LncRNA MAFG-AS1 Promotes Lung Adenocarcinoma Cell Migration and Invasion by Targeting miR-3196 and Regulating SOX12 Expression

Wu, Qian; Jiang, Jianyang

doi:10.1007/s12033-022-00455-7

Cited by 10 publications

(7 citation statements)

References 22 publications

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“…e involved miRNAs and their target genes need to be uncovered. miR-3196 has been confirmed to regulate lung adenocarcinoma cell migration and invasion via Sox12 [32]. miR-3196 could regulate cell proliferation, migration, and EMT (epithelial-mesenchymal transition) via STRN4 in drug-resistant cells of liver cancer [33].…”

Section: Introductionmentioning

confidence: 99%

Valproic Acid Inhibits Peripheral T Cell Lymphoma Cells Behaviors via Restraining PI3K/AKT Pathway

Peng

Xiong

Dong

2022

Evidence-Based Complementary and Alternative Medicine

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Objective. Alproic acid (VPA) is a clinic antiepileptic drug. Antitumor role of VPA has been studied. The aim of this study was to clarify the treatment effect and potential mechanism of VPA on peripheral T cell lymphomas (PTCLs). Materials and Methods. Hut 78 cells were obtained from the Shanghai Cell Bank, Chinese Academy of Sciences, and randomly divided into six groups: control, VPA (8 mM), empty vector (NC), miR-3196 mimics, miR-3196 inhibitor, and VPA + miR-3196 mimics groups. CCK-8 assay was performed to clarify the regulative role of VPA on cell proliferation. Flow cytometry was applied to determine the apoptotic rate and ROS levels. miR-3196 was tested by RT-qPCR. Western blot was used to test the level of p-PI3K and p-AKT. Biochemical experiments were used to detect changes in the content of ATP, lactate level, and glucose content. Electron microscopy was used to show the structure of mitochondria in Hut 78 cells. Results. VPA greatly promoted the expression of miR-3196 and inhibited cell proliferation in a dose-dependent manner. Compared with the NC group, the cell apoptosis rate, Bax and cleaved-caspase-3 expression, lactate level, ROS expression, and glucose content in the VPA group were significantly increased ( P < 0.05 ), and cell proliferation, ATP production, and the expression of Bcl-2, p-PI3K and p-AKT was decreased significantly ( P < 0.05 ). The role of mir-3196 mimics is similar to VPA. While, the miR-3196 inhibitor had the opposite effect to VPA and mimics. The combination of VPA and miR-3196 mimics has the most obvious effect. Conclusion. VPA can inhibit the proliferation of Hut 78 cells and promote cell apoptosis and the structure and dysfunction of mitochondria by regulating the activity of the PI3K/AKT pathway.

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Section: Introductionmentioning

confidence: 99%

Valproic Acid Inhibits Peripheral T Cell Lymphoma Cells Behaviors via Restraining PI3K/AKT Pathway

Peng

Xiong

Dong

2022

Evidence-Based Complementary and Alternative Medicine

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“…For other types of tumors, Wu et al. recently demonstrated that augmented expression of MAFG-AS1 could promote lung adenocarcinoma cells EMT, proliferation, invasion, and migration by modulating the miR-3196/SOX12 pathway ( 32 ). Additionally, Zhao et al.…”

Section: Discussionmentioning

confidence: 99%

Correlation between long non-coding RNA MAFG-AS1 and cancer prognosis: a meta-analysis

Lin,

Liu,

Lin

et al. 2023

Front. Oncol.

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BackgroundMAF transcription factor G antisense RNA 1 (MAFG-AS1), a novel long non-coding RNA discovered recently, was proved to be useful in predicting malignancy prognosis. Nevertheless, its association with cancer prognosis has been inconsistent. Therefore, this meta-analysis aimed to explore the clinicopathological and prognostic significance of MAFG-AS1 in diverse carcinomas.MethodsStudies focused on MAFG-AS1 expression as a prognostic role in cancers were thoroughly searched in six electronic databases. The value of MAFG-AS1 in malignancies was assessed by hazard ratios (HRs) or odds ratios (ORs). Additionally, the GEPIA database was utilized to further strengthen our conclusion.ResultsA total of 15 studies involving 1187 cases and nine types of cancers were recruited into this meta-analysis. High MAFG-AS1 expression was significantly related to advanced tumor stage (OR = 0.52, 95%CI [0.39, 0.69], P < 0.00001), earlier lymph node metastasis (OR = 3.62, 95%CI [2.19, 5.99], P < 0.00001), worse tumor differentiation (OR = 0.64, 95%CI [0.43, 0.95], P = 0.03), and poor overall survival (HR = 1.94, 95%CI [1.72, 2.19], P < 0.00001). No significant heterogeneity and publication bias was detected across studies. Meanwhile, MAFG-AS1 was significantly elevated in ten kinds of cancers based on the validation of the GEPIA database.ConclusionThe results of this meta-analysis indicated that high MAFG-AS1 expression is dramatically correlated with unfavorable prognosis in cancers. MAFG-AS1 may be served as a promising biomarker for malignancies.

