LncRNA RUNX1-IT1 is Downregulated in Endometrial Cancer and Binds to miR-21 Precursor to Suppress Its Maturation

Mao, Liang; Wang, Hongbo; Liu, Cong; Tao, Lei; Min, Jie

doi:10.2147/cmar.s272165

Cited by 8 publications

(2 citation statements)

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“…LncRNA RUNX1-IT1 is a newly identified non-coding RNA with an important regulatory function, and can inhibit or promote cancer in different tumors; for example, it is lowly expressed in liver cancer, endometrial cancer, and colorectal cancer, but it is highly expressed in pancreatic cancer and glioma ( 11 , 13 , 14 , 20 , 21 ). The lncRNA RUNX1-IT1 expression level in liver cancer tissues is significantly reduced, and the overexpression of lncRNA RUNX1-IT1 can limit the growth of liver cancer cells and induce their apoptosis.…”

Section: Discussionmentioning

confidence: 99%

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The molecular mechanism of long non-coding ribonucleic acid (lncRNA) RUNX1-IT1 promotes the proliferation and stemness of lung cancer cells

Miao¹,

Li²,

Liu³

et al. 2021

Transl Cancer Res TCR

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Background: This study sought to explore the role of long non-coding ribonucleic acid (lncRNA) RUNX1-IT1 in lung cancer proliferation and cell stemness and clarify its molecular mechanism.Methods: Quantitative reverse transcription polymerase chain reaction was used to detect the expression levels of lncRNA RUNX1-IT1 in lung cancer cell lines and tissues. Cell Counting Kit 8, a plate cloning experiment, a cell suspension sphere-forming assay and a Transwell assay were used to identify the effects of lncRNA RUNX1-IT1 overexpression or down-expression on clone formation, cell progression, cell stemness, and invasion. Western blot was used to detect the expression of associated proteins that regulate cell invasion and stemness.Results: Low expression levels of lncRNA RUNX1-IT1 were detected in the cancerous lung cells and tissues. The overexpression of lncRNA RUNX1-IT1 significantly restricted the ability of cells to proliferate, produce clones, form spheres, and invade lung cancer cells, while the knockdown of lncRNA RUNX1-IT1 had the opposite effect. The findings of the Western blot assessment showed that the overexpression or knockdown of lncRNA RUNX1-IT1 significantly affected the expression of cluster of differentiation 44, cluster of differentiation 133, sex-determining region Y-box 2, octamer-binding transcription factor 4, and Nanog, and regulated the sphere-forming ability of cells. Additionally, the overexpression or knockdown of lncRNA RUNX1-IT1 regulated the invasion ability of cells by affecting expressions of E-cadherin, N-cadherin, and Vimentin. Conclusions:The poor expression, overexpression, or knockdown of lncRNA RUNX1-IT1 affects the stemness and invasion ability of lung cancer cells.

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Section: Discussionmentioning

confidence: 99%

“…The lncRNA RUNX1-IT1 is a newly discovered non-coding RNA with important functions. The level of expression of lncRNA RUNX1-IT1 in endometrial cancer has been shown to be significantly reduced ( 11 ). Further, the expression of lncRNA RUNX1-IT1 can arrest the growth of liver cancer cells and induce their apoptosis ( 12 ).…”

Section: Introductionmentioning

confidence: 99%