LncRNA MAGI2‐AS3 inhibits hepatocellular carcinoma cell proliferation and migration by targeting the miR‐374b‐5p/SMG1 signaling pathway

Abstract

Long noncoding RNAs (lncRNAs) have been proven to play critical roles in cancer progression. Recently, lncRNA MAGI2‐AS3 has been revealed to be a tumor suppressor and inhibit cell growth by targeting the Fas/FasL signalling pathway in breast cancer. However, the role and underlying mechanism of MAGI2‐AS3 in hepatocellular carcinoma (HCC) remain largely unknown. In the current study, we found that MAGI2‐AS3 expression is downregulated in HCC tissues and closely associated with some clinical characteristics (tumor size, lymph node metastasis, and TNM stage) and poor overall survival. Overexpression of MAGI2‐AS3 inhibits HCC cell proliferation and migration in vitro, while impedes tumor growth in vivo accordantly. In addition, our data suggest that MAGI2‐AS3 could function as an endogenous sponge of miR‐374b‐5p by directly binding to it and suppressing its expression. Furthermore, miR‐374b‐5p upregulation could restore the inhibitory effect of MAGI2‐AS3 on HCC cells processes. Moreover, suppressor with morphogenetic effect on genitalia family member 1 (SMG1) is positively regulated by MAGI2‐AS3 via absorbing miR‐374b‐5p in HCC cells. More important, SMG1 knockdown reverses the suppressive function of MAGI2‐AS3 in HCC cell processes. Taken together, we reveal a functional MAGI2‐AS3/miR‐374b‐5p/SMG1 axis that suppresses HCC progression, potently suggesting a new road for HCC treatment.

Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer

“…The growing evidence shows that PVT1 promotes proliferation, invasion, metastasis, and drug resistance in many cancer cells, and can act as a prognostic indicator . MAGI2‐AS3 is another confirmed lncRNA that can suppress hepatocellular carcinoma cell proliferation and migration by acting as an endogenous sponge of miR‐374b‐5p . NR2F1‐AS1 can regulate hepatocellular carcinoma oxaliplatin resistance through targeting miR‐363‐ABCC1 pathway .…”

“…58 MAGI2-AS3 is another confirmed lncRNA that can suppress hepatocellular carcinoma cell proliferation and migration by acting as an endogenous sponge of miR-374b-5p. 59 regulate hepatocellular carcinoma oxaliplatin resistance through targeting miR-363-ABCC1 pathway. 60 In our analysis, MAGI2-AS3 and NR2F1-AS1 showed highly positive correlation with COL1A1 via miR-143-3p.…”

Long Non-Coding RNA MAGI2-AS3 is a New Player with a Tumor Suppressive Role in High Grade Serous Ovarian Carcinoma

“…The first report showing experimental evidence of MAGI2-AS3 playing a tumor-suppressive role in breast cancer by regulating the Fas/FasL was published in 2018 [28]. Soon thereafter, other reports [31,33,48] demonstrated the tumor-suppressive function of MAGI2-AS3 in bladder cancer hepatocellular cancer and gliomas. Therefore, one of the first aims of this study was to understand if the onco-suppressive role of MAGI2-AS3 extends to EOC, specifically HGSC originating from FT.…”

“…LncRNAs are commonly reported to have competing endogenous RNA function by sponging miRNAs in cytoplasm [16]. To identify MAGI2-AS3 miRNA targets in EOC, using Starbasev3.0 [29] and literature survey [30][31][32][33], a list of demonstrated and predicted miRNA targets was obtained. This was then matched with a list of all the differentially expressed miRNAs between EOC and FT [34,35].…”

“…Figure 5a shows a comparison of these two lists represented as a Venn diagram, and the common miRNAs obtained from both these lists are represented in Figure 5b. Three miRNAs-miR-15b-5p, miR-374a-5p, and miR-374b-5p [30][31][32][33], highlighted in bold, were previously reported to be sponged by MAGI2-AS3 in other cancers, as shown in Supplementary Materials Table S3.…”