LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis

Li, Dengbao; Zhong, Siwen; Zhu, Zhiqiang; Jiang, Xinghua; Zhang, Junhao; Gu, Jiang; Chen, Fangmin

doi:10.1159/000509957

Cited by 20 publications

(12 citation statements)

References 44 publications

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“…Firstly, MAFG-DT as a part of lncRNAs signature of tumor-infiltrating B lymphocytes had some predictive value for the survival outcomes and immunotherapy response of patients with antiprogrammed death-1 (PD-1) therapy and added significant predictive power to current immune checkpoint gene markers [ 32 ]. Next, Li et al reported that silencing of MAFG-AS1 inhibited BC cell proliferation, metastasis, and invasion, while overexpression of MAFG-AS1 in BC cell had opposite biological effects, and it was further confirmed that MAFG-AS1/miR-143-3p/COX-2 axis was involved in the progression of BC [ 33 ]. Besides, Xiao et al showed that MAFG-AS1 can promote BC proliferation, invasion, metastasis, and EMT in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 97%

Identification of a Nomogram from Ferroptosis-Related Long Noncoding RNAs Signature to Analyze Overall Survival in Patients with Bladder Cancer

Cui

Chai

Che

et al. 2021

Journal of Oncology

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Purpose. This study aimed to establish a nomogram to predict the overall survival (OS) of patients with bladder cancer (BC) by ferroptosis-related long noncoding RNAs (FRlncRNAs) signature. Methods. We obtained FRlncRNAs expression profiles and clinical data of patients with BC from the Cancer Genome Atlas database. The patients were divided into the training set, testing set, and overall set. Lasso regression and multivariate Cox regression were used to establish the FRlncRNAs signature, the prognosis of each group was compared by Kaplan–Meier (K-M) analysis, and the receiver operating characteristic (ROC) curve evaluated the accuracy of the model. The Gene Set Enrichment Analysis (GSEA) was used for the visualization of the functional enrichment for FRlncRNAs. The databases of GEPIA and K-M Plotter were used for subsequent functional analysis of major FRlncRNAs. Results. Thirteen prognostic FRlncRNAs (LINC00942, MAFG-DT, AL049840.3, AL136084.3, OCIAD1-AS1, AC062017.1, AC008074.2, AC018653.3, AL031775.1, USP30-AS1, LINC01767, AC132807.2, and AL354919.2) were identified to be significantly different, constituting an FRlncRNAs signature. Patients with BC were divided into low-risk group and high-risk group by this signature in the training, testing, and overall sets. K-M analysis showed that the prognosis of patients in the high-risk group was poor and the difference in the subgroup analyses was statistically significant. ROC analysis revealed that the predictive ability of the model was more accurate than traditional assessment methods. A risk score based on FRlncRNAs signature was an independent prognostic factor for the patients with BC (HR = 1.388, 95%CI = 1.228–1.568, P < 0.001 ). Combining the FRlncRNAs signature and clinicopathological factors, a predictive nomogram was constructed. The nomogram can accurately predict the overall survival of patients and had high clinical practicability. The GSEA analysis showed that the primary pathways were WNT, MAPK, and cell-matrix adhesion signaling pathways. The major FRlncRNAs (MAFG-DT) were associated with poor prognosis in the GEPIA and K-M Plotter database. Conclusion. Thirteen prognostic FRlncRNAs and their nomogram were accurate tools for predicting the OS of BC, which might be molecular biomarkers and therapeutic targets.

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Section: Discussionmentioning

confidence: 97%

Identification of a Nomogram from Ferroptosis-Related Long Noncoding RNAs Signature to Analyze Overall Survival in Patients with Bladder Cancer

Cui

Chai

Che

et al. 2021

Journal of Oncology

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“…HOTAIR promotes the proliferation, migration, and invasion of BCa cells by regulating CCNJ and inhibiting miRNA-205 [ 116 ]. MAFG-AS1 regulates the miR-125b-5p/SphK1 and the miR-143-3p/COX-2 axes to promote the proliferation, migration, and invasion of BCa cells [ 117 , 118 ]. SPRY4-IT1 sponges miR-101-3p to promote the proliferation, migration, and invasion of BCa cells by upregulating EZH2 [ 119 ].…”

Section: Invasion Migration and Metastasismentioning

confidence: 99%

“…Increased ARSR expression is positively correlated with higher histological grade and larger tumor size [ 146 ]. SNHG3 [ 137 ], RMRP [ 132 ], PCAT6 [ 189 ], and LSINCT5 [ 149 ] expression positively correlated with tumor size and TNM stage, while high expression of MAFG-AS1 [ 118 , 153 ], SNHG7 [ 90 , 103 ], and TMPO-AS1 [ 33 , 34 ] was closely related to histological grade, tumor size, TNM stage, and clinical stage of BCa patients. However, many lncRNAs are important for tumor size suppression.…”

