lntraovarian Secretion of Catecholamines, Oxytocin, Beta-Endorphin, and Gamma-Amino-Butyric-Acid in Freely Moving Rats: Development of a Push-Pull Tubing Method1

Wolf, Rainer; Meier-Fleitmann, A.; Düker, E.; Wuttke, W.

doi:10.1095/biolreprod35.3.599

Cited by 21 publications

(10 citation statements)

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“…These divergences might be due to the inability of PMSG to reproduce the ovarian physiological changes that occur during puberty . Additionally, using the ovary push-pull perifusate method, an increase in the NE content during the gonadotropin preovulatory peak is detected in rats with freedom of movement (Wolf et al, 1986), which indicates that the noradrenergic neurons involved in the control of the ovarian function are activated during the proestrus. Likewise, the release of [H 3 ] NE from the ovary, after its electric stimulation in vitro, is higher in animals in proestrus and estrus than during other phases of the estrus cycle (Ferruz et al, 1991).…”

Section: Catecholamines and Adrenergicmentioning

confidence: 95%

Role of the central and peripheral nervous system in the ovarian function

Aguado

2002

Microscopy Res & Technique

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This review attempts to give a comprehensive overview of ovarian innervation, considering the whole nervous system and its different levels that may modify the ovarian function. The connection between the ovary and the central nervous system through the autonomic pathways, including the peripheral ganglia, is highlighted. The evidence obtained over the last years highlights the role of the superior ovarian nerve (SON) in the ovarian phenomena. Besides, the effect on the ovary of conventional neurotransmitters and others such as indolamines and peptides, which have been found in this organ, are discussed. Various reproductive diseases have been studied almost exclusively from the endocrine point of view. It is evident that a better knowledge about the role of the neural factors involved in the ovarian physiology may facilitate the understanding of some of these. A review of the concepts and an update of some experimental designs is made that permits clarifying several aspects of the relationship between the neural system and the ovary. At present, there is no doubt that the innervation of the ovary is involved in several physiological aspects of this gland function. However, the relationship of some levels of the nervous system and the ovary offer a wide avenue for future research.

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Section: Catecholamines and Adrenergicmentioning

confidence: 95%

Role of the central and peripheral nervous system in the ovarian function

Aguado

2002

Microscopy Res & Technique

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“…Ovarian NE content has been found to be lower in large than in small antral follicles, but to increase markedly in preovulatory follicles (Veldhuis et al 1980). That these changes in ovarian NE may be related to an increased preovulatory release of NE from the nerve terminals is suggested by several observations, including an increase in NE content in porcine follicular fluid before ovulation (Bahr and Ben-Jonathan 1985), and an enhancement of NE levels in ovarian perfusates at the time of the preovulatory luteinizing hormone (LH) surge (Wolf et al, 1986). Lara and Belmar (1991) used the isolated cat ovary to study the biochemical organization of noradrenergic nerve terminals, and to deter- Fig.…”

Section: Content and Release Of Norepinephrine As An Index Ofmentioning

confidence: 99%

“…Wolf et al (1986) presented one study using a push-pull tubing method to study NE release from the ovary of freely moving rats. They found an increase in NE levels (measured with a radioenzymatic assay), in the ovary perfusates, during the preovulatory LH surge.…”

Section: Neurochemical Characterization Ofmentioning

confidence: 99%

“…Also the release of 3 H-NE recently taken up accepted as an index of neuronal activity presents several advantages as compared with other techniques. One difference from a push-pull technique, as used in the rat ovary by Wolf et al (1986), is that it does not need the whole animal, instead it uses only a small (10 -20 mg) piece of tissue that is incubated in vitro, making it extremely useful for studies in human biopsies (Lara et al, 2001) and for monitoring the activity of ovarian nerves during pathological states in a non-invasive, in vitro procedure and-as we did in the rat ovary through the estrous cycle (Fig. 4)-to obtain results similar to the ones obtained previously with the push-pull tubing method.…”

Section: Neurochemical Characterization Ofmentioning

confidence: 99%

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Changes in sympathetic nerve activity of the mammalian ovary during a normal estrous cycle and in polycystic ovary syndrome: Studies on norepinephrine release

Lara

Dorfman

Venegas

et al. 2002

Microscopy Res & Technique

119

113

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Although it has been known for many years that the ovary is innervated by catecholaminergic nerve fibers and much experimental evidence has strengthened the notion that catecholamines are physiologically involved in the control of ovarian function, scarce evidence has been presented as to the role of sympathetic activity in ovarian pathologies that affect reproductive function. The purpose of this article is to provide a succinct overview of the findings in this area and discuss them relative to the pathology of polycystic ovary syndrome, the most common ovarian pathology in women during their reproductive years.

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“…It is also clear that the rat ovary contains the necessary elements for noradrenergic function, including the enzymes involved in norepinephrine (NE) biosynthesis (4,5), a substantial content of NE (6), and the ability to release the catecholamine in vivo during the estrous cycle and during pathological states (7,8), and in vitro in response to depolarization of nerve terminals (9,10). Furthermore, ␤-noradrenergic receptors are expressed in both the thecal and granulosa cell compartments of the gland, and these receptors are positively coupled to steroidogenic responses (11,12).…”

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confidence: 99%

Functional Development of the Ovarian Noradrenergic Innervation

et al. 2007

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A substantial fraction of the noradrenergic innervation targeting the mammalian ovary is provided by neurons of the celiac ganglion. Although studies in the rat have shown that noradrenergic nerves reach the ovary near the time of birth, it is unknown how the functional capacity of this innervation unfolds during postnatal ovarian development. To address this issue, we assessed the ability of the developing ovary to incorporate and release (3)H-norepinephrine. Incorporation of (3)H-norepinephrine was low during the first 3 wk of postnatal life, but pharmacological inhibition of norepinephrine (NE) neuronal uptake with cocaine showed that an intact transport mechanism for NE into nerve terminals is already in place by the first week after birth. Consistent with this functional assessment, the mRNA encoding the NE transporter was also expressed in the celiac ganglion at this time. During neonatal-infantile development [postnatal (PN) d 5-20], the spontaneous, vesicle-independent outflow of recently taken up NE was high, but the NE output in response to K(+)-induced depolarization was low. After PN d 20, spontaneous outflow decreased and the response to K(+) increased markedly, reaching maximal values by the time of puberty. Tyramine-mediated displacement of NE stored in vesicles, which displace vesicular NE, showed that vesicle-dependent NE storage becomes functional by PN d 12 and that vesicular release increases during the juvenile-peripubertal phases of sexual development. These results indicate that vesicular release of NE from ovarian noradrenergic nerves begins to operate by the third week of postnatal life, becoming fully functional near the time of puberty.

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lntraovarian Secretion of Catecholamines, Oxytocin, Beta-Endorphin, and Gamma-Amino-Butyric-Acid in Freely Moving Rats: Development of a Push-Pull Tubing Method1

Cited by 21 publications

References 0 publications

Role of the central and peripheral nervous system in the ovarian function

Role of the central and peripheral nervous system in the ovarian function

Changes in sympathetic nerve activity of the mammalian ovary during a normal estrous cycle and in polycystic ovary syndrome: Studies on norepinephrine release

Functional Development of the Ovarian Noradrenergic Innervation

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