Load-dependent surface nanomechanical properties of poly-HEMA hydrogels in aqueous medium

Li, Gen; Dobryden, Illia; Salazar-Sandoval, Eric Johansson; Johansson, Mats; Claesson, Per M.

doi:10.1039/c9sm01113g

Cited by 15 publications

(13 citation statements)

References 47 publications

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“…These are possibly due to the different contributions in each regime, with combined elastic and viscous contributions in the approach maps, and a predominant elastic contribution in the retraction maps. This also indicates the importance of analyzing both approaching and retraction regimes in AFM force volume mapping for soft materials, the surface dynamics of which should be taken into account and a complicated tip–surface interaction occurs in the measurements. , Moreover, commonly applied contact mechanics models such as Hertz and/or the DMT are limited for studying soft gels due to the substantial viscous contribution from those soft gels. We thus also evaluated surface stiffness parameters, which do not require any contact mechanics model fitting and can be more suitable for the direct comparison of the nanomechanical surface property of Muc gels and tMuc gels using the same AFM probe.…”

Section: Materials and Methodsmentioning

confidence: 99%

Glyco-Modification of Mucin Hydrogels to Investigate Their Immune Activity

Yan

Hjorth

Winkeljann

et al. 2020

ACS Appl. Mater. Interfaces

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Mucins are multifunctional glycosylated proteins that are increasingly investigated as building blocks of novel biomaterials. An attractive feature is their ability to modulate the immune response, in part by engaging with sialic acid binding receptors on immune cells. Once assembled into hydrogels, bovine submaxillary mucins (Muc gels) were shown to modulate the recruitment and activation of immune cells and avoid fibrous encapsulation in vivo. However, nothing is known about the early immune response to Muc gels. This study characterizes the response of macrophages, important orchestrators of the material-mediated immune response, over the first 7 days in contact with Muc gels. The role of mucin-bound sialic acid sugar residues was investigated by first enzymatically cleaving the sugar and then assembling the mucin variants into covalently cross-linked hydrogels with rheological and surface nanomechanical properties similar to nonmodified Muc gels. