lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer

Li, Chengren; Li, Xu; Liu, Yongguang

doi:10.1186/s12957-022-02529-x

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…It is reported that USP30-AS1 in our model also participated in the other immune-related lncRNA model based on TCGA and GTEx database, implying the importance of USP30-AS1 in OC [48]. Besides, several studies reported the role of USP30-AS1 in carcinogenesis [48][49][50][51][52]. But there were no studies of USP30-AS1 in OC, which needed to be future explored.…”

Section: Discussionmentioning

confidence: 88%

An Immune‐Related lncRNA Pairing Model for Predicting the Prognosis and Immune‐Infiltrating Cell Condition in Human Ovarian Cancer

Zhang

Yang

2022

BioMed Research International

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Ovarian cancer is the second common cancer among the gynecological tumors. It is difficult to be found and diagnosed in the early stage and easy to relapse due to chemoresistance and deficiency in choices of treatment. Therefore, future exploring the biomarkers for diagnosis, treatment, and prognosis prediction of ovarian cancer is significant to women in the world. We downloaded data from TCGA and GTEx and used R “limma” package for analyzing the differentially expressed immune-related lncRNA in ovarian cancer and finally got 7 downregulated and 171 upregulated lncRNA. Then, we paired the differentially expressed immune-related lncRNA and constructed a novel lncRNA pairing model containing 7 lncRNA pairs. Based on the cut-off point with the highest AUC value, 102 patients were selected in high-risk group and 272 in low-risk group. The KM analysis suggested that the patients in the low-risk group had a longer overall survival. Future analysis showed the correlations between risk scores and clinicopathological parameters and infiltrating immune cells. In conclusion, we identified an immune-related lncRNA pairing model for predicting the prognosis and immune-infiltrating cell condition in human ovarian cancer, which thus further can instruct immunotherapy.

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Section: Discussionmentioning

confidence: 88%

An Immune‐Related lncRNA Pairing Model for Predicting the Prognosis and Immune‐Infiltrating Cell Condition in Human Ovarian Cancer

Zhang

Yang

2022

BioMed Research International

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“…Although some differentially expressed genes were shared between T2D and control samples ( Figure 3 B), 181 up- and 179 down-regulated genes were differentially regulated specifically in T2D compared to the control samples, including 35 up- and 22 down-regulated lncRNA genes ( Supplementary Table S6 ). Among these up-regulated lncRNA genes, only the following nine have been previously studied: (1) ENSG00000232855 (novel transcript) [ 75 ]; (2) ENSG00000272512 (novel transcript) [ 76 ]; (3) KCNJ2 antisense RNA 1 (KCNJ2-AS1) [ 77 , 78 , 79 ]; (4) KLHDC7B divergent transcript (KLHDC7B-DT) [ 80 , 81 , 82 ]; (5) long intergenic non-protein-coding RNA 2015 (LINC02015) [ 83 , 84 ]; (6) long intergenic non-protein-coding RNA 2381 (LINC02381) [ 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 ]; (7) long intergenic non-protein-coding RNA 2541 (LINC02541) [ 94 ]; (8) serpin family B member 9 pseudogene 1 (SERPINB9P1) [ 95 , 96 , 97 ]; and (9) USP30 antisense RNA 1 (USP30-AS1) [ 98 , 99 , 100 ]. However, only the functions of the lncRNA genes LINC02381 and USP30_AS1 are known, with the former known to bind transcription factors [ 85 ], RNA-binding proteins [ 86 ], and miRNAs [ 87 , 88 , 89 , 90 , 91 , 92 , 93 ]; while USP30-AS1 acts as a miRNA sponge [ 98 , 99 ] and is involved in the cis-regulation of the nearby protein-coding genes USP30 and ANKRD13A [ 100 ].…”

