LncRNA MAGI2-AS3 and ZEB2 Plays a Crucial Role in the Lung Squamous Cell Carcinoma:a Bioinformatics Study

Abstract

PurposeLung squamous cell carcinoma (LUSC) is a common type of non-small-cell lung cancer. Because of the limitations of targeted therapy and immunotherapy, LUSC treatments are ineffective. To better understand the underlying mechanisms of LUSC carcinogenesis, the present study aimed to identify novel factors and their signaling networks in LUSC, with a primary focus on the construction of a competing endogenous RNA (ceRNA) regulatory network. MethodsWe conducted a transcriptomic analysis of LUSC samples from The Cancer Genome Altas database. Furthermore, we analyzed the data using bioinformatics methods.Results533 samples were selected for analysis, including 485 “PrimaryTumor” samples and 48 “SolidTissueNormal” samples. A total of 1853 DEgenes were identified. Bioinformatics analyses identified the most physiologically relevant and significantly dysregulated lncRNAs and mRNAs. MAGI2-AS3 and ZEB2 were found to play key roles in LUSC. Furthermore, we mapped these signaling pathway based on its role as a miRNA sponge to predict the binding of miRNA to MAGI2-AS3 and ZEB2.ConclusionWe concluded that MAGI2-AS3/ZEB2 loop exhibited tumor-suppressor activity in LUSC by inhibiting miR-374a-5p and miR-374b-5p and modulating the downstream signal transduction through a ceRNA network.