LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration

Jiang, Xingzhi; Ye, Zhongxue; Jiang, Yafen; Yu, Weiyang; Qian, Fang

doi:10.1186/s40659-020-00315-1

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…LINC00174 targets miR-4500 in laryngeal papilloma to inhibit BZW2 and promote tumor cell proliferation (68). OIP5-AS1 promotes the invasion and migration of ovarian cancer cells (69). These reports further hint at the feasibility of our analysis.…”

Section: Discussionmentioning

confidence: 65%

Comprehensive Analysis of GLUT1 Immune Infiltrates and ceRNA Network in Human Esophageal Carcinoma

Liu

Gao

et al. 2021

Front. Oncol.

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BackgroundGlucose transporter 1 (GLUT1) is encoded by the solute carrier family 2A1 (SLC2A1) gene and is one of the glucose transporters with the greatest affinity for glucose. Abnormal expression of GLUT1 is associated with a variety of cancers. However, the biological role of GLUT1 in esophageal carcinoma (ESCA) remains to be determined.MethodsWe analyzed the expression of GLUT1 in pan-cancer and ESCA as well as clinicopathological analysis through multiple databases. Use R and STRING to perform GO/KEGG function enrichment and PPI analysis for GLUT1 co-expression. TIMER and CIBERSORT were used to analyze the relationship between GLUT1 expression and immune infiltration in ESCA. The TCGA ESCA cohort was used to analyze the relationship between GLUT1 expression and m6A modification in ESCA, and to construct a regulatory network in line with the ceRNA hypothesis.ResultsGLUT1 is highly expressed in a variety of tumors including ESCA, and is closely related to histological types and histological grade. GO/KEGG functional enrichment analysis revealed that GLUT1 is closely related to structural constituent of cytoskeleton, intermediate filament binding, cell-cell adheres junction, epidermis development, and P53 signaling pathway. PPI shows that GLUT1 is closely related to TP53, GIPC1 and INS, and these three proteins all play an important role in tumor proliferation. CIBERSORT analysis showed that GLUT1 expression is related to the infiltration of multiple immune cells. When GLUT1 is highly expressed, the number of memory B cells decreases. ESCA cohort analysis found that GLUT1 expression was related to 7 m6A modifier genes. Six possible crRNA networks in ESCA were constructed by correlation analysis, and all these ceRNA networks contained GLUT1.ConclusionGLUT1 can be used as a biomarker for the diagnosis and treatment of ESCA, and is related to tumor immune infiltration, m6A modification and ceRNA network.

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Section: Discussionmentioning

confidence: 65%

Comprehensive Analysis of GLUT1 Immune Infiltrates and ceRNA Network in Human Esophageal Carcinoma

Liu

Gao

et al. 2021

Front. Oncol.

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“…As a long-chain non-coding RNA, OIP5-AS1 plays an essential regulatory role in the development of tumors, and its regulatory role in cell proliferation, migration, invasion and other cellular functions has been con rmed. OIP5-AS1 is overexpressed in ovarian cancer, and overexpression of OIP5-AS1 as a molecular sponge adsorbs miR-34a to promote tumor cell migration and invasion (48). The expression of OIP5-AS1 was increased in pancreatic ductal adenocarcinoma.…”

Section: Discussionmentioning

confidence: 98%

SUMOylation of IGF2BP2 Promotes Vasculogenic Mimicry of Glioma Via Regulating OIP5-AS1/miR-495-3p

