LncRNA LINC00857 regulates lung adenocarcinoma progression, apoptosis and glycolysis by targeting miR-1179/SPAG5 axis

Wang, Lan; Cao, Liqiang; Wen, Chunxia; Li, Jie; Yu, Guiping; Liu, Chengying

doi:10.1007/s13577-019-00296-8

Cited by 56 publications

(49 citation statements)

References 30 publications

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“…In this research, we discovered that LINC00857 was highly expressed in PAAD samples by analyzing the TCGA database. Accordingly, several researches have revealed that LINC00857 played an essential role in numerous tumors, such as lung adenocarcinoma [10], esophageal adenocarcinoma [11], and hepatocellular carcinoma [12]. However, the understanding on the effect of LINC00857 aberrant expression on PAAD is incomprehensive.…”

Section: Introductionmentioning

confidence: 99%

LncRNA LINC00857 strengthens the malignancy behaviors of pancreatic adenocarcinoma cells by serving as a competing endogenous RNA for miR-340-5p to upregulate TGFA expression

Zhao

Zhou

et al. 2021

PLoS ONE

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Background Pancreatic adenocarcinoma (PAAD) is a pancreatic disease with a high mortality rate in the world. This present research intends to identify the function of lncRNA LINC00857/miR-340-5p/Transforming growth factor alpha (TGFA) in the progression of PAAD. Methods Bioinformatics analysis was used to explore the differentially expressed lncRNA/miRNA/mRNA and analyze the relationship between lncRNA/miRNA/mRNA expression and prognosis of PAAD by enquiring TCGA, GEO and GTEX. KEGG pathway analysis and GO enrichment analysis were implemented to annotate the crucial genes regulated by LINC00857. The biological behaviors of PAAD cells were detected by CCK-8, colony formation and transwell assays. Interactive associations between LINC00857 and miR-340-5p, as well as miR-340-5p and TGFA were analyzed by dual luciferase assay. Results By enquiring TCGA database, we got that LINC00857 was highly expressed in patients with PAAD and positively associated with worse prognosis in PAAD patients. Moreover, LINC00857 upregulation promoted the proliferation and clone formation abilities of PAAD cells. Afterwards, the downstream miRNA and mRNA targets of LINC00857 were picked up to construct a ceRNA network. Further study revealed that TGFA expression was positively regulated by LINC00857 and negatively regulated by miR-340-5p. Besides that, the inhibitory effect of miR-340-5p on PAAD cells growth and movement can be blocked by LINC00857 upregulation. While, the malignant behavior of PAAD cells induced by TGFA overexpression can be eliminated by LINC00857 knockdown. Conclusions Upregulation of LINC00857 improved growth, invasion and migration abilities of PAAD cells by modulation of miR-340-5p/TGFA, affording potential targets and biomarkers for the clinical diagnosis and treatment.

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Section: Introductionmentioning

confidence: 99%

LncRNA LINC00857 strengthens the malignancy behaviors of pancreatic adenocarcinoma cells by serving as a competing endogenous RNA for miR-340-5p to upregulate TGFA expression

Zhao

Zhou

et al. 2021

PLoS ONE

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“…On the contrary, overexpression of SPAG5 gene significantly promoted the viability, proliferation, migration, invasion and inhibition of apoptosis of ESCC cells (Figure 4 and Figure 5). It is reported that cell proliferation, apoptosis, migration and invasion are closely related to the changes of the related protein expression [11,15,16,19,24]. The expression of Caspase-3, Ki67 and VEGF in tumor tissues is closely related to the proliferation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…The proliferation, apoptosis, migration and invasion of tumor cells are the necessary stages of tumor occurrence and development [2,11,15,16,19]. Drug resistance is one of the important reasons for the low survival rate of cancer patients [21].…”

Section: Discussionmentioning

confidence: 99%

“…Many literatures have shown that SPAG5 is abnormally expressed in human tumors and plays an important role in tumorigenesis and development [14,15,16,17]. For example, SPAG5 is significantly upregulated in pancreatic cancer [18], renal, liver cancer [14], cervical cancer [16], non-small cell lung cancer [15] and prostate cancer [19]. Overexpression of SPAG5 promotes the proliferation of tumor cells and inhibits their apoptosis, which is closely related to the poor prognosis of tumor patients.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of sperm associated antigen 5 promotes cell growth, metastasis, and adriamycin resistance in esophageal squamous cell carcinoma via activating PI3K/AKT signaling pathway

