LncRNAs Regulatory Networks in Cellular Senescence

Puvvula, Pavan Kumar

doi:10.3390/ijms20112615

Cited by 85 publications

(66 citation statements)

References 132 publications

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“…We found that Mlinc.28428-positive cells under-expressed H19 and PI16 ( Figure 5A). These genes, that present a diversity of functions, are involved in stress mechanisms (oxydative response and shear stress), inflammation in fibroblasts and MSCs, and senescence pathways [81,82,83,84]. Despite the low number of differentially expressed genes in Mlinc.28428-positive cells, their functional behaviour and their known targets suggest a pathway linked to stress response and senescence establishment that reinforce our previous assumptions on Mlinc.28428 function.…”

Section: The Single Cell Rnaseq: An Emergent Level Of Completion In Msupporting

confidence: 71%

Detailed analysis of public RNAseq data and long non-coding RNA: a proposed enhancement to mesenchymal stem cell characterisation

Riquier

Mathieu

Boureux

et al. 2020

Preprint

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The development of RNA sequencing (RNAseq) and corresponding emergence of public datasets have created new avenues of transcriptional marker search. The long non-coding RNAs (lncRNAs) constitute an emerging class of transcripts with a potential for high tissue specificity and function. Using a dedicated bioinformatics pipeline, we propose to construct a cell-specific catalogue of unannotated lncRNAs and to identify the strongest cell markers. This pipeline uses ab initio transcript identification, pseudoalignment and new methodologies such as a specific k-mer approach for naive quantification of expression in numerous RNAseq data.For an application model, we focused on Mesenchymal Stem Cells (MSCs), a type of adult multipotent stem-cells of diverse tissue origins. Frequently used in clinics, these cells lack extensive characterisation. Our pipeline was able to highlight different lncRNAs with high specificity for MSCs. In silico methodologies for functional prediction demonstrated that each candidate represents one specific state of MSCs biology. Together, these results suggest an approach that can be employed to harness lncRNA as cell marker, showing different candidates as potential actors in MSCs biology, while suggesting promising directions for future experimental investigations.

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Section: The Single Cell Rnaseq: An Emergent Level Of Completion In Msupporting

confidence: 71%

Detailed analysis of public RNAseq data and long non-coding RNA: a proposed enhancement to mesenchymal stem cell characterisation

Riquier

Mathieu

Boureux

et al. 2020

Preprint

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“…There is an increasing body of evidence showing that long noncoding RNAs (lncRNAs) and micro RNAs (miRNAs) are involved in cardiovascular aging. LncRNAs act as key regulators of the central aspects of cardiovascular aging (i.e., cardiac hypertrophy, fibrosis, and endothelial/vascular function), and their role in cardiac biology has been recently reviewed [51][52][53][54][55]. A number of miRNAs have been associated with regulation of senescence in various tissues, including the heart [56,57].…”

Section: Cardiac Senescencementioning

confidence: 99%

Regulatory Mechanisms of Mitochondrial Function and Cardiac Aging

Lin

Kerkelä

2020

IJMS

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Aging is a major risk factor for cardiovascular diseases (CVDs), the major cause of death worldwide. Cardiac myocytes, which hold the most abundant mitochondrial population, are terminally differentiated cells with diminished regenerative capacity in the adult. Cardiomyocyte mitochondrial dysfunction is a characteristic feature of the aging heart and one out of the nine features of cellular aging. Aging and cardiac pathologies are also associated with increased senescence in the heart. However, the cause and consequences of cardiac senescence during aging or in cardiac pathologies are mostly unrecognized. Further, despite recent advancement in anti-senescence therapy, the targeted cell type and the effect on cardiac structure and function have been largely overlooked. The unique cellular composition of the heart, and especially the functional properties of cardiomyocytes, need to be considered when designing therapeutics to target cardiac aging. Here we review recent findings regarding key factors regulating cell senescence, mitochondrial health as well as cardiomyocyte rejuvenation.

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“…It has caused widespread concern that lncRNAs showed a vital role in modulating gene expression at the epigenetic, transcriptional and post-transcriptional levels in various cancers, which became a target of diagnosis, prognosis and therapy in various cancers. 5,6 LncRNAs were also involved in the tumorigenesis of OSCC, mainly by acting as miRNA sponges to down-regulate miRNAs on mRNAs. 7,8 Zinc finger antisense 1 (ZFAS1) is a protooncogene that is aberrantly expressed in diverse cancers, including ovarian cancer, 9 cervical cancer, 10 gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>ZFAS1 Promotes Cisplatin Resistance via Suppressing miR-421 Expression in Oral Squamous Cell Carcinoma</p>

Wang¹,

Hao²,

Wang³

et al. 2020

CMAR

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Oral squamous cell carcinoma (OSCC), with high incidence and mortality, represents one of the main reasons for head and neck malignant tumors. We want to investigate the effect of ZFAS1 on DDP resistance in oral squamous cell carcinoma. Methods: The proliferation and migration of cells was detected by CCK-8 and Transwell assay. The apoptosis was measured by flow cytometry and Western blot. The interaction of ZFAS1, miR-421, and MEIS2 was verified by luciferase reporter assay. The role of ZFAS1 in DDP resistance in vivo was tested by the nude mice model. The expression of ZFAS1 in exosomes from cisplatin-resistant patients was also determined. Results: ZFAS1 overexpression improved OSCC cell growth and inhibited OSCC cell susceptibility to DDP. In addition, the silencing of ZFAS1 promoted DDP-induced apoptosis. ZFAS1 directly bound to miR-421, which was verified by luciferase reporter assay. Inhibition of miR-421 reversed the effect of si-ZFAS1, which promoted the cell viability and decreased the sensitivity of DDP in DDP-resistant cells. The in vivo experiment showed the role of ZFAS1 in increasing the DDP resistance in OSCC tumor. Importantly, this study also showed upregulated ZFAS1 in serum exosomes derived from cisplatin-resistant patients. Conclusion: ZFAS1 promotes chemoresistance of oral squamous cell carcinoma to cisplatin and might become a latent therapeutic target for treating OSCC.

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LncRNAs Regulatory Networks in Cellular Senescence

Cited by 85 publications

References 132 publications

Detailed analysis of public RNAseq data and long non-coding RNA: a proposed enhancement to mesenchymal stem cell characterisation

Detailed analysis of public RNAseq data and long non-coding RNA: a proposed enhancement to mesenchymal stem cell characterisation

Regulatory Mechanisms of Mitochondrial Function and Cardiac Aging

<p>ZFAS1 Promotes Cisplatin Resistance via Suppressing miR-421 Expression in Oral Squamous Cell Carcinoma</p>

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