lncRNAs dysregulation in monocytes from primary antiphospholipid syndrome patients: a bioinformatic and an experimental proof-of-concept approach

Guzmán-Martín, Carlos A.; Juárez-Vicuña, Yaneli; Domínguez‐López, Aarón; González-Ramírez, Javier; Amezcua-Guerra, Luis M.; Martínez-Martínez, Laura Aline; Sánchez‐Muñoz, Fausto

doi:10.1007/s11033-022-08080-y

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…Interestingly, a recent experimental study characterizing the molecular signature of 54 biopsy specimens from lupus nephritis patients demonstrated that the aforementioned lncRNA TP53TG1 inversely correlated with the degree of glomerulosclerosis [55]. Conversely, none of the lncRNAs reported in studies of APS patients correlated with lncRNAs that showed alignment with syncytin-1 or syncytin-2 genes [32,33]. Table 5.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, there are no studies investigating the occurrence of dysregulated synthesis of the HERV-derived env proteins syncytin-1 and syncytin-2 in SLE patients. The role of syncytins in APS pathogenesis also remains unknown, although some recent studies have reported an altered lncRNA signature in these patients compared with controls [32,33]. Indeed, lncRNAs may contribute to several steps of APS pathogenesis, including leukocyte activation, immunothrombosis, and impaired embryonic development [34], but whether these events are influenced by the abnormal expression of syncytin genes has not been investigated to date.…”

Section: Discussionmentioning

confidence: 99%

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Interaction between Long Noncoding RNAs and Syncytin-1/Syncytin-2 Genes and Transcripts: How Noncoding RNAs May Affect Pregnancy in Patients with Systemic Lupus Erythematosus

Talotta

2023

IJMS

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Background: Patients with systemic lupus erythematosus (SLE) often suffer from obstetric complications not necessarily associated with the antiphospholipid syndrome. These events may potentially result from the reduced placental synthesis of the fusogenic proteins syncytin-1 and syncytin-2, observed in women with pregnancy-related disorders. SLE patients have an aberrant noncoding (nc)RNA signature that may in turn dysregulate the expression of syncytin-1 and syncytin-2 during placentation. The aim of this research is to computationally evaluate and characterize the interaction between syncytin-1 and syncytin-2 genes and human ncRNAs and to discuss the potential implications for SLE pregnancy adverse outcomes. Methods: The FASTA sequences of the syncytin-1 and syncytin-2 genes were used as inputs to the Ensembl.org library to find any alignments with human ncRNA genes and their transcripts, which were characterized for their tissue expression, regulatory activity on adjacent genes, biological pathways, and potential association with human disease. Results: BLASTN analysis revealed a total of 100 hits with human long ncRNAs (lncRNAs) for the syncytin-1 and syncytin-2 genes, with median alignment scores of 151 and 66.7, respectively. Only lncRNAs TP53TG1, TTTY14, and ENSG00000273328 were reported to be expressed in placental tissue. Dysregulated expression of lncRNAs TP53TG1, LINC01239, and LINC01320 found in this analysis has previously been described in SLE patients as well as in women with a high-risk pregnancy. In addition, some of the genes adjacent to lncRNAs aligned with syncytin-1 or syncytin-2 in a regulatory region might increase the risk of pregnancy complications or SLE. Conclusions: This is the first computational study showing alignments between syncytin-1 and syncytin-2 genes and human lncRNAs. Whether this mechanism affects syncytiotrophoblast morphogenesis in SLE females is unknown and requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Interaction between Long Noncoding RNAs and Syncytin-1/Syncytin-2 Genes and Transcripts: How Noncoding RNAs May Affect Pregnancy in Patients with Systemic Lupus Erythematosus

Talotta

2023

IJMS

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“…In this line of information, lncRNA HOTAIRM1 has been described to play a critical role in inflammatory and allergic response; one study led by Lihua Li and collaborators found that HOTAIRM1 are implicated in allergic rhinitis by promoting differentiation of T helper type 9 cells through miR-148a-3p interaction [ 23 ]. Similarly, in recent years, MZF1-AS1 has been proposed as a promising actor in different pathological phenotypes, including autoimmunity, cancer, and chronic pulmonary diseases [ 18 , 24 , 25 ]. In sum, these studies suggest that overexpression of OIP5-AS1, GAS5, HOTAIRM1, and MZF1-AS1 play crucial roles in the pathophysiology of several diseases in which more severe states are related; and as we can observe in the bioinformatic interaction pathways image, OIP5-AS1, MZF1-AS1, HOTAIRM1, and GAS5 may indirectly interact with genes and microRNAs that have been described as critical components of the role of low-grade inflammation (i.e., meta inflammation) in some endotypes of asthma, which make us hypothesize that whole blood overexpression of these lncRNAs may be part of a characteristic phenotype of asthmatic patients.…”