Section: Lncrnas Are Associated With Clinicopathological Characteristicsmentioning

confidence: 99%

“…In addition, lower expression of 7 lncRNAs predicted shorter OS [ 24 , 26 , 57 , 126 , 150 , 152 , 186 ]. High expression of 28 lncRNAs predicted shorter OS [ 23 , 27 , 32 , 39 , 40 , 45 , 50 , 53 , 60 , 83 , 94 , 100 , 118 , 121 , 123 , 128 , 130 , 137 , 141 , 146 , 155 , 156 , 162 , 164 , 185 , 192 , 194 , 197 ].…”

Section: Lncrnas That Influence Patient Prognosismentioning

confidence: 99%

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Biological functions and clinical significance of long noncoding RNAs in bladder cancer

et al. 2021

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Bladder cancer (BCa) is one of the 10 most common cancers with high morbidity and mortality worldwide. Long noncoding RNAs (lncRNAs), a large class of noncoding RNA transcripts, consist of more than 200 nucleotides and play a significant role in the regulation of molecular interactions and cellular pathways during the occurrence and development of various cancers. In recent years, with the rapid advancement of high-throughput gene sequencing technology, several differentially expressed lncRNAs have been discovered in BCa, and their functions have been proven to have an impact on BCa development, such as cell growth and proliferation, metastasis, epithelial-mesenchymal transition (EMT), angiogenesis, and drug-resistance. Furthermore, evidence suggests that lncRNAs are significantly associated with BCa patients’ clinicopathological characteristics, especially tumor grade, TNM stage, and clinical progression stage. In addition, lncRNAs have the potential to more accurately predict BCa patient prognosis, suggesting their potential as diagnostic and prognostic biomarkers for BCa patients in the future. In this review, we briefly summarize and discuss recent research progress on BCa-associated lncRNAs, while focusing on their biological functions and mechanisms, clinical significance, and targeted therapy in BCa oncogenesis and malignant progression.

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“…20 miR-143-3p was found to demonstrate ectopic expression and was directly related to the progression and prognosis of a variety of cancers. 21,22 Moreover, miR-143-3p was found to be involved in the osteogenic differentiation of BMSCs. 23 However, the role of miR-143-3p in the process of osteogenic differentiation of ADSCs remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA AC092155 facilitates osteogenic differentiation of adipose‐derived stem cells through the miR‐143‐3p/STMN1 axis

Sun

Zhang

Luo

et al. 2021

The Journal of Gene Medicine

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Background: Numerous studies have demonstrated that long noncoding RNAs (lncRNAs) induce osteogenesis in adipose-derived stem cells (ADSCs). This study aimed to explore the role of lncRNAs AC092155 in promoting osteogenic differentiation of ADSCs.Methods: MicroRNA (miRNA) and lncRNA sequencing were performed in ADSCs that underwent normal or osteogenic induction. Differentially expressed miRNAs and lncRNAs were identified using R software. The relative expression levels of lncRNA AC092155, miR-143-3p, and STMN1 during the process of osteogenic induction were determined by real-time polymerase chain reaction (RT-PCR). ADSCs were then transfected with agomiR-143-3p and pcDNA3.1-sh-lncRNA AC092155. Alkaline phosphatase (ALP) and alizarin red staining (ARS) were used to confirm the regulatory function of the lncRNA AC092155/miR-143-3p/STMN1 axis in osteogenic differentiation of ADSCs.Results: lncRNA AC092155 was significantly upregulated in ADSCs following induction in the osteogenic medium. lncRNA AC092155 and STMN1 mimics increase the markers of osteogenic differentiation in the early and late phases, which was reflected in increased ALP activity as well as the higher deposition of calcium nodules. An miR-143-3p mimic showed the opposite effect. Luciferase reporter gene analysis demonstrated that lncRNA AC092155 directly targets miR-143-3p. Moreover, the lncRNA AC092155/miR-143-3p/STMN1 regulatory axis was found to activate the Wnt/β-catenin signaling pathway.Conclusions: lncRNA AC092155 contributes to the osteogenic differentiation of ADSCs. The lncRNA AC092155/miR-143-3p/STMN1 axis may be a new therapeutic target for bone-related diseases.

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LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis

Cited by 20 publications

References 44 publications

Identification of a Nomogram from Ferroptosis-Related Long Noncoding RNAs Signature to Analyze Overall Survival in Patients with Bladder Cancer

Identification of a Nomogram from Ferroptosis-Related Long Noncoding RNAs Signature to Analyze Overall Survival in Patients with Bladder Cancer

Biological functions and clinical significance of long noncoding RNAs in bladder cancer

Long noncoding RNA AC092155 facilitates osteogenic differentiation of adipose‐derived stem cells through the miR‐143‐3p/STMN1 axis

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