LncRNA MALAT1 Promote Cell Proliferation and Invasion by sponging miR-125b to Modulate HMGA1 Expression in Laryngocarcinoma

Abstract

Background: Laryngocarcinoma is the most frequent head and neck malignant tumor. MALAT1 have a role in promoting cell proliferation and metastasis in several tumors. This research aimed to investigate the great roles of MALAT1in laryngocarcinoma.
 Methods: Overall, 54 cases of laryngocarcinoma tissues pathological specimens and paracancerous tissues were collected by surgical resection from the Department of Otolaryngology-Head and Neck Surgery at the Shandong Provincial Hospital affiliated to Shandong University, China from Jan 2012 to Oct 2015. The microRNA and protein levels of genes were evaluated by RT-qPCR and western blot. The proliferative and invasive ability were calculated usingCCK8 and transwell assays. Kaplan-Meier method was used to assess the survival of laryngocarcinoma patients.
 Results: In laryngocarcinoma tissues and cells, lncRNA MALAT1 expression was significantly increased compared to normal tissues and cells. LncRNA MALAT1 promotes proliferation and migration of laryngocarcinoma cells. LncRNA MALAT1 upregulates HMGA1 expression by acting as a competitive endogenous RNA (ceRNA) for miR-125b. Rescue experiments showed that microRNA-125b inhibitor reversed the change in cell viability and invasion induced by sh-MALAT1. Down regulation of lncRNA MALAT1 inhibits laryngocarcinoma proliferation and invasion by modulating miR-125b /HMGA1.
 Conclusion: LncRNA MALAT1 promotes the development of laryngocarcinoma by regulating the expression level of HMGA1 by acting as a miR-125b ceRNA and may be considered as a new strategy for the development of laryngocarcinoma.

Circular RNA circ-ABCB10 Promotes Proliferation and Inhibits Apoptosis of Laryngeal Carcinoma by Inhibiting KLF6

“…Unlike linear RNA with a 5′ cap and a 3′ poly (A) tail at the end, circRNA is characterized by a covalently closed loop structure, which has neither 5′ to 3′ polarity nor a polyadenylated tail, and is highly resistant to exonucleases, so it has high stability and shows tissue-specific expression. Studies have shown that dysfunctional and aberrant circRNA expression is also associated with malignancy [ 11 , 12 ]. Therefore, circRNA is likely to become a potential biomarker for tumors.…”

Interleukin-17 Regulates Keratinocyte Proliferation in Psoriasis Through the MALAT1/miR-125b/BRD4 Axis

“…MALAT1 is highly expressed in mice with acute viral myocarditis, and knockdown of MALAT1 attenuates Th17-related responses, such as the expression of IL-17-related pro-inflammatory factors [26]. The relationship between IL- regulates cell proliferation through miR-125b [30,31], and the expression of miRNA-125b is down-regulated in psoriasis patients [14,16]. Finally, we verified the expression of the MALAT1/miR-125b/BRD4 signaling axis.…”

In silico analysis to identify novel ceRNA regulatory axes associated with gallbladder cancer

“…Literature search on “lncRNA-miRNA pairs” in cancer revealed one lncRNA (MALAT1) -miR125b interaction in laryngocarcinoma ( Zong et al, 2021 ), three lncRNA (TUG1, NEAT1, MALAT1) -miR-34a interactions reported in endometrial cancer, Nasopharyngeal Cancer, Melanoma ( Liu et al, 2017 ; Ji et al, 2019 ; Li et al, 2019 ) and three lncRNA (PVT1, NEAT1, and MALAT1) -miR-30a-5p interactions in papillary thyroid carcinoma, gastric cancer, Hepatocellular Carcinoma ( Feng et al, 2018 ; Pan et al, 2018 ; Rao et al, 2021 ). Previous studies revealed that the lncRNAs H19 and NEAT1 were found to directly target miR-193a-3p in Hepatocellular Carcinoma and Lung Adenocarcinoma, respectively ( Ma et al, 2018 ; Xiong et al, 2018 ).…”