LNK deficiency promotes acute aortic dissection and rupture

Critical role of IL-21 and T follicular helper cells in hypertension and vascular dysfunction

“…Slides were labeled with anti-CD3 (ab16669, Abcam, 1:250 dilution), anti-B220 (553086, BD Pharmingen, 1:20,000 dilution), or PNA (B1075, Vector, 1:250 dilution) to detect T cells, B cells, and GC B cells, respectively. For collagen staining, slides were stained with Masson's trichrome blue or Picrosirius red as previously described (19,36). Slides were scanned at ×20 using a Leica SCN400 Slide Scanner and visualized using Digital Image Hub software (Leica Biosystems).…”

Multimodality Imaging-Based Characterization of Regional Material Properties in a Murine Model of Aortic Dissection

“…Although aortic lesions that arise in the AngII-infused ApoE −/− mouse do not phenocopy human dissections in many regards -they occur in the abdominal, not thoracic, aorta near major branch sites, they occur at the interface between the media and adventitia rather than within the media, they do not result from a connective tissue disorder, and they do not appear to involve focal accumulations of glycosaminoglycans -they nevertheless represent an important model for study. Recent studies have used this model to gain insight into various molecular components of aortic dissection, including lymphocyte adaptor protein deficiency 36 , smooth muscle α-actin deficiency 37 , ciprofloxacin treatment 38 , and transforming growth factor-β-SMAD3 signaling 39,40 .…”

Co-localization of microstructural damage and excessive mechanical strain at aortic branches in angiotensin-II-infused mice

“…Ex vivo biaxial mechanical testing can also be used to explore biomechanical mechanisms of aortic dissection in mouse models. In particular, load-induced intramural delaminations have been observed in aortas of mice that have a propensity to dissect in vivo (Ferruzzi et al 2016;Laroumanie et al 2018). Here, for the first time, we use synchrotron imaging (PCXTM at 6.5 m isotropic resolution)…”

“…Numerous imaging modalities, including in vivo magnetic resonance imaging, high frequency ultrasound and ex vivo panoramic digital image correlation, have been used to estimate regional biomechanics in the AngII-infused murine abdominal aorta (Adelsperger et al 2018;Favreau et al 2012; Genovese et al 2012;Goergen et al 2011). Biaxial mechanical testing has also shed light on the solid mechanics of the mouse aortic wall prior to and during AngII infusion (Bersi et al 2017;Laroumanie et al 2018). Mouse-specific fluid dynamics simulations have been used to understand flow patterns in the true aortic lumen either prior to AngII infusion (Trachet et al 2011) or in the late stages of disease development when a false lumen is present (Ford et al 2011;Phillips et al 2017).…”