LncRNA MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer

Liang, Meihua; Jia, Jinliang; Chen, Lili; Wei, Biyue; Guan, Qiang; Ding, Zhaoming; Yu, Jiang; Pang, Rui; He, Gang

doi:10.1007/s12020-019-01939-4

Cited by 70 publications

(86 citation statements)

References 37 publications

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“…For example, (Zhang et al 2019b). Liang et al proved that MCM3AP-AS1 enhanced cell proliferation and invasion in papillary thyroid cancer by targeting miR-211-5p (Liang et al 2019b). Similar to previous studies, we discovered that MCM3AP-AS1 was increased in PC patients and models.…”

Section: Discussionsupporting

confidence: 88%

“…Previously, MCM3AP-AS1 was up-regulated in hepatocellular carcinoma tissues, as well as promoted growth of hepatocellular carcinoma (Wang et al 2019). MCM3AP-AS1 was also found to be elevated in papillary thyroid cancer, and high MCM3AP-AS1 expression stimulated proliferation, migration as well as invasion of papillary thyroid cancer (Liang et al 2019a). Collectively, we speculated MCM3AP-AS1 regulated the progression of tumors.…”

Section: Introductionmentioning

confidence: 99%

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Long non-coding RNA MCM3AP-AS1 promotes growth and migration through modulating FOXK1 by sponging miR-138-5p in pancreatic cancer

Yang

Sun

Guo

et al. 2019

Mol Med

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Background: Pancreatic cancer (PC) is a type of malignant gastrointestinal tumor. Long non-coding RNA MCM3AP antisense RNA 1 (MCM3AP-AS1) has been reported to stimulate proliferation, migration and invasion in several types of tumors. However, the role of MCM3AP-AS1 in PC remains unclear.Methods: MCM3AP-AS1, microRNA miR-138-5p (miR-138-5p) and FOXK1 levels were detected using quantitative real time PCR. Cell proliferation, migration and invasion were analyzed. Dual luciferase reporter assay was used to confirm the relationship between MCM3AP-AS1 and miR-138-5p, between miR-138-5p and FOXK1. Protein levels were identified using western blot analysis. Results: MCM3AP-AS1 overexpression promoted proliferation, migration and invasion in PC cells. MCM3AP-AS1 silencing showed a suppressive effect on cell growth in PC cells. Moreover, MCM3AP-AS1 knockdown suppressed tumor growth in mice. Dual luciferase reporter assay demonstrated MCM3AP-AS1 could sponge microRNA-138-5p (miR-138-5p), and FOXK1 could bind with miR-138-5p. Positive correlation between MCM3AP-AS1 and FOXK1 was testified, as well as negative correlation between miR-138-5p and FOXK1. MCM3AP-AS1 promoted FOXK1 expression by targeting miR-138-5p, and MCM3AP-AS1 facilitated growth and invasion in PC cells by FOXK1. Conclusion: MCM3AP-AS1 promoted growth and migration through modulating miR-138-5p/FOXK1 axis in PC, providing insights into MCM3AP-AS1/miR-138-5p/FOXK1 axis as novel candidates for PC therapy from bench to clinic.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA MCM3AP-AS1 promotes growth and migration through modulating FOXK1 by sponging miR-138-5p in pancreatic cancer

Yang

Sun

Guo

et al. 2019

Mol Med

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“…It has been well established that lncRNA MCM3AP-AS1 plays important roles in the regulation of gene expression, as well as the tumorigenesis and tumor development in hepatocellular carcinoma, 20 glioblastoma, 15 and thyroid cancer. 21 LncRNA MCM3AP-AS1 can affect apoptosis, signaling pathways, tumor metastasis and invasion, which has Figure 3 MiR-543-3p was a regulatory target for lncRNA MCM3AP-AS1. (A) Bioinformatics analysis showed that lncRNA MCM3AP-AS1 had a binding site with miR-543-3p but not MUT construct.…”

Section: Discussionmentioning

confidence: 99%

<p>LncRNA MCM3AP-AS1 Promotes Cell Proliferation and Invasion Through Regulating miR-543-3p/SLC39A10/PTEN Axis in Prostate Cancer</p>

