LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR‐15b in the endometrial epithelium cells of dairy goat

Abstract

Despite the fact that long noncoding RNAs (lncRNAs) play roles in almost all biological processes, little is known about their biological function in the endometrium during the formation of endometrial receptivity. In this study, a comprehensive analysis of lncRNAs in goat endometrial tissues on Day 5 (prereceptive endometrium, PE) and Day 15 (receptive endometrium, RE) of pregnancy was performed by using RNA-Seq. As a result, 668 differentially expressed lncRNAs (DELs) were found between the PE and RE. Further study showed that lncRNA882, regulated by estrogen (E2) and progestin (P4), could act as competing endogenous RNAs (ceRNAs) for miR-15b, which inhibited the expression of transforming growth factor-b-activated kinase 1 binding protein 3 (TAB3) and then indirectly regulated the level of leukemia inhibitory factor (LIF). This was helpful for the formation of endometrial receptivity in dairy goats. In conclusion, we elucidated the endometrium lncRNA profiles of PE and RE in dairy goats; lncRNA882 acted as a ceRNA for miR-15b and then indirectly regulated the level of LIF in goat endometrial epithelium cells. Thus, this study helped us to better understand the molecular regulation of endometrial receptivity in dairy goats. K E Y W O R D Sleukemia inhibitory factor (LIF), endometrial epithelium cells (EECs), long noncoding RNA 882 (lncRNA882), miR-15b

The Complicated Effects of Extracellular Vesicles and Their Cargos on Embryo Implantation

“…LIF is a kind of cytokine secreted by endometrial glands and plays an amazingly important role in embryo implantation during WOI (93,94). As a competing endogenous RNA for miR-15b, lncRNA882 regulates LIF by sponging miR-15b in endometrial epithelium cells of dairy goat (95). (There are 3 EVs-related references in this section.…”

Identification of a potential prognostic lncRNA‐miRNA‐mRNA signature in endometrial cancer based on the competing endogenous RNA network

“…Zhou et al showed that the lncRNA regulator of reprogramming could act as a ceRNA and “sponge” miR‐145 to inhibit its mediation of endometrial cancer stem cell differentiation. Besides this, Zhang et al elucidated that lncRNA882 regulated leukemia inhibitory factor by sponging miR‐15b. However, their samples were mainly collected from goat and only a few correlation tests were carried out …”

“…Besides this, Zhang et al elucidated that lncRNA882 regulated leukemia inhibitory factor by sponging miR‐15b. However, their samples were mainly collected from goat and only a few correlation tests were carried out …”

“…However, their samples were mainly collected from goat and only a few correlation tests were carried out. 15 Given the lack of comprehensive investigations on ceRNAs in endometrial cancer, we used available ncRNA and mRNA expression and single-nucleotide polymorphism array data of UCEC from The Cancer Genome Atlas (TCGA) and constructed a ceRNA-ceRNA interaction network for endometrial cancer. We were mainly interested in the ceRNAs involved in cancer processes.…”

A Novel Molecule in Human Cyclic Endometrium: LncRNA TUNAR Is Involved in Embryo Implantation

“…The expression of H19 was down-regulated in the endometrium of RIF (Zeng et al, 2017). LncRNA882 acted as a ceRNA for miR-15b and then indirectly regulated the level of LIF in goat endometrial epithelial cells (EECs) (Zhang et al, 2019).…”