lncRNA KTN1‑AS1 promotes glioma cell proliferation and invasion by negatively regulating miR‑505‑3p

Abstract

Glioblastoma (GBM) is one of the most prevalent and aggressive central nervous tumors with high mobility and mortality. The prognosis of patients with GBM is poor. It is therefore essential to explore the therapeutic strategies for the treatment of GBM. Previous studies have demonstrated that the long non-coding RNA (lncRNA) Kinectin 1-Antisense RNA 1 (KTN1-AS1) can participate in the development of several types of cancer. However, the underlying mechanism of KTN1-AS1 in GBM remains unknown. The present study aimed to determine the potential role of KTN1-AS1 in GBM. In this study, reverse transcription quantitative PCR analysis was conducted and the results demonstrated that KTN1-AS1 was upregulated in GBM tissues and cell lines compared with normal tissues and astrocytes (NHA). Furthermore, KTN1-AS1 knockdown decreased the viability and invasive ability of glioma cells in vitro and in vivo . In addition, high level of KTN1-AS1 was correlated with poor prognosis in TCGA GBM database. Furthermore, microRNA-505-3p (miR-505-3p) was a promising target of KTN1-AS1, and the suppressing effects of miR-505-3p on cell proliferation and invasive ability was reversed by downregulating KTN1-AS1. Taken together, the results from the present provided novel insights into the roles of KTN1-AS1 in GBM, and suggested that the KTN1-AS1/miR-505-3p axis may be considered as a novel therapeutic target for the treatment of patients with GBM.

Differential Expression Profile of lncRNA in Glioma Cells and the Effect of lncRNA NKX3-1 on Glioma Cells Through Fem1b/SPDEF Pathway

“…LncRNA plays a role in glioma cell proliferation, either promoting or inhibiting it. Current research indicates that a number of lncRNAs, including lncRNA PLAC2 ( 19 ), lncRNA KTN1AS1 ( 20 ), lncRNA DANCR ( 21 ), lncRNA MIR31HG ( 22 ), and others, regulate the proliferation of glioma cells via various signaling mechanisms. Overexpression of lncRNA ZEB1-AS1 has been shown in vitro to promote the proliferation, migration, and invasion of glioma cells, as well as the cell cycle, so lncRNA ZEB1-AS1 is used as a proto-oncogene in glioma tissues ( 23 ).…”

The regulatory role of antisense lncRNAs in cancer

“…Mechanistically, KTN1-AS1 recruits EP300, a histone acetyltransferase, which enriched H3K27Ac in the KTN1 promoter region, thus activating the expression of KTN1 [ 63 ]. Furthermore, KTN1-AS1 increases the viability and invasive ability of glioma cells in vitro and in vivo through the KTN1-AS1 /miR-505-3p pathway and promotes tumor growth of HCC via the miR-23c/ ERBB2IP axis [ 64 , 65 ].…”

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma

“…MicroRNAs (miRNAs), a kind of small single-stranded ncRNA, include 19–25 nt and can bind to the 3′ untranslated region (UTR) of its target mRNA to inhibit gene degradation or translation. 15 , 16 , 17 We have learned that miRNAs regulate approximately 30% of the genes in the human genome. 18 Moreover, miRNA-mediated post-transcriptional regulation requires multiple RNA-binding proteins, which are conducive to the function of miRNA in tumorigenesis.…”