LncWNT3‐IT affects the proliferation of Sertoli cells by regulating the expression of the WNT3 gene in goat testis

He, Lin; He, Qijie; Qiao, Lei; Huang, Siyi; Dai, Zinuo; Yang, Ting; Liu, Lingbin; Zhao, Zhongquan

doi:10.1111/rda.13738

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…LncRNAs was longer than 200 nt, which could not be translated into any protein, but could regulate the expression of target genes and function in multiple biological processes [ 56 ]. Recently, lncWNT3-IT has been shown to regulate WNT3 expression and affect proliferation of goat SCs, confirming the vital function of lncRNAs in SCs [ 57 ]. In the present study, we identified ten thousands of lncRNAs (most are novel), providing useful information to further elucidate their functions in porcine iSCs.…”

Section: Discussionmentioning

confidence: 91%

Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming

et al. 2020

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Vitamin C (ascorbic acid, AA) can regulate antioxidation and affect many cellular processes. However, the effect of AA on the reproduction of male animals remains less explored. Here, we showed that by supplementing exogenous AA to porcine immature Sertoli cells (iSCs), AA could promote the proliferation, suppress apoptosis, and decrease the global nucleic acid methylation (5 mC and m 6 A) levels of iSCs. After we profiled mRNA and long non-coding RNA (lncRNA) expression by transcriptome sequencing on iSCs (treated by 250 μM AA for 36 h), 1232 mRNAs and 937 lncRNAs were identified to be differentially expressed (DE). Gene enrichment analysis found multiple significantly enriched biological pathways, including oxidoreductase activity, cell proliferation and apoptosis, regulation of hormone level, regulation of catalytic activity, developmental process, ATP metabolism and reproductive process. Specifically, for the reproductive process, 49 up- and 36 down-regulated DE mRNAs (including highly expressed genes, such as Tfcp2l1, Hmgcs1, Mmp7, Fndc3a, and Zfp36l 1) are involved. Moreover, AA supplementation could promote the secretion of anti-müllerian hormone, inhibin B and lactate, and enhance the activity of lactate dehydrogenase as well. Taken together, AA could promote the reproductive function of pig iSCs, potentially through reprogramming the global transcriptome, and elevating hormone secretion and metabolite production.

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Section: Discussionmentioning

confidence: 91%

Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming

et al. 2020

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“…Experimental reduction of the expression of lncRNA H19 significantly down-regulated the expression levels of IGF-1R in cattle mGSCs [110]. Another study showed that the expression of lncWNT3-IT could affect spermatogenesis in goats [36]. LncWNT3-IT is expressed in Sertoli cells and is related to the WNT3 protein which regulates proliferation and differentiation in testicular Sertoli cells [117,118].…”

Section: Lncrnas In Livestockmentioning

confidence: 99%

“…In goat testes, the proliferation of Sertoli cells was shown to be upregulated with increased expression levels of WNT3 producing overexpression of lncWNT3-IT. In a previous study, He et al [36] showed that lncWNT3-IT enhances WNT3 gene expression by clustering near to the WNT3 gene promoter and modulates the translation of WNT3 in a cis-acting manner, thereby regulating the growth cycle in goat Sertoli cells. Another lncRNA, lncNONO-AS, has been identified in goat testes.…”

Section: Lncrnas In Livestockmentioning

confidence: 99%

“…With the development of high throughput sequencing technology, many functional lncRNAs have been discovered, and many of these lncRNAs are thought to be involved in the formation of spermatozoa [33][34][35][36]. The expression profiles of lncRNAs in germ cells at different developmental stages were identified in mouse testes using gene chips and a large number of differentially expressed lncRNAs (DELs) were detected at different developmental stages [37].…”

Section: Introductionmentioning

confidence: 99%

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Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

Zhao

Heng

Sahlu

et al. 2021

IJMS

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Long noncoding RNAs (lncRNAs) are composed of nucleotides located in the nucleus and cytoplasm; these are transcribed by RNA polymerase II and are greater than 200 nt in length. LncRNAs fulfill important functions in a variety of biological processes, including genome imprinting, cell differentiation, apoptosis, stem cell pluripotency, X chromosome inactivation and nuclear transport. As high throughput sequencing technology develops, a substantial number of lncRNAs have been found to be related to a variety of biological processes, such as development of the testes, maintaining the self-renewal and differentiation of spermatogonial stem cells, and regulating spermatocyte meiosis. These indicate that lncRNAs can be used as biomarkers and potential therapeutic targets for male infertility. However, only a few comprehensive reviews have described the role of lncRNAs in male reproduction. In this paper, we summarize recent findings relating to the role of lncRNAs in spermatogenesis, their potential as biomarkers for male infertility and the relationship between reproductive arrest and transgenerational effects. Finally, we suggest specific targets for the treatment of male infertility from the perspective of lncRNAs.

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“…Recent studies have confirmed that lncRNAs are associated with brown fat formation ( Alvarez-Dominguez et al, 2015 ), skeletal muscle development ( Sun et al, 2016 ), and cashmere growth ( Wang et al, 2017 ). In 2020, He et al (2020) proposed that LncWNT3-IT can promote the proliferation of goat Sertoli cells by positively regulating the expression of the WNT3 gene. In addition, lncRNAs are also involved in the regulation of diseases.…”

Section: Introductionmentioning

confidence: 99%

LncRNA-mRNA modules involved in goat rumen development: Insights from genome-wide transcriptome profiling

Zhong

Zhao²,

Zhan³

et al. 2022

Front. Physiol.

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The rumen is an essential digestive and absorption organ of ruminants. During fetal life, lactation, and post-weaning period, goat rumen undergoes drastic morphological and metabolic-functional changes triggered by potential regulated genes and non-coding RNA molecules. As the essential regulatory factors, long non-coding RNAs (lncRNAs) have vital functions in various biological activities. However, their roles during rumen development are still poorly explored in ruminants. To explore the genome-wide expression profiles of lncRNAs and mRNAs in the goat rumens, we generated 5,007 lncRNAs and 19,738 mRNAs identified during the fetal and prepubertal stages by the high-throughput RNA sequencing. Notably, 365 lncRNAs and 2,877 mRNAs were considered to be differentially expressed. The weighted gene co-expression network analysis and functional analysis were performed to explore the regulatory roles of those differentially expressed molecules. The cis-and trans-target genes of differently expressed lncRNAs were enriched for pathways related to focal adhesion, cGMP-PKG signaling pathway, alpha-linolenic acid metabolism, arachidonic acid metabolism, and fat digestion and absorption. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes analyses showed that the differently expressed genes mainly participated in mitotic cytokinesis, desmosome, fatty acid degradation, cell adhesion molecules, and fatty acid metabolism. The prediction of lncRNA-mRNA interaction networks further revealed transcripts potentially involved in rumen development. The present study profiles a global overview of lncRNAs and mRNAs during rumen development. Our findings provide valuable resources for genetic regulation and molecular mechanisms of rumen development in ruminants.

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LncWNT3‐IT affects the proliferation of Sertoli cells by regulating the expression of the WNT3 gene in goat testis

Cited by 7 publications

References 32 publications

Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming

Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

LncRNA-mRNA modules involved in goat rumen development: Insights from genome-wide transcriptome profiling

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