Section: Resultsmentioning

confidence: 99%

T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes

Distefano

Ilieva

Madsen

et al. 2023

ncRNA

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Type II diabetes (T2D) is a growing health problem worldwide due to increased levels of obesity and can lead to other life-threatening diseases, such as cardiovascular and kidney diseases. As the number of individuals diagnosed with T2D rises, there is an urgent need to understand the pathogenesis of the disease in order to prevent further harm to the body caused by elevated blood glucose levels. Recent advances in long non-coding RNA (lncRNA) research may provide insights into the pathogenesis of T2D. Although lncRNAs can be readily detected in RNA sequencing (RNA-seq) data, most published datasets of T2D patients compared to healthy donors focus only on protein-coding genes, leaving lncRNAs to be undiscovered and understudied. To address this knowledge gap, we performed a secondary analysis of published RNA-seq data of T2D patients and of patients with related health complications to systematically analyze the expression changes of lncRNA genes in relation to the protein-coding genes. Since immune cells play important roles in T2D, we conducted loss-of-function experiments to provide functional data on the T2D-related lncRNA USP30-AS1, using an in vitro model of pro-inflammatory macrophage activation. To facilitate lncRNA research in T2D, we developed a web application, T2DB, to provide a one-stop-shop for expression profiling of protein-coding and lncRNA genes in T2D patients compared to healthy donors or subjects without T2D.

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“…For instance, aspirin‐regulated lncRNA NEAT1 and LOC152578 may contribute to suppressed cell growth and metastasis in colon cancer 44 . Additionally, USP30‐AS1 was reported to repress the development of colon cancer by regulating miR‐765 45 . It is noteworthy that few studies have uncovered the involvement of MIR143HG in colon cancer.…”

Section: Discussionmentioning

confidence: 99%

“…44 Additionally, USP30-AS1 was reported to repress the development of colon cancer by regulating miR-765. 45 It is noteworthy that few studies have uncovered the involvement of MIR143HG in colon cancer. Intriguingly, prior has suggested that overexpression of may lead to glioblastoma cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

MIR143HG promotes methylation of transcription factor HOXB7 promoter by recruiting methyltransferase DNMT1 to prevent the progression of colon cancer

Zhu,

Xu,

et al. 2023

The FASEB Journal

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In recent years, accumulating evidence has demonstrated the role of long noncoding RNAs (lncRNAs) in colon cancer. We aim to investigate the role of MIR143HG, also known as CARMN (Cardiac mesoderm enhancer‐associated noncoding RNA) in colon cancer and explore the related mechanisms. An RNAseq data analysis was performed to screen differentially expressed lncRNAs associated with colon cancer. Next, MIR143HG expression was quantified in colon cancer cells. Moreover, the contributory roles of MIR143HG in the progression of colon cancer with the involvement of DNMT1 and HOXB7 (Homeobox B7) were evaluated after restored MIR143HG or depleted HOXB7. Finally, the effects of MIR143HG were investigated in vivo by measuring tumor formation in nude mice. High‐throughput transcriptome sequencing was employed to validate the specific mechanisms by which MIR143HG and HOXB7 affect tumor growth in vivo. MIR143HG was found to be poorly expressed, while HOXB7 was highly expressed in colon cancer. MIR143HG could promote HOXB7 methylation by recruiting DNMT1 to reduce HOXB7 expression. Upregulation of MIR143HG or downregulation of HOXB7 inhibited cell proliferation, invasion and migration and facilitated apoptosis in colon cancer cells so as to delay the progression of colon cancer. The same trend was identified in vivo. Our study provides evidence that restoration of MIR143HG suppressed the progression of colon cancer via downregulation of HOXB7 through DNMT1‐mediated HOXB7 promoter methylation. Thus, MIR143HG may be a potential candidate for the treatment of colon cancer.

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lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer

Cited by 14 publications

References 27 publications

An Immune‐Related lncRNA Pairing Model for Predicting the Prognosis and Immune‐Infiltrating Cell Condition in Human Ovarian Cancer

An Immune‐Related lncRNA Pairing Model for Predicting the Prognosis and Immune‐Infiltrating Cell Condition in Human Ovarian Cancer

T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes

MIR143HG promotes methylation of transcription factor HOXB7 promoter by recruiting methyltransferase DNMT1 to prevent the progression of colon cancer

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