LncRNA TUG1 promoted osteogenic differentiation through promoting bFGF ubiquitination

Yu, Yang; Chen, Ying; Zheng, Yijing; Weng, Qihao; Zhu, Sipin; Zhou, Dongsheng

doi:10.1007/s11626-019-00410-y

Cited by 20 publications

(11 citation statements)

References 30 publications

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“… 17 LncRNA TUG1 regulates osteogenic differentiation of TSPCs by promoting bFGF ubiquitination. 43 Furthermore, lncRNA H19 accelerates tenogenic differentiation of TDSCs and promotes tendon healing via modulation of miR-29b-3p-regulated TGF-b1 expression. 18 Further, prior studies suggest that H19 has potential roles in regulating mammalian muscle growth and development.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling

et al. 2021

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Rotator cuff tear (RCT) is a common tendon injury, but the mechanisms of tendon healing remain incompletely understood. Elucidating the molecular mechanisms of tenogenic differentiation is essential to develop novel therapeutic strategies in clinical treatment of RCT. The long noncoding RNA H19 plays a regulatory role in tenogenic differentiation and tendon healing, but its detailed mechanism of action remains unknown. To elucidate the role of H19 in tenogenic differentiation and tendon healing, tendon-derived stem cells were harvested from the Achilles tendons of Sprague Dawley rats and a rat model of cuff tear was established for the exploration of the function of H19 in promoting tenogenic differentiation. The results showed that H19 overexpression promoted, while H19 silencing suppressed, tenogenic differentiation of tendon-derived stem cells (TDSCs). Furthermore, bioinformatic analyses and a luciferase reporter gene assay showed that H19 directly targeted and inhibited miR-140-5p to promote tenogenic differentiation. Further, inhibiting miR-140-5p directly increased VEGFA expression, revealing a novel regulatory axis between H19, miR-140-5p, and VEGFA in modulating tenogenic differentiation. In rats with RTC, implantation of H19-overexpressing TDSCs at the lesion promoted tendon healing and functional recovery. In general, the data suggest that H19 promotes tenogenic differentiation and tendon-bone healing by targeting miR-140-5p and increasing VEGFA levels. Modulation of the H19/miR-140-5p/VEGFA axis in TDSCs is a new potential strategy for clinical treatment of tendon injury.

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling

et al. 2021

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“…For a deeper understanding, we also explored the possible mechanoresponsive and osteogenic pathways that these lncRNAs may participate in. H19, KCNQ1OT1, TUG1, SNHG1, and MALAT1, highlighted in our network, are closely correlated with osteogenesis via the ceRNA mechanism and beyond, which mainly involves BMP/Smad, RUNX2, MAPK, and Wnt/ β -catenin signaling pathways ( Yu C. et al, 2018 ; Gong et al, 2018 ; He et al, 2018 ; Gu H. et al, 2019 ; Liu H. et al, 2019 ; Wang C.-G. et al, 2019 ; Wu et al, 2019a ; Jiang et al, 2019 ; Wu D. et al, 2020 ; Bi et al, 2020 ; Wang J. L et al, 2020 ; Xiang et al, 2020 ; Xiaoling et al, 2020 ; Yu et al, 2020 ), and detailed relationships are concluded in Table 4 .…”

Section: Resultsmentioning

confidence: 85%

A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling

Cai

et al. 2022

Front. Bioeng. Biotechnol.

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Mechanical force, being so ubiquitous that it is often taken for granted and overlooked, is now gaining the spotlight for reams of evidence corroborating their crucial roles in the living body. The bone, particularly, experiences manifold extraneous force like strain and compression, as well as intrinsic cues like fluid shear stress and physical properties of the microenvironment. Though sparkled in diversified background, long noncoding RNAs (lncRNAs) concerning the mechanotransduction process that bone undergoes are not yet detailed in a systematic way. Our principal goal in this research is to highlight the potential lncRNA-focused mechanical signaling systems which may be adapted by bone-related cells for biophysical environment response. Based on credible lists of force-sensitive mRNAs and miRNAs, we constructed a force-responsive competing endogenous RNA network for lncRNA identification. To elucidate the underlying mechanism, we then illustrated the possible crosstalk between lncRNAs and mRNAs as well as transcriptional factors and mapped lncRNAs to known signaling pathways involved in bone remodeling and mechanotransduction. Last, we developed combinative analysis between predicted and established lncRNAs, constructing a pathway–lncRNA network which suggests interactive relationships and new roles of known factors such as H19. In conclusion, our work provided a systematic quartet network analysis, uncovered candidate force-related lncRNAs, and highlighted both the upstream and downstream processes that are possibly involved. A new mode of bioinformatic analysis integrating sequencing data, literature retrieval, and computational algorithm was also introduced. Hopefully, our work would provide a moment of clarity against the multiplicity and complexity of the lncRNA world confronting mechanical input.

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“…For example, knockdown of NUTM2B-AS1, which was recently shown to be involved in a neuromuscular disorder, 36 was enriched in the neuron cell-type. Knockdown of TUG1 reflects negative enrichment of osteoblast, tendon and fat cells, with TUG1 promoting osteogenic differentiation from periodontal ligament stem cells 37 and tendon stem cells, 38 as well as promoting brown remodeling of white adipose tissue. 33 To validate the role of lncRNAs which modulate stem-cell identity, we performed a colony formation assay with lncRNA knockdown.…”

Section: Lncrna Modulates Cell Identitiesmentioning

confidence: 99%

Antisense oligonucleotide-mediated perturbation of long non-coding RNA reveals functional features in stem cells and across cell types

Yip

Hon

Yasuzawa

et al. 2022

Preprint

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Long non-coding RNAs (lncRNAs) were shown to regulate pluripotency and differentiation, however, a comprehensive assessment of their regulatory roles in stem cells is currently lacking. By performing a large-scale knockdown of lncRNAs (n = 390) in human induced pluripotent stem (iPS) cells, we systematically characterized their roles in self-renewal and pluripotency. Cell growth and transcriptomic assays revealed respectively, 18.3% and 29.3% of these lncRNAs in altering cellular and molecular phenotypes upon knockdown. By integrating the molecular phenotypes with chromatin-interaction assays, we identified their cis- and trans- interacting partners as potential primary targets. In addition, cell-type enrichment revealed 16 lncRNAs associated with pluripotency and we highlighted LINC02595, CATG00000090305.1 and RP11-148B6.2. Next, we integrated fibroblasts phenotyping data (Ramilowski et al., 2020; Genome Research) and reported that only 2.9% lncRNAs exhibited consistent cell growth phenotype, while molecular phenotype showed 66.7% agreement. This highlights their consistent primary functional roles across cell-types and the distinct secondary effects under different cellular environments.

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LncRNA TUG1 promoted osteogenic differentiation through promoting bFGF ubiquitination

Cited by 20 publications

References 30 publications

Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling

Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling

A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling

Antisense oligonucleotide-mediated perturbation of long non-coding RNA reveals functional features in stem cells and across cell types

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