LncRNA LINC-PINT Inhibits Malignant Behaviors of Laryngeal Squamous Cell Carcinoma Cells via Inhibiting ZEB1

Yang, Xianguang; Miao, Susheng; Mao, Xionghui; Cheng, Xiangdong; Sun, Ji; Pei, Rong; Song, Jia

doi:10.3389/pore.2021.584466

Cited by 11 publications

(7 citation statements)

References 40 publications

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“…ZEB1 accumulation enhanced the invasion and EMT of liver and gastric cancer cells (36,37). Importantly, ZEB1 acts as an oncogene and is closely related to EMT and prognosis in LSCC (38,39). Our study shows that ZEB1 expression was enhanced in LSCC cells, and it was inhibited by silencing HCP5 and promoted by cosilencing HCP5 and miR-216a-5p.…”

Section: Discussionmentioning

confidence: 59%

Downregulation of long noncoding RNA HCP5/miR-216a-5p/ZEB1 axis inhibits the malignant biological function of laryngeal squamous cell carcinoma cells

Zhang

Hui²,

Zhao³

et al. 2022

Front. Immunol.

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Previous studies find that long noncoding RNA human leukocyte antigen complex P5 (HCP5) is regarded as an oncogene via accelerating cancer cell growth, invasion, metastasis, vascularization, and drug resistance in renal cell carcinoma, gastric cancer, and colorectal cancer. Nevertheless, the effect and regulatory mechanism of HCP5 in laryngeal squamous cell carcinoma (LSCC) remains unknown. In this study, HCP5 expression levels were confirmed to be prominently raised in LSCC cell lines. HCP5 knockdown reduced cell proliferation and migration and invasive ability of LSCC cell lines. Furthermore, miR-216a-5p was confirmed to sponge HCP5, and its expression was prominently downregulated in LSCC cell lines and upregulated in HCP5-silenced LSCC cell lines. miR-216a-5p overexpression downregulated the cell proliferation and migration and invasive ability of LSCC cells. Additionally, the protein level of zinc finger E-box binding homeobox 1 (ZEB1), one target gene of miR-216a-5p, was highly expressed in LSCC cell lines, and its expression level was downregulated by HCP5 knockdown and miR-216a-5p overexpression. An miR-216a-5p inhibitor reversed the effect of HCP5 knockdown on the proliferation and migration and invasive ability of LSCC cells. In conclusion, knocking down HCP5 may be a strategy to suppress the malignant biological function via regulating miR-216a-5p/ZEB1. Therefore, HCP5 may become a prospective therapeutic target for LSCC.

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Section: Discussionmentioning

confidence: 59%

Downregulation of long noncoding RNA HCP5/miR-216a-5p/ZEB1 axis inhibits the malignant biological function of laryngeal squamous cell carcinoma cells

Zhang

Hui²,

Zhao³

et al. 2022

Front. Immunol.

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“…We analyzed each gene in the risk signature, NR2F2-AS1 induced epithelial-mesenchymal transition in non-small cell lung cancer, promoted prostate cancer cell proliferation by regulating CDK4, and modulated the MAPK pathway to regulate colorectal cancer invasion and metastasis [30][31][32]. LINC-PINT inhibited the proliferation and migration of laryngeal squamous cell carcinoma by silencing ZEB1 [33],…”

Section: Discussionmentioning

confidence: 99%

A necroptosis-related lncRNA signature can accurately predict the survival outcome of bladder cancer patients

