LncRNA MEG3 regulates ASK1/JNK axis-mediated apoptosis and autophagy via sponging miR-23a in granulosa cells of yak tertiary follicles

Han, Xiaobin; Fan, Jinjiang; Wang, Meng; Wang, Yunlong; Wang, Jinglei; Afedo, Seth Yaw; Zhao, Ling; Wang, Yaying; Zhao, Tian; Zhang, Tongxiang; Zhang, Rui; Cui, Yan; Yu, Sijiu

doi:10.1016/j.cellsig.2023.110680

Cited by 7 publications

(4 citation statements)

References 88 publications

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“…First, the primary yGCs were successfully isolated and cultured. Previous studies have confirmed the identity of yGCs through their morphological structure (paving stones) and immunofluorescence (specific FSHR expression) ( 33 , 34 ). In the previous in vitro studies on yGCs, second-generation cells were used for experimental purposes ( 33–35 ).…”

Section: Discussionmentioning

confidence: 74%

“…Previous studies have confirmed the identity of yGCs through their morphological structure (paving stones) and immunofluorescence (specific FSHR expression) ( 33 , 34 ). In the previous in vitro studies on yGCs, second-generation cells were used for experimental purposes ( 33–35 ). In the previous bovine GC culturing procedure ( 36 ), the rate of cell proliferation declined at passage 14 and cell growth plateaued at passage 7 (particularly for cryopreserved and re-thawed cells).…”

Section: Discussionmentioning

confidence: 74%

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Establishment of an immortalized yak granulosa cell line: in vitro tool for understanding the molecular processes of ovarian follicle development

Wen,

Zhou,

Yue

et al. 2024

Front. Vet. Sci.

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The yak, a unique species of cattle found exclusively on the western plateau of China, is a valuable source of livelihood for local residents. However, their low fecundity restricts the expansion of yak farming, whereas regional factors limit studies on yak breeding. Granulosa cells (GCs), which provide essential steroid hormones and growth factors for oocytes, have been the focus of many studies on the mechanisms of follicular growth and atresia. This study aimed to establish an immortalized cell line model that could serve as a tool for future studies on the mechanisms of ovarian follicle development in yaks. First, we isolated primary yak granulosa cells (yGCs) and evaluated their replicative senescence after continuous in vitro subculturing. Subsequently, an immortalized culture method for primary yGC was explored, and a new cell line model was established to study the mechanism of follicular development in vitro. We used a mammalian gene expression lentivirus vector to transfer the simian virus 40 large T antigen (SV40T) into primary yGC to obtain an immortalized cell line. The immortalized yGCs were morphologically identical to the primary yGCs, and cell proliferation and growth were normal within a limited number of generations. Follicle-stimulating hormone receptor (FSHR), a specific marker for GCs, was positively expressed in immortalized yGCs. Furthermore, the immortalized yGCs retained the ability of GCs to synthesize estradiol and progesterone and expressed genes related to steroid synthesis. The establishment of immortalized yGC opens up a myriad of possibilities for advancing our understanding of yak reproductive biology and improving yak breeding strategies.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 74%

Establishment of an immortalized yak granulosa cell line: in vitro tool for understanding the molecular processes of ovarian follicle development

Wen,

Zhou,

Yue

et al. 2024

Front. Vet. Sci.

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show abstract

“…With the survival and survminer R packages, association between subgroups and survival condition was explored. Employing the maftools package (20), we compared the tumor mutation burden (TMB) values of subgroups. In the end, CIBERSORT arithmetic was applied to evaluate tumor immune microenvironment of subgroups.…”

Section: Molecular Subgroup Constructionmentioning

confidence: 99%

“…The long non-coding RNA (lncRNA) is a kind of nonprotein-coding RNA that contains longer than 200 bases [16]. In recent decades, extensive research has consistently demonstrated the significant involvement of long noncoding RNAs (lncRNAs) in cancer development, as well as their contribute to plentiful biological processes like cell proliferation, differentiation, inflammation, and apoptosis [17][18][19][20]. For instance, lncRNA LINC01123 which could interact with c-Myc has been implicated in LC growth and aerobic glycolysis, while this process can also be affect by MiR-199a-5p [21].…”

Section: Introductionmentioning

confidence: 99%

Molecular subgroup establishment and signature creation of lncRNAs associated with acetylation in lung adenocarcinoma

Chen,

Wang,

Shao

et al. 2024

Aging

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Background: The significance of long non-coding RNAs (lncRNAs) as pivotal mediators of histone acetylation and their influential role in predicting the prognosis of lung adenocarcinoma (LUAD) has been increasingly recognized. However, there remains uncertainty regarding the potential utility of acetylation-related lncRNAs (ARLs) in prognosticating the overall survival (OS) of LUAD specimens. Methods: The RNA-Seq and clinical information were downloaded from The Cancer Genome Atlas (TCGA). Through the differential analysis, weighted correlation network analysis (WGCNA), Pearson correlation test and univariate Cox regression, we found out the prognosis associated ARLs and divided LUAD specimens into two molecular subclasses. The ARLs were employed to construct a unique signature through the implementation of the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm. Subsequently, the predictive performance was evaluated using ROC analysis and Kaplan-Meier survival curve analysis. Finally, ARL expression in LUAD was confirmed by quantitative real-time PCR (qRT-PCR). Results: We triumphantly built a ARLs prognostic model with excellent predictive accuracy for LUAD. Univariate and multivariate Cox analysis illustrated that risk model served as an independent predictor for influencing the overall survival OS of LUAD. Furthermore, a nomogram exhibited strong prognostic validity. Additionally, variations were observed among subgroups in the field of immunity, biological functions, drug sensitivity and gene mutations within the field. Conclusions: Nine ARLs were identified as promising indicators of personalized prognosis and drug selection for people suffering with LUAD.

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Quercetin antagonizes apoptosis, autophagy and immune dysfunction induced by di(2-ethylhexyl) phthalate via ROS/ASK1/JNK pathway

Sun,

Wang,

et al. 2024

Comparative Biochemistry and Physiology Part C: Toxicology &

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LncRNA MEG3 regulates ASK1/JNK axis-mediated apoptosis and autophagy via sponging miR-23a in granulosa cells of yak tertiary follicles

Cited by 7 publications

References 88 publications

Establishment of an immortalized yak granulosa cell line: in vitro tool for understanding the molecular processes of ovarian follicle development

Establishment of an immortalized yak granulosa cell line: in vitro tool for understanding the molecular processes of ovarian follicle development

Molecular subgroup establishment and signature creation of lncRNAs associated with acetylation in lung adenocarcinoma

Quercetin antagonizes apoptosis, autophagy and immune dysfunction induced by di(2-ethylhexyl) phthalate via ROS/ASK1/JNK pathway

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