lncRNA MIAT promotes cell invasion and migration in esophageal cancer

Zhang, Weiguo; Chen, Qiang; Lei, Caipeng

doi:10.3892/etm.2020.8588

Cited by 15 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…New observations have emerged in the control of immune checkpoint molecules: through hsa-miR-150-5p sponge interaction, lncRNA-MIAT, together with HLA complex P5 ( HCP5 ), has been associated with the up-regulated expression of PD-L1/CD274 , suggesting new involvements in the field of tumor immunity and immunotherapy [ 123 ]. Independently from the ceRNA mechanism, the same lnc-RNA MIAT molecules were characterized as oncogenic in esophageal cancer, as they promote cell invasion and migration by interacting with histone methyltransferase mixed-lineage leukemia ( MLL ) proteins [ 200 ] and, moreover, in gastric cancer, they have been linked to the prognosis and survival predictions: for instance, high MIAT level in serum exosomal characterizes patients as more prone to develop gastric cancer and its up-regulation is associated with shorter survival periods and represents an independent prognostic factor for gastric cancer [ 201 ].…”

Section: Discussionmentioning

confidence: 99%

Competing Endogenous RNAs, Non-Coding RNAs and Diseases: An Intertwined Story

Ala

2020

Cells

130

View full text Add to dashboard Cite

MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are responsible for RNA silencing and post-transcriptional regulation of gene expression. They can mediate a fine-tuned crosstalk among coding and non-coding RNA molecules sharing miRNA response elements (MREs). In a suitable environment, both coding and non-coding RNA molecules can be targeted by the same miRNAs and can indirectly regulate each other by competing for them. These RNAs, otherwise known as competing endogenous RNAs (ceRNAs), lead to an additional post-transcriptional regulatory layer, where non-coding RNAs can find new significance. The miRNA-mediated interplay among different types of RNA molecules has been observed in many different contexts. The analyses of ceRNA networks in cancer and other pathologies, as well as in other physiological conditions, provide new opportunities for interpreting omics data for the field of personalized medicine. The development of novel computational tools, providing putative predictions of ceRNA interactions, is a rapidly growing field of interest. In this review, I discuss and present the current knowledge of the ceRNA mechanism and its implications in a broad spectrum of different pathologies, such as cardiovascular or autoimmune diseases, cancers and neurodegenerative disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Competing Endogenous RNAs, Non-Coding RNAs and Diseases: An Intertwined Story

Ala

2020

Cells

130

View full text Add to dashboard Cite

show abstract

“…Human ESCA cell lines Eca109 and TE-1 and normal esophageal epithelial cell line HEsEpiC were bought from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All cells were incubated in Roswell Park Memorial Institute 1640 (RPMI-1640; Thermo Fisher Scientific) supplementing with 10% fetal bovine serum (FBS, Invitrogen), 100 mg/ml streptomycin and 100 U/ml penicillin at 37°C with 5% CO 2 [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Long noncoding RNA KCNMB2-AS1 promotes the development of esophageal cancer by modulating the miR-3194-3p/PYGL axis

Liu

et al. 2021

Bioengineered

View full text Add to dashboard Cite

Esophageal cancer (ESCA), as a common cancer worldwide, is a main cause of cancer-related mortality. Comprehensive studies on molecular mechanism of ESCA have been carried out. Though numerous long noncoding RNAs (lncRNAs) was reported to participate in the occurrence and development of ESCA, the potential role of lncRNA potassium calcium-activated channel subfamily M regulatory beta subunit 2 (KCNMB2) antisense RNA 1 (KCNMB2-AS1) in ESCA remains to be discovered. This study intends to investigate the detailed function and molecular mechanism of KCNMB2-AS1 in ESCA. Gene expression was evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Cell proliferation was examined by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell invasion and migration were measured by wound healing assay and Transwell assay. Luciferase reporter assay was adopted to validate the interaction between KCNMB2-AS1 and miR-3194-3p. Western blotting was performed to assess protein levels. We discovered that KCNMB2-AS1 was significantly upregulated in ESCA. KCNMB2-AS1 downregulation suppressed the growth, invasion, migration and stemness of ESCA cells. KCNMB2-AS1 bound with miR-3194-3p, and glycogen phosphorylase L (PYGL) was a direct target of miR-3194-3p. KCNMB2-AS1 upregulated PYGL expression by directly binding with miR-3194-3p. Additionally, PYGL overexpression abolished the inhibitory influence of KCNMB2-AS1 depletion on ESCA cell behaviors. Collectively, lncRNA KCNMB2-AS1 promotes ESCA development through targeting the miR-3194-3p/ PYGL axis, which might provide theoretical basis to explore novel biomarkers for ESCA treatment.

show abstract

“…Myocardial infarction-associated transcript (MIAT), located on human chromosome 22 (19), was originally discovered as a gene associated with the risk of myocardial infarction. Zhang et al (24) knocked out MIAT and found that the survival rate of esophageal cancer cells was reduced, the expression of cyclin D3 and CDK2 was reduced, and cell cycle arrest occurred in the G1 phase, leading to the inhibition of cell proliferation. Further, the levels of MMP2 and MMP9 were significantly downregulated, and metastasis was inhibited.…”

Section: Long-chain Non-coding Rnas Promote the Occurrence And Develo...mentioning

confidence: 99%

Long Non-Coding RNA in Esophageal Cancer: A Review of Research Progress

Yang¹,

Chen²

2022

Pathol. Oncol. Res.

View full text Add to dashboard Cite

In recent years, there has been significant progress in the diagnosis and treatment of esophageal cancer. However, owing to the lack of early diagnosis strategies and treatment targets, the prognosis of patients with esophageal cancer remains unsatisfactory. There is an urgent need to identify novel biomarkers and treatment targets for esophageal cancer. With the development of genomics, long-chain non-coding RNAs (LncRNAs), which were once considered transcriptional “noise,” are being identified and characterized rapidly in large numbers. Recent research shows that LncRNAs are closely related to a series of steps in tumor development and play an important regulatory role in DNA replication, transcription, and post-transcriptional regulation. The abnormal expression of LncRNAs leads to tumor cell proliferation, migration, invasion, and treatment resistance. This review focuses on the latest progress in research on the abnormal expression and functional mechanisms of LncRNAs in esophageal cancer. Further, it discusses the potential applications of these findings towards achieving an early diagnosis, improving treatment efficacy, and evaluating the prognosis of esophageal cancer.

show abstract

lncRNA MIAT promotes cell invasion and migration in esophageal cancer

Cited by 15 publications

References 35 publications

Competing Endogenous RNAs, Non-Coding RNAs and Diseases: An Intertwined Story

Competing Endogenous RNAs, Non-Coding RNAs and Diseases: An Intertwined Story

Long noncoding RNA KCNMB2-AS1 promotes the development of esophageal cancer by modulating the miR-3194-3p/PYGL axis

Long Non-Coding RNA in Esophageal Cancer: A Review of Research Progress

Contact Info

Product

Resources

About