Zhang

Wang

et al. 2022

Preprint

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Bladder cancer is the second most common malignant tumor in the male genitourinary system. This study explored the prognostic role of necroptosis-related long noncoding RNAs (LncRNAs) in bladder cancer.We used univariate Cox, least absolute shrinkage and selection operator and multivariate Cox regression models to establish a necroptosis-related lncRNA prognostic signature. Then, 13 necroptosis-related lncRNAs were included in risk signature. Patients were divided into the high- and low-risk group based on the median risk score. The risk signature predicted that the areas under the receiver operating characteristic curve of patients at 1 year, 3 years and 5 years were 0.74, 0.78 and 0.79, respectively. Next, nomograms and correction curves were established using risk signature and clinicopathological factors. The nomogram-corrected curve shows a good fit. Gene Set Enrichment Analysis was used to explore the possible molecular mechanisms underlying the different prognosis of the low-risk and high-risk of patients, and showed that tumor-related signaling pathways and intercellular connectivity-related signaling pathways were significantly enriched in the high-risk group, while metabolism-related pathways were enriched in the low-risk group. In addition, Immune cell infiltration analysis and was performed on the two groups of patients and the response to immunotherapy was judged. Finally, tumor mutation data were analyzed, and potentially sensitive chemotherapy drugs were screened. The low-risk group was more sensitive to methotrexate while the patients in the high-risk group were more sensitive to cisplatin, docetaxel, paclitaxel and thapsigargin.

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“…Here, LINC‐PINT can directly interact with EZH2 to enhance H3K27me3‐mediated repression of key genes involved in melanoma progression including CDK1, CCNA2, AURKA, and PCNA (Xu et al, 2019), thus inhibiting the proliferation of melanoma cells. In similar fashion, LINC‐PINT also enriches EZH2 to silence ZEB1 which inhibits the proliferation, migration, and invasion capabilities of laryngeal squamous cell carcinoma cells (Yang et al, 2021). Thus, a common theme is emerging that LINC‐PINT function by recruiting EZH2 to target gene promoters, leading to H3K27 trimethylation and silencing of the genes important for promoting tumorigenicity (Figure 2a).…”

Section: Functional Pintologymentioning

confidence: 98%

“…Restoring LINC‐PINT expression in LSCC cell lines inhibits their proliferation, migration and invasion through the silencing of ZEB1 which involves the activation of EZH2 (Table 1). The levels of phosphorylated AKT/mTOR also declined upon LINC‐PINT overexpression (Yang et al, 2021). The identification of EZH2 involvement is a familiar theme for LINC‐PINT but the independent biological function exerted through AKT/mTOR underscores the multifaceted nature of LINC‐PINT's actions in cancer.…”

Section: Pintology Of Cancermentioning

confidence: 99%

PINTology: A short history of the lncRNA LINC‐PINT in different diseases

et al. 2022

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LINC-PINT is a p53-induced long intergenic noncoding transcript that plays a crucial role in many diseases, especially cancer. This long noncoding RNA (lncRNA) gene produces in total 102 (LNCipedia) alternatively spliced variants (LINC-PINT:1 to LINC-PINT:102). The functions of known variants include RNA transcripts, host transcripts for circular RNA (circRNA) generation and as sources for the translation of short peptides. In most human tumors, LINC-PINT is down-regulated where it serves as a tumor suppressor. However, the diversity of its functions in other maladies signifies its general clinical importance. Current LINC-PINT molecular functions include RNA-protein interactions, miRNA sponging and epigenetic modulation with these mechanisms operating in different cellular contexts to exert effects on biological processes ranging from DNA damage responses, cell cycle and growth arrest, senescence, cell migration and invasion, and apoptosis. Genetic polymorphisms in LINC-PINT have also been functionally associated with cancer and other pathologies including the autoimmune diseases pemphigus foliaceus and arthritis. Hence, LINC-PINT shows great potential as a clinical biomarker, especially for the diagnosis and prognosis of cancer. In this review, we explore the current knowledge highlighting the distinctive molecular functions of LINC-PINT in specific cancers and other disease states.Ihtisham Bukhari and Muhammad Riaz Khan contributed equally to this study.

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LncRNA LINC-PINT Inhibits Malignant Behaviors of Laryngeal Squamous Cell Carcinoma Cells via Inhibiting ZEB1

Cited by 11 publications

References 40 publications

Downregulation of long noncoding RNA HCP5/miR-216a-5p/ZEB1 axis inhibits the malignant biological function of laryngeal squamous cell carcinoma cells

Downregulation of long noncoding RNA HCP5/miR-216a-5p/ZEB1 axis inhibits the malignant biological function of laryngeal squamous cell carcinoma cells

A necroptosis-related lncRNA signature can accurately predict the survival outcome of bladder cancer patients

PINTology: A short history of the lncRNA LINC‐PINT in different diseases

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