Wang

et al. 2021

Preprint

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Background: Glioma is the most common primary malignant tumor of human central nervous system, and its rich vascular characteristics make anti-angiogenic therapy become a therapeutic hotspot. However, the existence of glioma VM makes the anti-angiogenic therapy ineffective. SUMOylation is a post-translational modification that affects cell tumorigenicity by regulating the expression and activity of substrate proteins.Methods: The binding and modification of IGF2BP2 and SUMO1were identified using Ni2+-NTA agarose bead pull-down assays, CO-IP and western blot; and in vitro SUMOylation assays combined with immunoprecipitation and Immunofluorescence staining were performed to explore the detail affects and regulations of the SUMOylation on IGF2BP2. RT-PCR and western blot were used to detect the expression levels of IGF2BP2, OIP5-AS1, and miR-495-3p in glioma tissues and cell lines. CCK-8 assays, cell transwell assays, and three-dimensional cell culture methods were used for evaluating the function of IGF2BP2, OIP5-AS1, miR-495-3p, HIF1A and MMP14 in biological behaviors of glioma cells. Meantime, RIP and luciferase reporter assays were used forinquiring into the interactions among IGF2BP2, OIP5-AS1, miR-495-3p, HIF1A and MMP14. Eventually, the tumor xenografts in nude mice further ascertained the effects of IGF2BP2 SUMOylation on glioma cells.Results: This study proved that IGF2BP2 mainly binds to SUMO1 and was SUMOylated at the lysine residues K497, K505 and K509 sites, which can be reduced by SENP1. SUMOylation increased IGF2BP2 protein expression and blocked its degradation through ubiquitin-proteasome pathway, thereby increasing its stability. The expressions of IGF2BP2 and OIP5-AS1 were up-regulated and the expression of miR-495-3p was down-regulated in both glioma tissues and cells. IGF2BP2 enhances the stability of OIP5-AS1, thereby increasing the binding of OIP5-AS1 to miR-495-3p, weakening the binding of miR-495-3p to the 3’UTR of HIF1A and MMP14 mRNA, and ultimately promoting the formation of VM in glioma.Conclusions: This study first revealed that SUMOylation of IGF2BP2 regulated OIP5-AS1/miR-495-3p to promote VM formation in glioma cells and xenografts growth in nude mice, providing a new idea for molecular targeted therapy of glioma.

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“… 47 Massive amounts of evidence proved that lncRNAs enhance the migratory and invasive abilities of OC or SOC cells via regulating the EMT‐regulated genes. For instance, lncRNA OIP5‐AS1 up‐regulates Snail expression to promote OC cell invasion and migration, 48 lncRNA EBIC promotes metastasis of OC cells through up‐regulating the E‐cadherin expression, 49 and lncRNA HAL suppresses the metastasis of SOC cells by regulating the expressions of E‐cadherin and N‐cadherin to inhibit EMT signaling pathway. 50 Consistent with the results described in the previous literature, our study discovered that LIFR‐AS1 overexpression facilitated an anti‐metastatic phenotype in SOC by regulating EMT‐related genes.…”

Section: Discussionmentioning

confidence: 99%

LncRNA LIFR‐AS1 overexpression suppressed the progression of serous ovarian carcinoma

Liu

Cao

Zhang

et al. 2022

Clinical Laboratory Analysis

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Background Serous ovarian carcinoma (SOC) is a common malignant tumor in female reproductive system. Long noncoding RNA (lncRNA) LIFR‐AS1 is a tumor suppressor gene in colorectal cancer, but its effect and underlying mechanism in SOC are still unclear. Therefore, this study focuses on unveiling the regulatory mechanism of LIFR‐AS1 in SOC. Methods The relationship between LIFR‐AS1 expression and prognosis of SOC patients was analyzed by TCGA database and Starbase, and then, the LIFR‐AS1 expression in SOC tissues and cells was detected by quantitative real‐time PCR (qRT‐PCR) and in situ hybridization (ISH). Besides, the relationship between LIFR‐AS1 and clinical characteristics was analyzed. Also, the effects of LIFR‐AS1 on the biological behaviors of SOC cells were measured by Cell Counting Kit‐8, colony formation, and wound‐healing and Transwell assays, respectively. Western blot and qRT‐PCR were employed to determine the protein expressions of genes related to proliferation (PCNA), apoptosis (cleaved caspase‐3), epithelial‐mesenchymal transition (E‐cadherin, N‐cadherin, and Snail). Results LIFR‐AS1 was lowly expressed in SOC, which was correlated with the poor prognosis of SOC patients. Low expression of LIFR‐AS1 in SOC was associated with the tumor size, clinical stage, lymph node metastasis, and distant metastasis. LIFR‐AS1 overexpression promoted the expressions of cleaved caspase‐3 and E‐cadherin while suppressing the malignant behaviors (proliferation, migration, and invasion) of SOC cells, the expressions of PCNA, N‐cadherin, and Snail. Besides, silencing LIFR‐AS1 exerted the effects opposite to overexpressed LIFR‐AS1. Conclusion LIFR‐AS1 overexpression inhibits biological behaviors of SOC cells, which may be a new therapeutic method.

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LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration

Cited by 15 publications

References 24 publications

Comprehensive Analysis of GLUT1 Immune Infiltrates and ceRNA Network in Human Esophageal Carcinoma

Comprehensive Analysis of GLUT1 Immune Infiltrates and ceRNA Network in Human Esophageal Carcinoma

SUMOylation of IGF2BP2 Promotes Vasculogenic Mimicry of Glioma Via Regulating OIP5-AS1/miR-495-3p

LncRNA LIFR‐AS1 overexpression suppressed the progression of serous ovarian carcinoma

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