Section: Discussionmentioning

confidence: 99%

Whole Blood Expression Levels of Long Noncoding RNAs: HOTAIRM1, GAS5, MZF1-AS1, and OIP5-AS1 as Biomarkers in Adolescents with Obesity-Related Asthma

Leija-Martínez

Guzmán-Martín

González-Ramírez

et al. 2023

IJMS

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Asthma is a heterogeneous entity encompassing distinct endotypes and varying phenotypes, characterized by common clinical manifestations, such as shortness of breath, wheezing, and variable airflow obstruction. Two major asthma endotypes based on molecular patterns are described: type 2 endotype (allergic-asthma) and T2 low endotype (obesity-related asthma). Long noncoding RNAs (lncRNAs) are transcripts of more than 200 nucleotides in length, currently involved in many diverse biological functions, such as chromatin remodeling, gene transcription, protein transport, and microRNA processing. Despite the efforts to accurately classify and discriminate all the asthma endotypes and phenotypes, if long noncoding RNAs could play a role as biomarkers in allergic asthmatic and adolescent obesity-related asthma, adolescents remain unknown. To compare expression levels of lncRNAs: HOTAIRM1, OIP5-AS1, MZF1-AS1, and GAS5 from whole blood of Healthy Adolescents (HA), Obese adolescents (O), allergic asthmatic adolescents (AA) and Obesity-related asthma adolescents (OA). We measured and compared expression levels from the whole blood of the groups mentioned above through RT-q-PCR. We found differentially expressed levels of these lncRNAs between the groups of interest. In addition, we found a discriminative value of previously mentioned lncRNAs between studied groups. Finally, we generated an interaction network through bioinformatics. Expression levels of OIP5-AS1, MZF1-AS1, HOTAIRM1, and GAS5 in whole blood from the healthy adolescent population, obese adolescents, allergic asthma adolescents, and obesity-related asthma adolescents are differently expressed. Moreover, these lncRNAs could act as molecular biomarkers that help to discriminate between all studied groups, probably through molecular mechanisms with several genes and miRNAs implicated.

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“…In recent years, emerging evidence has shed light on the involvement of lncRNAs in keratinocyte biology [ 14 , 15 ]. LncRNAs are transcripts that do not code for proteins of >200 pb in length, once considered transcriptional "noise," are now recognized as key players in gene regulation and have garnered attention for their potential implication in diverse cellular processes [ 16 , 17 ]. Dysregulation of specific lncRNAs can disrupt this balance, leading to abnormal keratinocyte proliferation, impaired differentiation, and compromised barrier integrity, contributing to the pathogenesis of skin disorders such as psoriasis, atopic dermatitis, and skin cancers [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

LncRNA MALAT1 in Keratinocyte function: A review of recent advances

Juárez-Vicuña,

Ruiz-Ojeda,

González-Ramírez

et al. 2024

Non-coding RNA Research

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lncRNAs dysregulation in monocytes from primary antiphospholipid syndrome patients: a bioinformatic and an experimental proof-of-concept approach

Cited by 8 publications

References 16 publications

Interaction between Long Noncoding RNAs and Syncytin-1/Syncytin-2 Genes and Transcripts: How Noncoding RNAs May Affect Pregnancy in Patients with Systemic Lupus Erythematosus

Interaction between Long Noncoding RNAs and Syncytin-1/Syncytin-2 Genes and Transcripts: How Noncoding RNAs May Affect Pregnancy in Patients with Systemic Lupus Erythematosus

Whole Blood Expression Levels of Long Noncoding RNAs: HOTAIRM1, GAS5, MZF1-AS1, and OIP5-AS1 as Biomarkers in Adolescents with Obesity-Related Asthma

LncRNA MALAT1 in Keratinocyte function: A review of recent advances

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