LncRNA NUTM2A-AS1 aggravates the progression of hepatocellular carcinoma by activating the miR-186-5p/KLF7-mediated Wnt/beta-catenin pathway

Long, Jianwu; Liu, Longfei; Yang, Xuefeng; Zhou, Xiaojun; Lu, Xianzhou; Qin, Lei

doi:10.1007/s13577-022-00802-5

Cited by 9 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Approximately 60% of the NUTM2A-AS1::RP11-203L2.4 containing tumor cells were solely identified by long read evidence, another 20% by short reads only, and the remaining 20% by both short and long reads ( Figure 5b ). Interestingly, fusion gene partner NUTM2A-AS1 has recently been identified as an oncogene with roles in multiple cancer types (Wang et al 2020; Wang et al 2021; Long et al 2023). The long fusion reads appear to be largely full-length and yield evidence for eight different fusion splicing isoforms, mostly involving skipping of alternative exons and one isoform involving an alternative terminal exon ( Figure 5c ).…”

Section: Resultsmentioning

confidence: 99%

CTAT-LR-fusion: accurate fusion transcript identification from long and short read isoform sequencing at bulk or single cell resolution

Qin,

Popic,

et al. 2024

Preprint

View full text Add to dashboard Cite

Gene fusions are found as cancer drivers in diverse adult and pediatric cancers. Accurate detection of fusion transcripts is essential in cancer clinical diagnostics, prognostics, and for guiding therapeutic development. Most currently available methods for fusion transcript detection are compatible with Illumina RNA-seq involving highly accurate short read sequences. Recent advances in long read isoform sequencing enable the detection of fusion transcripts at unprecedented resolution in bulk and single cell samples. Here we developed a new computational tool CTAT-LR-fusion to detect fusion transcripts from long read RNA-seq with or without companion short reads, with applications to bulk or single cell transcriptomes. We demonstrate that CTAT-LR-fusion exceeds fusion detection accuracy of alternative methods as benchmarked with simulated and real long read RNA-seq. Using short and long read RNA-seq, we further apply CTAT-LR-fusion to bulk transcriptomes of nine tumor cell lines, and to tumor single cells derived from a melanoma sample and three metastatic high grade serous ovarian carcinoma samples. In both bulk and in single cell RNA-seq, long isoform reads yielded higher sensitivity for fusion detection than short reads with notable exceptions. By combining short and long reads in CTAT-LR-fusion, we are able to further maximize detection of fusion splicing isoforms and fusion-expressing tumor cells. CTAT-LR-fusion is available at https://github.com/TrinityCTAT/CTAT-LR-fusion/wiki.

show abstract

Section: Resultsmentioning

confidence: 99%

CTAT-LR-fusion: accurate fusion transcript identification from long and short read isoform sequencing at bulk or single cell resolution

Qin,

Popic,

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…KLFs is a class of transcription factors, which plays an important role in the regulation of cell proliferation, differentiation and embryonic development [19]. Currently, some studies have proved that KLF7 is highly correlated with the occurrence and development of tumor cells [20], and it can be used as a molecular marker for some cancers with adverse effects after rain. The level of KLF7 was significantly increased in HCC cells and was negatively correlated with miR-186-5p.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Circ_0001971 Aggravates the Progression of Hepatocellular Carcinoma Cells by Activating the miR-186-5p/KLF7

Zeng¹

2023

IJCEMR

View full text Add to dashboard Cite

Background: Circ_0001971 has been shown to play an important role in cancer development, yet its mechanism of action in hepatocellular carcinoma (HCC) remains somewhat unknown. Methods: RNA sequence analysis was performed on HCC tissues and paired non-tumor tissues to detect differentially expressed Rnas. qPCR was used to detect the expression level of circ_0001971. CCK-8, Transwell, RNA immunoprecipitation experiment, WB detection, Double luciferase reporter gene assay et al. were used to analyze the interaction between circ_0001971 and miR-186-5p/KLF7. Results: Circ_0001971 expression was significantly upregulated in HCC cells; circ_0001971 inhibition significantly inhibited proliferation, migration and invasion of HCC cells (P<0.05); miR-186-5p was a downstream target of circ_0001971; KLF7 was a target gene of miR-186-5p; circ_0001971 can indirectly positively regulate KLF7. Conclusions: Circ_0001971 aggravates HCC progression by activating miR-186-5p/KLF7.

show abstract

“…SNHG12 ( Chen et al, 2021 ), PRR34-AS1 ( Qin et al, 2021 ) and NUTM2A-AS1 ( Long et al, 2023 ) axes induce EMT via Wnt/β-catenin signaling. Furthermore, Huang et al (2020) point out that miR-1258 is downregulated in HCC patients.…”

Section: Emt-related Ncrnas Mechanisms Of Actionmentioning

confidence: 99%

“…DARS-AS1 induces EMT via interacting with miR-3200-5p, further promoting Cytoskeleton associated protein 2 (CKAP2) expression and FAK/ ERK pathway activation (Feng et al, 2021). SNHG12 , PRR34-AS1 (Qin et al, 2021) and NUTM2A-AS1 (Long et al, 2023) axes induce EMT via Wnt/βcatenin signaling. Furthermore, point out that miR-1258 is downregulated in HCC patients.…”

Section: Lncrna/mirna/mrna Axesmentioning

confidence: 99%

The pivotal role of EMT-related noncoding RNAs regulatory axes in hepatocellular carcinoma

Ghionescu,

Sorop,

Dima

2023

Front. Pharmacol.

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) remains a major health problem worldwide, being the leading cause of cancer-related deaths, with limited treatment options, especially in its advanced stages. Tumor resistance is closely associated with the activation of the EMT phenomenon and its reversal, being modulated by different molecules, including noncoding RNAs (ncRNAs). Noncoding RNAs have the potential to function as both tumor suppressors and oncogenic molecules, controlling the malignant potential of HCC cells. Basically, these molecules circulate in the tumor microenvironment, encapsulated in exosomes. Their impact on cell biology is more significant than originally expected, which makes related research rather complex. The temporal and spatial expression patterns, precise roles and mechanisms of specific ncRNAs encapsulated in exosomes remain primarily unknown in different stages of the disease. This review aims to highlight the recent advances in ncRNAs related to EMT and classifies the described mechanism as direct and indirect, for a better summarization. Moreover, we provide an overview of current research on the role of ncRNAs in several drug resistance-related pathways, including the emergence of resistance to sorafenib, doxorubicin, cisplatin and paclitaxel therapy. Nevertheless, we comprehensively discuss the underlying regulatory mechanisms of exosomal ncRNAs in EMT-HCC via intercellular communication pathways.

show abstract

LncRNA NUTM2A-AS1 aggravates the progression of hepatocellular carcinoma by activating the miR-186-5p/KLF7-mediated Wnt/beta-catenin pathway

Cited by 9 publications

References 39 publications

CTAT-LR-fusion: accurate fusion transcript identification from long and short read isoform sequencing at bulk or single cell resolution

CTAT-LR-fusion: accurate fusion transcript identification from long and short read isoform sequencing at bulk or single cell resolution

Circular RNA Circ_0001971 Aggravates the Progression of Hepatocellular Carcinoma Cells by Activating the miR-186-5p/KLF7

The pivotal role of EMT-related noncoding RNAs regulatory axes in hepatocellular carcinoma

Contact Info

Product

Resources

About