Yan¹,

Zhang²,

Yi³

et al. 2020

Preprint

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Background: Esophageal cancer (ESCC) is one of the most common malignant tumors in the digestive system. This study aims to explore the effects of sperm associated antigen 5 (SPAG5) on cell growth, metastasis, and azithromycin resistance in esophagus cancer and its molecular mechanism.Methods: The DEGs were obtained from GSE92396, GSE17351, and GSE9982 datasets about ESCC. The PPI network was constructed using the STRING database and was visualized using Cytoscape software. The cytohubba plug-in of Cytoscape software was used to identify the hub genes of the PPI network. The DEGs were used to perform GO and KEGG pathway enrichment analysis using the DAVID database. Statistical analysis was performed to test the clinical and prognostic significance of SPAG5. Cell viability, proliferation, apoptosis, migration, and invasion were detected using CCK-8, colony formation, flow cytometry, transwell and scratch-wound assays. The expression of related genes was detected by qRT-PCR, western blot and IHC assays. The oncogenicity of SPAG5 in ESCC cells was determined using the nude mouse transplantation tumor experiment.Results: Ninety-three overlapping genes from the DEGs were used to construct the PPI network, and mainly enriched in BP, CC, and MF terms. COX regression analysis of OS showed that SPAG5 expression and pN category were correlated with OS. Univariate and multivariate analyses showed that SPAG5 was an independent prognostic factor for OS in ESCC. The ROC curve analysis showed the AUC of SPAG5 was 0.74. Multiple logistic regression showed that SPAG5 were subsequently identified as an independent risk factor associated with OS. SPAG5 overexpression was detected in ESCC tissues and cell lines, and improved cell proliferation. SPAG5 knockdown reduced cell growth and metastasis and promoted its apoptosis. The functions of SPAG5 overexpression promoting ESCC cell growth and affecting cleaved caspase-3, Ki67, VEGF, and MMP-2/-9 expression were reversed by PI3K/AKT inhibitor. SPAG5 overexpression enhanced resistance to ADM in EC9706 and Eca109 cells and it was closely related to the activation of PI3K/AKT signaling pathway.Conclusion: The overexpression of SPAG5 was an independent good prognostic factor and promoted the proliferation, invasion, migration, and ADM resistance, and inhibited the apoptosis via activating PI3K/AKT signaling pathway in ESCC.

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“…Song et al (27) reported that microRNA (miR)-1179 inhibited SPAG5 expression in NSCLC cells. Wang et al (28) revealed that long non-coding RNA LINC00875 may sponge miR-1179 to regulate SPAG5 expression in LUAD cells. As the in vitro experiments in the present study were only performed in A549 cells with siRNA to knock down SPAG5, further experiments including knockdown and overexpression of SPAG5 should be performed in other LUAD cell lines in order to confirm the conclusions.…”

Section: Discussionmentioning

confidence: 99%

SPAG5 is associated with unfavorable prognosis in patients with lung adenocarcinoma and promotes proliferation, motility and autophagy in A549 cells

Huang

2020

Exp Ther Med

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Sperm-associated antigen 5 (SPAG5) is involved in the tumorigenesis of multiple cancer types. However, the role of SPAG5 during lung adenocarcinoma (LUAD) progression remains to be fully elucidated. In the present study, the expression of SPAG5 in tumor tissues of patients with LUAD from public cancer databases was analyzed using the online software Gene Expression Profiling Interactive Analysis and University of Alabama Cancer Database. The association of SPAG5 expression levels with the prognosis of patients with LUAD was analyzed using Kaplan-Meier Plotter. In addition, the role of SPAG5 in the LUAD cell line A549 was determined by knocking down its expression with specific small interfering RNA. The results demonstrated that SPAG5 expression was upregulated in LUAD tissues and its high expression was associated with unfavorable prognosis. Furthermore, in A549 cells, SPAG5 promoted proliferation, migration, invasion and autophagy, but inhibited apoptosis. The present results suggest that SPAG5 has an oncogenic role in LUAD and may be a potential prognostic predictor and therapeutic target for LUAD.

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LncRNA LINC00857 regulates lung adenocarcinoma progression, apoptosis and glycolysis by targeting miR-1179/SPAG5 axis

Cited by 56 publications

References 30 publications

LncRNA LINC00857 strengthens the malignancy behaviors of pancreatic adenocarcinoma cells by serving as a competing endogenous RNA for miR-340-5p to upregulate TGFA expression

LncRNA LINC00857 strengthens the malignancy behaviors of pancreatic adenocarcinoma cells by serving as a competing endogenous RNA for miR-340-5p to upregulate TGFA expression

Overexpression of sperm associated antigen 5 promotes cell growth, metastasis, and adriamycin resistance in esophageal squamous cell carcinoma via activating PI3K/AKT signaling pathway

SPAG5 is associated with unfavorable prognosis in patients with lung adenocarcinoma and promotes proliferation, motility and autophagy in A549 cells

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