorter OS and disease-free survival (DFS). Patients with a low expression level of miR-216b-5p also had shorter OS and DFS. The biological functions were tested using CCK-8 assay, colony formation assay, wound healing assay, and cell apoptosis. The knockdown of SNHG1 and the overexpression of miR-216b-5p stimulated paclitaxel sensitivity in A2780/Taxol cells through inhibiting cell growth and migration and promoting apoptosis. The inhibition of miR-216b-5p could rescue the effect of lnc-SNHG1 inhibition on the sensitivity of A2780/Taxol cells to paclitaxel. Luciferase reporter assay, RNA Binding Protein Immunoprecipitation Assay (RIP), and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) indicated that lnc-SNHG1 acted as a sponge of miR-216b-5p in A2780/Taxol cells. Conclusions This study showed that the overexpression of lnc-SNHG1 and decreased expression level of miR-216b-5p correlated with the chemoresistance of patients with serous EOC and indicated shorter OS and DFS. Lnc-SNHG1 functioned as a ceRNA with miR-216b-5p, which was critical in modulating the paclitaxel sensitivity of ovarian cancer cells.

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LncRNA KB-1471A8.2 Overexpression Suppresses Cell Proliferation and Migration and Antagonizes the Paclitaxel Resistance of Ovarian Cancer Cells

Cited by 16 publications

References 30 publications

Dys-regulation of lnc-SNHG1 and miR-216b-5p correlate with chemo-resistance and indicate poor prognosis of serous epithelial ovarian cancer

Dys-regulation of lnc-SNHG1 and miR-216b-5p correlate with chemo-resistance and indicate poor prognosis of serous epithelial ovarian cancer

Dys-regulation of Lnc-SNHG1 and miR-216b-5p Correlate With Chemo-resistance and Indicate Poor Prognosis of Serous Epithelial Ovarian Cancer

Dysregulation of lnc-SNHG1 and miR-216b-5p correlate with chemoresistance and indicate poor prognosis of serous epithelial ovarian cancer

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