LncRNA TTTY15 knockdown alleviates H2O2-stimulated myocardial cell injury by regulating the miR-98-5p/CRP pathway

Ma, Richard; Gao, Lan; Liu, Yanhong; Du, Pengqiang; Chen, Xiaozhen; Li, Gang

doi:10.1007/s11010-020-03887-4

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…TTTY15 is highly expressed in hypoxia-induced endothelial cell injury and interference with its expression attenuates hypoxia-induced endothelial cell injury [11] . TTTY15 is up-regulated in patients with acute myocardial infarction and hydrogen peroxide-induced myocardial cell injury and knockdown of its expression reduces hydrogen peroxide-induced cardiomyocyte injury [12] . Inhibition of TTTY15 expression reduces hypoxia-induced cardiomyocyte injury [13] .…”

Section: Resultsmentioning

confidence: 99%

Study on Mechanism of Long Non-Coding RNA TTTY15 Targeting MicroRNA-942-5p Mediating High Glucose-Induced Renal Tubular Epithelial Cell Injury

Ma¹,

Qian²,

Luo³

et al. 2022

IJPS

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To investigate the effect of long non-coding RNA TTTY15 on high glucose-induced renal tubular epithelial cell injury and its possible mechanism. Human renal tubular epithelial cells HK-2 were induced by high glucose to establish cell injury model. Small molecule interfering-negative control, small molecule interfering-RNA gene TTTY15, microRNA-negative control, miR-942-5p mimics, small molecule interfering-RNA gene TTTY15 and anti-microRNA-negative control, small molecule interfering-RNA gene TTTY15 and anti-miR-942-5p were transfected into HK-2 cells by liposome transfection method and then treated with 25 mmol/l glucose for 24 h. The expression of TTTY15 and microRNA-942-5p was detected by quantitative real time polymerase chain reaction; the levels of interleukin-6 and tumor necrosis factor-alpha in the supernatant were detected by enzyme-linked immunosorbent assay; apoptosis rate was detected by flow cytometry. Dual-luciferase report assay verified the targeted regulation relationship between TTTY15 and microRNA-942-5p; the expression of cleaved caspase-3 and cleaved caspase-9 proteins was detected by Western blot. The expression of TTTY15 increased (p<0.05) and microRNA-942-5p decreased (p<0.05) in HK-2 cells induced by high glucose; transfection of small molecule interfering-RNA gene TTTY15 or microRNA-942-5p mimics could decrease the levels of interleukin-6, tumor necrosis factor-alpha (p<0.05), apoptosis, cleaved caspase-3 and cleaved caspase-9 proteins in HK-2 cells induced by high glucose (p<0.05); TTTY15 can regulate the expression of microRNA-942-5p; co-transfection of small molecule interfering-RNA gene TTTY15 and anti-miR-942-5p decreased the effect of small molecule interfering-RNA gene TTTY15 on inflammatory reaction and apoptosis induced by high glucose in HK-2 cells. Interference with TTTY15 expression may attenuate high glucose-induced tubular epithelial cell injury by targeting microRNA-942-5p expression and inhibiting the inflammatory reaction and apoptosis.

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Section: Resultsmentioning

confidence: 99%

Study on Mechanism of Long Non-Coding RNA TTTY15 Targeting MicroRNA-942-5p Mediating High Glucose-Induced Renal Tubular Epithelial Cell Injury

Ma¹,

Qian²,

Luo³

et al. 2022

IJPS

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show abstract

“…Previous studies have shown that TTTY15 is implicated in the regulation of a series of physiological and pathological processes. For example, knocking down TTTY15 reduces H 2 O 2 -induced cardiomyocyte injury via modulating the miR-98-5p/CRP axis [ 29 ]; TTTY15 can promote hypoxia-induced vascular endothelial cell injury by down-regulating miR-186-5p [ 30 ]. Importantly, TTTY15 is also involved in regulating the proliferation, differentiation, apoptosis and other biological processes of cancer cells [ 9 , 11 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p

