LncRNA-SNHG5 mediates activation of hepatic stellate cells by regulating NF2 and Hippo pathway

Abstract

Long noncoding RNA small nucleolar RNA host gene 5 (SNHG5) is an oncogene found in various human cancers. However, it is unclear what role SNHG5 plays in activating hepatic stellate cells (HSCs) and liver fibrosis. In this study, SNHG5 was found to be upregulated in activated HSCs in vitro and in primary HSCs isolated from fibrotic liver in vivo, and inhibition of SNHG5 suppressed HSC activation. Notably, Neurofibromin 2 (NF2), the main activator for Hippo signalling, was involved in the effects of SNHG5 on HSC activation. The interaction between SNHG5 and NF2 protein was further confirmed, and preventing the combination of the two could effectively block the effects of SNHG5 inhibition on EMT process and Hippo signaling. Additionally, higher SNHG5 was found in chronic hepatitis B patients and associated with the fibrosis stage. Altogether, we demonstrate that SNHG5 could serve as an activated HSCs regulator via regulating NF2 and Hippo pathway.

Proteome Analysis Related to Unsaturated Fatty Acid Synthesis by Interfering with Bovine Adipocyte ACSL1 Gene

“…However, it is noteworthy that the protein levels and mRNA expression do not always exhibit a direct correlation [ 31 , 32 ], with proteins also providing valuable insights into post-translational modifications [ 33 , 34 ]. Additionally, lncRNAs regulate gene expression at the transcriptional and post-transcriptional levels [ 35 , 36 ], but they also regulate the transcription of downstream molecules by binding to proteins and localizing protein complexes to specific DNA sequences [ 37 , 38 ]. In this study, label-free proteomics analysis was performed using RNA interference and non-interference with ACSL1 in bovine adipocytes ( Figure 1 ).…”