LncRNA NEAT1 promotes gastric cancer progression via miR-1294/AKT1 axis

Abstract

Long non-coding RNAs (lncRNAs) were reported to promote the development of gastric cancer (GC). Nuclear-enriched abundant transcript 1 (NEAT1) played a great role in diverse cancers, but the mechanism of NEAT1 in GC remains indistinct. NEAT1 and AKT1 were distinctly up-regulated and miR-1294 was down-regulated in GC tissues and cells. Cell proliferation and metastasis were refrained but apoptosis was promoted in GC cells after knockdown of NEAT1. NEAT1 negatively regulated miR-1294 expression, and the miR-1294 inhibitor reverted the si-NEAT1-induced effect on GC cells. NEAT1 modulated AKT1 expression through miR-1294, and the si-NEAT1-induced effect was relieved by AKT1. NEAT1 affected phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway via regulating miR-1294 and AKT1. NEAT1 could modulate cell proliferation, apoptosis, and metastasis in GC cells by regulating the PI3K/AKT/mTOR signaling pathway via the miR-1294/AKT1 axis, showing the great potential for NEAT1 as a valid biomarker in the progression and treatment of GC.

Depressing hsa_circ_0058092 functions an integrated anti-proliferation and anti-motility role in gastric cancer partially through targeting miR-1294/SIX1 axis

“…In this study, expression of miR-1294 was distinctively downregulated in GC tumors and its expression was moderately correlated with hsa_circ_0058092 level. The downregulation of miR-1294 in this cohort of GC patients supported previous findings [21,32,33]. Higher miR-1294 level showed a significant longer diseasefree survival and overall survival, as well as less metastasis [21,32].…”

“…Higher miR-1294 level showed a significant longer diseasefree survival and overall survival, as well as less metastasis [21,32]. Our data displayed an inhibitory effect of miR-1294 overexpression on GC cell proliferation and motility; blocking miR-1294 recovered GC tumor cell behaviors under hsa_circ_0058092 knockdown condition, which was similar to the rescue effect of miR-1294 inhibitor in NEAT1-silenced GC cells [33]. Besides, the finding that miR-1294 upregulation was detrimental to EMT, invasion and wound closure in GC cells was in favor of Wang et al [32].…”

lncRNA ACTA2-AS1 inhibits malignant phenotypes of gastric cancer cells

“…Then, plasmid DNA-lipid complexes were prepared and incubated at room temperature for 5 min. Next the complexes were supplemented to cells [ 32 ]. The efficiency was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) after 48 h [ 27 ].…”

Molecular Interactions of the Long Noncoding RNA NEAT1 in Cancer

“…Gastric cancer is the third leading cause of cancer-related death worldwide [ 48 ]. Although early treatment of gastric cancer has achieved significant success, early diagnosis is still difficult; most patients present with an advanced stage at the time of diagnosis, causing a poor survival rate [ 49 , 50 , 51 ].…”