Jia

Bian

et al. 2020

OTT

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Objective: Long-chain noncoding RNAs (lncRNAs) are key players in a wide range of biological processes, especially the pathogenesis and development of tumors. LncRNA MCM3AP-AS1 has been demonstrated to be involved in the invasion of various tumors including prostate cancer (PCa). However, its functions in PCa have not been fully elucidated. Methods: qRT-PCR was conducted to measure the expression levels of lncRNA MCM3AP-AS1 and miR-543-3p in PCa tissue samples and cell lines. The expression levels of E-cadherin and SLC39A10 proteins were detected by Western blots. CCK-8 test, cell scratch test and trans-well test were used to evaluate the proliferation, invasion and migration abilities of PCa cells, respectively. Annexin V-FITC/PI experiments were carried out to determine the status of apoptosis. Bioinformatics analysis and Luciferase assay were used to explore the relationship between lncRNA MCM3AP-AS1, miR-543-3p and SLC39A10. Results: In PCa tissue samples and cell lines, lncRNA MCM3AP-AS1 was up-regulated while miR-543-3p was down-regulated. Over-expression of MCM3AP-AS1 could promote the proliferation and invasion of PCa cells. Correlation analysis showed that the expression of MCM3AP-AS1 and miR-543-3p was significantly and inversely correlated. We further verified that miR-543-3p inhibitor was able to reverse si-MCM3AP-AS1mediated inhibitory effects on the PCa cell proliferation, migration and invasion through regulating the downstream protein axis SLC39A10/PTEN/Akt. Finally, in vivo experiments indicated that knocking down of MCM3AP-AS1 could largely reduce tumor volumes, and decreased the ratio of Ki67-positive cells and the expression of SLC39A10 in tumor samples. Conclusion: LncRNA MCM3AP-AS1 can promote the proliferation, migration and invasion abilities of PCa cells through regulating the miR-543-3p/SLC39A10/PTEN axis, which suggests that lncRNA MCM3AP-AS1 might be a potential target for prostate cancer therapy.

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“…However, the underlying mechanisms remain unclear. Previous studies revealed that lncRNAs play crucial roles in regulating the tumor processes of PTC, such as proliferation, apoptosis, migration and invasion [11][12][13][14][15]. However, the roles of CASC15 in cancer are controversial and elusive.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of long noncoding RNA CASC15 inhibits progression, migration and invasion of papillary thyroid cancer

Shen

Zhang

et al. 2019

Arch Med Sci

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Introduction: Thyroid cancers are the most common malignancy of the endocrine system. Increasing evidence has suggested potential roles for cancer susceptibility candidate 15 (CASC15) in thyroid cancer. Papillary thyroid cancer (PTC) accounts for 80% of thyroid cancer, posing a great threat to public health. The present study aims to investigate the potential roles of CASC15 in PTC. Material and methods: PTC tissues were obtained from patients diagnosed with PTC at the Second Hospital of Hebei Medical University from October 2016 to January 2018. qRT-PCR was performed to examine the mRNA levels of CASC15, Bcl-2/BAX, E-cadherin, caspase-3, and PI3K/AKT. Western blotting was applied to determine the protein levels of PI3K/AKT, CASC15, E-cadherin, BAX, Bcl-2 and caspase-3. CCK-8 assays were used to determine the viability of BCPAP and K1 cancer cells. Wound healing and transwell assays were conducted to evaluate the migration and invasion abilities of BCPAP and K1 cells. Results: The results showed that CASC15 was upregulated in thyroid cancer tissues. Moreover, CASC15 in BCPAP and K1 was decreased by CASC15 siRNA. The progression, migration and invasion of BCPAP cells were significantly decreased after transfection with CASC15 siRNA, which was consistent with that in K1 cells. Furthermore, CASC15 siRNA treatment decreased the level of Bcl-2, and PI3K/AKT, and increased the expression of E-cadherin, BAX, and caspase-3. Conclusions: Downregulated CASC15 inhibited the proliferation, migration and invasion of thyroid cancer. Therefore, CASC15 may be an oncogene in thyroid cancer and may serve as a target marker for the treatment of thyroid cancer.

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LncRNA MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer

Cited by 70 publications

References 37 publications

Long non-coding RNA MCM3AP-AS1 promotes growth and migration through modulating FOXK1 by sponging miR-138-5p in pancreatic cancer

Long non-coding RNA MCM3AP-AS1 promotes growth and migration through modulating FOXK1 by sponging miR-138-5p in pancreatic cancer

<p>LncRNA MCM3AP-AS1 Promotes Cell Proliferation and Invasion Through Regulating miR-543-3p/SLC39A10/PTEN Axis in Prostate Cancer</p>

Downregulation of long noncoding RNA CASC15 inhibits progression, migration and invasion of papillary thyroid cancer

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