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“…[11][12][13][14] Furthermore, lncRNAs play a role in the growth and infiltration of LUAD cells, resistance to chemotherapy, metastasis, and proliferation. 15 This indicate that lncRNAs could potentially function as indicators for predicting, diagnosing, and prognosing lung cancer. 16 While the prognostic significance of lncRNAs has been documented in lung cancer prognosis, 17,18 there has been relatively limited research on the prognostic implications of lncRNAs linked to m6A methylation in LUAD.…”

Section: Introductionmentioning

confidence: 98%

“…In recent times, in‐depth research has discovered that lncRNAs have a significant impact on the formation and progression of tumors by engaging with various molecules 11–14 . Furthermore, lncRNAs play a role in the growth and infiltration of LUAD cells, resistance to chemotherapy, metastasis, and proliferation 15 . This indicate that lncRNAs could potentially function as indicators for predicting, diagnosing, and prognosing lung cancer 16 .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of m6A modification patterns and m6A‐related lncRNAs as potential biomarkers in lung adenocarcinoma

Wang,

Gu,

et al. 2023

Environmental Toxicology

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BackgroundN6‐methyladenosine (m6A) methylation is considered to induce tumor cell proliferation, migration, and apoptosis. Understanding the mechanism of m6A‐related lncRNAs in the development of lung adenocarcinoma (LUAD) may help predict prognosis.Methodsm6A‐related lncRNAs related to lung cancer were identified and combined with the MeRIP‐Seq dataset. The consensus clustering method was utilized to divide LUAD patients, and prognostic model was constructed using the Lasso Cox algorithm. The cluster profiler package was used for gene ontology and KEGG enrichment. The proportion of immune infiltration was estimated using the CIBERSORT algorithm. The decision tree was constructed by the rpart package, and nomograms were built by the rms package. The Connectivity Map database was analyzed for the therapeutic effects of small molecule drugs for LUAD. In addition, qPCR, colony formation and transwell assays were performed to validate functions of m6A‐associated lncRNAs.ResultsNineteen m6A‐modified lncRNAs in LUAD were identified. LUAD patients were divided into two categories based on the expression of 19 m6A‐related lncRNAs. Cluster 2 patients had better antigen production and expression, while naive B cells, plasma cells, and activated NK cells were lower in cluster 1. Nine m6A‐related lncRNAs were selected to establish a risk model for evaluating the prognosis of LUAD patients. The high‐risk group had higher tumor mutational burden and lower TIDE scores with more gamma delta T cells and neutrophils. Nomograms showed that the prognostic model had predominant predictive ability for LUAD patients based on the risk score analyzed by the decision tree model. Benzo(a)pyrene and neurodazine might improve the prognosis of LUAD patients. The qRT‐PCR results confirmed the reliability of the analytical results.ConclusionThe establishment of a prognostic model of m6A‐related lncRNAs can independently predict overall survival in LUAD and may help to develop personalized immunotherapy strategies.

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LncRNA MAFG-AS1 Promotes Lung Adenocarcinoma Cell Migration and Invasion by Targeting miR-3196 and Regulating SOX12 Expression

Cited by 10 publications

References 22 publications

Valproic Acid Inhibits Peripheral T Cell Lymphoma Cells Behaviors via Restraining PI3K/AKT Pathway

Valproic Acid Inhibits Peripheral T Cell Lymphoma Cells Behaviors via Restraining PI3K/AKT Pathway

Correlation between long non-coding RNA MAFG-AS1 and cancer prognosis: a meta-analysis

Comprehensive analysis of m6A modification patterns and m6A‐related lncRNAs as potential biomarkers in lung adenocarcinoma

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