LncRNA MEG3 promotes osteogenesis of hBMSCs by regulating miR-21-5p / SOD3 axis

Abstract

Background: This study aimed to investigate the role of long non-coding (Lnc) RNA MEG3 on the osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs). Materials and Methods: The binding of miR-21-5p to LncRNA MEG3 and SOD3 was determined using luciferase reporter assay; fluorescence quantitative PCR was used to detect the expression of LncRNA MEG3 at different induction times. hBMSCs were transfected with LncRNA MEG3 overexpression vector and induced for osteoblasts for 14 days. Alkaline phosphatase (ALP) staining, and alizarin red staining were used to detect bone differentiation, immunofluorescence assays were used to detect the expression of SOD3 and COL2A1. Results: Luciferase reporter assay revealed that miR-21-5p bond to LncRNA MEG3 and SOD3. Flow cytometry analysis showed that hBMSCs were highly pure. After osteogenic induction for 14 days, compared with the control group, the overexpression of LncRNA MEG3 significantly increased the activity of ALP and enhanced the formation of calcium nodules in hBMSCs. The overexpression also increased the expression of COL2A1 and SOD3 significantly (P<0.05). Conclusions: LncRNA MEG3 can promote the osteogenesis and bone regeneration of hBMSCs and increasing the expression of SOD3 and COL2A1 via targeting the miR-21-5p/SOD3 axis

Osteo-immunomodulatory effects of macrophage-derived extracellular vesicles treated with biphasic calcium phosphate ceramics on bone regeneration

“…The levels of pro-angiogenic miRNAs were dramatically enhanced in BEVs, including miR-16-5p [45], miR-17-5p [46], miR-20a-5p [47], miR-21-5p [48], miR-23a-3p [49], miR-26a-5p [50], miR-29b-3p [51], miR-126a-5p [52], miR-146a-5p [53], miR-150a-5p [54], miR-155a-5p [55], miR-204-5p [56], and miR-221-3p [57]. Similarly, the levels of pro-osteogenic miRNAs were notably elevated in BEVs, including miR-16-5p [58], miR-17-5p [59], miR-20a-5p [31], miR-21-5p [60], miR-26a-5p [61], miR-34a-5p [62], miR-146a-5p [53], and miR-218-5p [63]. However, the levels of anti-osteogenic miRNAs like miR-214-3p [64] were diminished in BEVs.…”

Milk-Derived Small Extracellular Vesicles Promote Osteogenic Differentiation and Inhibit Inflammation via microRNA-21

“…MicroRNA-21 (miR-21) is a specific microRNA that plays a vital role in osteogenesis [ 9 ] and inflammation [ 10 ]. Knockout of the miR-21 gene in mice diminished their bone repair capacity, whereas the addition of miR-21 agonists improved bone defect repair [ 11 ].…”