LncRNAs: Bridging environmental sensing and gene expression

Beck, Zachary T.; Zheng, Xing; Tran, Elizabeth

doi:10.1080/15476286.2016.1240139

Cited by 20 publications

(20 citation statements)

References 109 publications

(138 reference statements)

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“…In agreement with this notion, multiple reports have shown that plant ncRNAs including antisense transcripts are extensively regulated by external cues [30,32,37]. Antisense transcription has been implicated in the regulation of gene expression in response to environmental cues in other eukaryotes including yeast and mammals [38,72,73]. However, in contrast to FLC and examples from yeast, DOG1 antisense not only facilitates the environmental response, but seems to be absolutely required for it (Fig 3).…”

Section: Embo Reportsmentioning

confidence: 77%

Antisense transcription represses Arabidopsis seed dormancy QTL DOG 1 to regulate drought tolerance

et al. 2017

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Plants have developed multiple strategies to sense the external environment and to adapt growth accordingly. () is a major quantitative trait locus (QTL) for seed dormancy strength in that is reported to be expressed exclusively in seeds. is extensively regulated, with an antisense transcript () suppressing its expression in seeds. Here, we show that shows high levels in mature plants where it suppresses expression under standard growth conditions. Suppression is released by shutting down antisense transcription, which is induced by the plant hormone abscisic acid (ABA) and drought. Loss of results in constitutive high-level expression, conferring increased drought tolerance, while inactivation of causes enhanced drought sensitivity. The unexpected role of in environmental adaptation of mature plants is separate from its function in seed dormancy regulation. The requirement of to respond to ABA and drought demonstrates that antisense transcription is important for sensing and responding to environmental changes in plants.

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Section: Embo Reportsmentioning

confidence: 77%

Antisense transcription represses Arabidopsis seed dormancy QTL DOG 1 to regulate drought tolerance

et al. 2017

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“…Long noncoding RNAs (lncRNAs) are a class of noncoding RNAs with lengths exceeding 200 nucleotides (nt). LncRNAs contribute to transcriptional and post-transcriptional functions 11 and can broadly be classified as signaling molecules, decoy molecules, guide molecules, or scaffold molecules 12 . Abundant evidence has confirmed that lncRNAs are involved in multiple tumorigenic and oncogenic processes 13 .…”

Section: Introductionmentioning

confidence: 99%

LINC01016 promotes the malignant phenotype of endometrial cancer cells by regulating the miR-302a-3p/miR-3130-3p/NFYA/SATB1 axis

Pan

Huo

et al. 2018

Cell Death Dis

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Long noncoding RNAs (lncRNAs) have been implicated in tumorigenesis and cancer progression and are tightly associated with the phenotypes of numerous cancers. However, the functional roles underlying these effects are unknown. The expression levels of LINC01016, miR-302a-3p, miR-3130-3p, NFYA, and SATB1 were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in 33 endometrial cancer tissues and 20 normal tissues. Bioinformatics analyses, luciferase reporter analyses, chromatin immunoprecipitation (ChIP) assays, and qRT-PCR assays were performed to verify potential binding sites. The qRT-PCR and western blot were used to identify the regulatory mechanisms of LINC01016 in cell biological behavior, which were also examined by cell counting kit -8 (CCK-8), 5-ethynyl-2′-deoxyuridine (EdU) assays, flow cytometry, wound healing assays, and transwell assays. LINC01016 was substantially upregulated in endometrial cancer tissues, and LINC01016 silencing abolished the malignant behavior of endometrial cancer cells. LINC01016 positively rescued the downstream gene nuclear factor YA (NFYA) by competitively “sponging” miR-302a-3p and miR-3130-3p. In turn, these two miRNAs could inhibit LINC01016 transcription, thus forming two reciprocal repression cycles, which influenced the biological behavior of endometrial cancer cells. MiR-302a-3p and miR-3130-3p could specifically bind with the 3′-UTR regions of NFYA, and NFYA could upregulate the expression of special AT-rich sequence-binding protein 1 (SATB1) as a transcriptional factor. This study was the first to show that the LINC01016–miR-302a-3p/miR-3130-3p/NFYA/SATB1 axis played a crucial role in the occurrence of endometrial cancer. These findings may provide relevant insights into the diagnosis and therapy of endometrial cancer.

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“…Recently, the roles of long noncoding RNAs (lncRNAs) in cancer progression have been intensively investigated due to the diverse cellular functions (cell growth, metastasis, apoptosis, differentiation) of lncRNAs. 6 , 7 The lncRNAs are a class of endogeneous RNA with more than 200 nucleotides in length and cannot code proteins. 8 The dysregulation of lncRNAs has been implicated in various types of cancers.…”

Section: Introductionmentioning

confidence: 99%

The lncRNA PCAT1 is correlated with poor prognosis and promotes cell proliferation, invasion, migration and EMT in osteosarcoma

Zhang¹,

Zhang²,

Mao³

et al. 2018

OTT

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IntroductionOsteosarcoma is a malignant primary bone cancer and is lethal to children and adolescents. Recently, the dysregulation of long noncoding RNAs (lncRNAs) has been shown in various types of cancers.AimThe present study aimed to examine the role of the lncRNA prostate cancer-associated transcript 1 (PCAT1) in osteosarcoma progression.Materials and methodsThe expression levels of relevant genes in clinical samples and cell lines were determined by quantitative real-time polymerase chain reaction. Cell proliferation, invasion and migration were examined by CCK-8 assay, transwell invasion and migration assay, respectively. Cell apoptosis and cell cycle were detected by flow cytometry. Protein levels were detected by Western blot.ResultsOur results showed that PCAT1 was upregulated in osteosarcoma tissues when compared to normal bone tissues. PCAT1 was also upregulated in osteosarcoma cell lines when compared to normal bone cell line. The upregulation of PCAT1 was significantly associated with advanced clinical stage, tumor metastasis and shorter overall survival in patients with osteosarcoma. In vitro studies showed that overexpression of PCAT1 in MG-63 cells enhanced cell proliferation, cell invasion and migration and epithelial-to-mesenchymal transition (EMT); decreased cell apoptotic rate; and also caused an increase in cell population at S phase with a decrease in cell population at G0/G1 phase. Knockdown of PCAT1 in U2OS cells suppressed cell proliferation, cell invasion and migration, and EMT; increased cell apoptotic rate; and caused an increase in the cell population at G0/G1 phase with a decrease in cell population at S phase.ConclusionTaken together, our results suggest the oncogenic role of PCAT1 in osteosarcoma progression.

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LncRNAs: Bridging environmental sensing and gene expression

Cited by 20 publications

References 109 publications

Antisense transcription represses Arabidopsis seed dormancy QTL DOG 1 to regulate drought tolerance

Antisense transcription represses Arabidopsis seed dormancy QTL DOG 1 to regulate drought tolerance

LINC01016 promotes the malignant phenotype of endometrial cancer cells by regulating the miR-302a-3p/miR-3130-3p/NFYA/SATB1 axis

The lncRNA PCAT1 is correlated with poor prognosis and promotes cell proliferation, invasion, migration and EMT in osteosarcoma

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