LncRNA MCM3AP-AS1 inhibits cell proliferation in cervical squamous cell carcinoma by down-regulating miRNA-93

Abstract

Background: MCM3AP antisense RNA 1 (MCM3AP-AS1) is characterized as an oncogenic lncRNA in hepatocellular carcinoma and glioblastoma. We analyzed TCGA dataset and observed the down-regulation of MCM3AP-AS1 in cervical squamous cell carcinoma (CSCC). The present study was therefore performed to investigate the role of MCM3AP-AS1 in CSCC. Methods: A total of 64 female patients with CSCC (38–68 years old; mean age: 53.1 ± 6.5 years old) were enrolled in the present study. RT-qPCR was performed to evaluate gene expression. Methylation specific PCR (MSP) was performed to assess the methylation of miR-93 gene after the overexpression and silencing of MCM3AP-AS1. Cell transfections were performed to investigate the interactions between MCM3AP-AS1 and miR-93. Cell proliferation was assessed by CCK-8 assay. Results: The results showed that MCM3AP-AS1 was down-regulated in CSCC and predicted poor survival. The expression levels of MCM3AP-AS1 were inversely correlated with the expression levels of miR-93. Overexpression of MCM3AP-AS1 led to down-regulation of miR-93, while silencing of MCM3AP-AS1 played an opposite role in CSCC cells. Methylation-specific PCR revealed that MCM3AP-AS1 could positively regulate the methylation of miR-93 gene. Cell proliferation analysis showed that overexpression of MCM3AP-AS1 led to reduced proliferation rate of CSCC cells. Silencing of MCM3AP-AS1 played an opposite role and overexpression of miR-93 reduced the effects of overexpressing MCM3AP-AS1. Conclusions: Therefore, MCM3AP-AS1 may inhibit cell proliferation in CSCC by down-regulating miRNA-93.

The MCM3AP-AS1/miR-126/VEGF axis regulates cancer cell invasion and migration in endometrioid carcinoma

“…Therefore, we speculated that MCM3AP-AS1 may play oncogenic roles in cancer biology mainly by serving as a ceRNA of tumor suppressive miRNAs. In addition, a recent study showed that MCM3AP-AS1 was downregulated in cervical cancer and it could downregulate miR-93 to inhibit cell proliferation in cervical cancer [15]. Therefore, MCM3AP-AS1 may play different roles in different cancers.…”

“…Therefore, lncRNAs are potential targets for cancer treatment [11]. LncRNA MCM3AP Antisense RNA 1 (MCM3AP-AS1) plays an oncogenic role in several types of cancer, such as hepatocellular carcinoma, papillary thyroid cancer, glioblastoma, and cervical cancer [12][13][14][15]. It has been well established that MCM3AP-AS1 participates in cancer biology mainly by sponging tumor suppressive miR-NAs to upregulate oncogene expression [12][13][14][15].…”

lncRNA MCM3AP‐AS1 inhibits the progression of colorectal cancer via the miR‐19a‐3p/FOXF2 axis

“…Reportedly, MCM3AP‐AS1 is overexpressed in prostate cancer, and it modulates the methylation of NPY1R promoter via the recruitment of DNMT1/DNMT3, thereby enhancing the multiplication, migration and invasion of cancer cells 22 . However, MCM3AP‐AS1 expression is down‐regulated in cervical squamous cell carcinoma and its reduction is associated with poor survival; functional experiments show that MCM3AP‐AS1 inhibits the multiplication of cells by inhibiting miR‐93 expression 23 . Previously, there have been no reports about the expression pattern, biological function and underlying mechanism of MCM3AP‐AS1 in CRC.…”

circFLT1 and lncCCPG1 Sponges miR-93 to Regulate the Proliferation and Differentiation of Adipocytes by Promoting lncSLC30A9 Expression

“…Previous studies have shown that miR-93 can regulate many cancers, but, to our knowledge, there are no reports on its effects on bovine fat development. 16 , 17 Cioffi et al. reported that miR-93 controls obesity by inhibiting Sirt7 and Tbx3.…”