lncRNA TSPEAR-AS2, a Novel Prognostic Biomarker, Promotes Oral Squamous Cell Carcinoma Progression by Upregulating PPM1A via Sponging miR-487a-3p

Abstract

Background. Long noncoding RNA (lncRNA) critically impacts the modulation of tumor developments and progressions. Our study is aimed at investigating the expressing patterns, clinical significance, and biological roles of lncRNA TSPEAR-AS2 (TSPEAR-AS2) in oral squamous cell carcinoma (OSCC). Material and Approach. The expressing states achieved by TSPEAR-AS2 were examined in OSCC specimens and cell lines by RT-PCR. The clinical significance of TSPEAR-AS2 was statistically analyzed. OSCC proliferating, invading, and migrating processes were examined with the use of wound healing assays, transwell, colony formation, and cell counting kit-8. Additionally, the downstream molecular mechanism of TSPEAR-AS2 in OSCC was explored. Results. TSPEAR-AS2 was overexpressed in OSCC tumors and cells. High TSPEAR-AS2 was associated with advanced TNM stage. Patients with high TSPEAR-AS2 expression displayed a shorter disease-free survival and total survival of OSCC patients than those with low TSPEAR-AS2 expressing level. It was found that knockdown of TSPEAR-AS2 could inhibit the proliferating, invading, and migrating processes pertaining to OSCC cells. Luciferase reporter tests and RNA pull-down results revealed that TSPEAR-AS2 enhanced the expressions of PPM1A by regulating miR-487a-3p, and TSPEAR-AS2 could be adopted as a miR-487a-3p sponge to inhibit PPM1A expression. Conclusion. Our study highlighted the significance of the TSPEAR-AS2/miR-487a-3p/PPM1A axis within OSCC progression and offered a novel biomarker and novel strategies for OSCC treatments.

Relationship between M6A methylation regulator and prognosis in patients with hepatocellular carcinoma after transcatheter arterial chemoembolization

“…Except for TSPEAR-AS2, which was not controlled, we discovered that six lncRNAs (HCG18, LINC01703, NUTM2A-AS1, SNHG16, TSPEAR-AS2, and THUMPD3-AS1) were considerably up-regulated in hepatocellular carcinoma. Other investigations have discovered that HCG18 stimulates hepatocellular carcinoma advancement through the miR-214-3p/CENPM axis and the circulating D1-WNT pathway in head and neck squamous cell carcinoma [ 25 , 26 ] Similarly, LINC01703 enhances the invasiveness of NSCLC cells by altering Mir-605-3p/MACC1 [ 27 ], LncRNA SNHG16 drives the proliferation and invasion of papillary thyroid carcinoma by regulating Mir-497 [ 28 ], TSPEAR-AS2, Up-regulation of PPM1A by sponging Mir-487a-3p promotes the progression of oral squamous cell carcinoma [ 29 ]. Furthermore, the HSA-Mir-497-5p-NUTM2A-AS1 axis has been verified in recent studies [ 30 ].…”

Long non-coding RNA TTN-AS1/microRNA-199a-3p/runt-related transcription factor 1 gene axis regulates the progression of oral squamous cell carcinoma

“…Next, our study indicates that TTN-AS1 expression is associated with tumor differentiation, T-stage, and lymph node metastasis, but we did not explore the correlation between TTN-AS1 expression and prognosis of OSCC due to the recently collected tissues. We will keep eyes for these patients to explore the effect on survival in the future, similar to the team of Xia et al [ 47 ]. Moreover, Sarode et al [ 48 ] explored the effect of adipose tissue on the submucosal adipose tissue of the oral cavity, suggesting that the influence of the adipose microenvironment on the treatment of OSCC may be an effective method to combat drug resistance.…”

The role of long noncoding RNAs as regulators of the epithelial–Mesenchymal transition process in oral squamous cell carcinoma cells

“…Higher LINC00958 levels are also present in OSCC tumors wherein they can promote oncogenic processes via the miR-185-5p/YWHAZ axis ( Wang et al, 2020a ). PRM1A is indirectly upregulated by TSPEAR-AS2 via its ability to bind and sequester miR-487a-3p during OSCC progression ( Xia et al, 2021 ).…”