Zheng

Peng

Wu³

et al. 2022

Bioengineered

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The present study is aimed to investigate the regulatory effects and related mechanism of long non-coding RNA testis-specific transcript, Y-linked 15 (TTTY15) in gastric carcinoma (GC) cell proliferation, migration, invasion, apoptosis and epithelial–mesenchymal transition (EMT). TTTY15 expression in GC tissue samples and cells was detected by quantitative real-time PCR (qRT-PCR), and the correlation between TTTY15 expression and GC clinicopathological indicators was analyzed. Cell counting kit-8 (CCK-8), BrdU, flow cytometry and Transwell assays were performed for detecting GC cell proliferation, migration, invasion and apoptosis. Western blot was performed for detecting the expressions of EMT-associated proteins (N-cadherin and E-cadherin), Wnt family member 1 (Wnt1) protein and β-catenin protein. Bioinformatics analysis was conducted to predict, and RNA immunoprecipitation (RIP) assay and dual-luciferase reporter gene assay were performed to verify the targeted relationships of microRNA let-7a-5p (let-7a-5p) with TTTY15 and Wnt1 mRNA 3'UTR. It was found that TTTY15 expression was significantly up-regulated in GC tissues and cells, and was associated with advanced TNM stage and poor tumor differentiation. TTTY15 overexpression promoted GC cell proliferation, migration and invasion, the expressions of N-cadherin, Wnt1 and β-catenin protein, and inhibited the apoptosis and E-cadherin expression, while knocking down TTTY15 had the opposite effects. TTTY15 directly targeted let-7a-5p and negatively regulated its expression. Wnt1 was the target gene of let-7a-5p, and TTTY15 could indirectly and positively regulate Wnt1 expression. In conclusion, TTTY15 promotes GC progression, by regulating the let-7a-5p/Wnt1 axis to activate the Wnt/β-catenin pathway.

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“…It has been demonstrated that TTTY15 plays an important role in regulating hypoxia-induced vascular endothelial cell injury [48]. Knockdown of long noncoding RNA TTTY15 helps protect cardiomyocytes in case of hypoxia-induced apoptosis and 10 BioMed Research International mitochondrial energy metabolism dysfunction via TTTY15/ let-7i-5p and TLR3/NF-κB pathways [49]; and TTTY15 knockdown also protects cardiomyocytes from hypoxiainduced injury by regulating the let-7b/MAPK6 axis [50] or by regulating the miR-98-5p/CRP pathway [51]. Moreover, the polymorphism of ARHGAP9 is associated with coronary artery spasm [38].…”

Section: Discussionmentioning

confidence: 99%

Analyses of Long Noncoding RNA and mRNA Profiles in Subjects with the Phlegm-Dampness Constitution

Dong

Zheng

Liú

et al. 2021

BioMed Research International

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Background. Constitution in traditional Chinese medicine (TCM) plays a key role in the genesis, development, and prognosis of diseases. Phlegm-dampness constitution (PDC) is one of the nine constitutions in TCM, susceptible to metabolic disorders, which is mainly manifested by profuse phlegm, loose abdomen, and greasy face. Epidemiologic, genomic, and epigenetic studies have been carried out in previous works, confirming that PDC represents a distinctive population with microcosmic changes related to metabolic disorders. However, whether long noncoding RNAs (lncRNAs) play a regulatory role in metabolic disease in subjects with PDC remains largely unknown. We aimed to investigate distinct lncRNA and mRNA expression signatures and lncRNA-mRNA regulatory networks in the phlegm-dampness constitution (PDC). Methods. The peripheral blood mononuclear cells (PBMCs) were isolated from the subjects with PDC ( n = 13 ) and balanced constitution (BC) ( n = 9 ). The profiles of lncRNAs and mRNAs in PBMCs were analyzed using microarray and further validated with RT-qPCR. Subsequently, pathway analysis was performed to investigate the function of differentially expressed mRNAs by using Ingenuity Pathway Analysis (IPA). Results. Results suggested that some mRNAs, which were regulated by the differentially expressed lncRNAs, were mainly enriched in lipid metabolism and immune inflammation-related pathways. This was consistent with the molecular characteristics of previous studies, indicating that the clinical characteristics of metabolic disorders in PDC might be regulated by lncRNAs. Furthermore, by making coexpression network construction as well as cis-regulated target gene analysis, several lncRNA-mRNA pairs with potential regulatory relationships were identified by bioinformatic analyses, including RP11-317J10.2-CA3, RP11-809C18.3-PIP4K2A, LINC0069-RFTN1, TTTY15-ARHGEF9, and AC135048.13-ORAI3. Conclusions. This study first revealed that the expression characteristics of lncRNAs/mRNAs may be potential biomarkers, indicating that the distinctive physical and clinical characteristics of PDC might be partially attributed to the specific expression signatures of lncRNAs/mRNAs.

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LncRNA TTTY15 knockdown alleviates H2O2-stimulated myocardial cell injury by regulating the miR-98-5p/CRP pathway

Cited by 14 publications

References 23 publications

Study on Mechanism of Long Non-Coding RNA TTTY15 Targeting MicroRNA-942-5p Mediating High Glucose-Induced Renal Tubular Epithelial Cell Injury

Study on Mechanism of Long Non-Coding RNA TTTY15 Targeting MicroRNA-942-5p Mediating High Glucose-Induced Renal Tubular Epithelial Cell Injury

Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p

Analyses of Long Noncoding RNA and mRNA Profiles in Subjects with the Phlegm-Dampness Constitution

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