LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1

Abstract

Background Long intergenic non-protein coding RNA 342 (LINC00342) has been identified as a novel oncogene, however, the functional role of LINC00342 in colorectal cancer (CRC) remained unclear. Methods The expression of LINC00342 was detected by real-time PCR. Cell proliferation, migration and invasion and xenograft model were examined to analyze the biological functions of LINC00342 in vitro and in vivo. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to identify the target interactions between LINC00342, miR-19a-3p and aminopeptidase like 1 (NPEPL1). Results LINC00342 was highly expressed in CRC. Downregulation of LINC00342 inhibited cell proliferation and metastasis of CRC cells. Moreover, knocking down LINC00342 could weaken the tumor growth in vivo. Mechanistic investigation revealed that LINC00342 may sponge miR-19a-3p to regulate NPEPL1 expression. Further investigation indicated that the oncogenesis facilitated by LINC00342 was inhibited by NPEPL1 depletion.Conclusion LINC00342 promoted CRC progression by competitively binding miR-19a-3p with NPEPL1.

LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1

“…was involved in the ceRNA regulatory network and functioned as an endogenous miRNA sponge to bind to miR-19a-3p and regulated its function. In line with our nding, Chen et al reports that ectopic LINC00342 expression facilitates NSCLC cell proliferation, migration, and invasion by sponging miR-203a-3p [27].…”

“…High expression of LINC00342 predicts a poor prognosis in NSCLC [26]. Knockdown of LINC00342 inhibits NSCLC cell proliferation, migration and invasion [27]. However, the expression and function of LINC00342 were unknown in CRC.…”

MiR-203a-3p regulates TGF-β1-induced epithelial–mesenchymal transition (EMT) in asthma by regulating Smad3 pathway through SIX1

“…For instance, miR-203a-3p inhibited cell proliferation, migration and invasion in lung adenocarcinoma by FBXL19-AS1/miR-203a-3p axis [31]. MiR-203a-3p served as a target of LINC00342 to inhibit cell proliferation, colony formation, migration and invasion in non-small cell lung cancer [32]. Recently, bioinformatics analysis indicated that the expression of miR-203a-3p was dramatically decreased in the bronchial epithelial cells [14].…”

“…Although asthma is commonly diagnosed in childhood, it occurs in all age groups [3]. Ra et al reported that there was no significant difference between the prevalence rate of the elderly (>65) and that of the young people (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34) [4]. A lot of stimuli, including cold air and exercise, and other risk factors such as smoking, obesity and rhinitis contribute to asthma incidence [5][6][7].…”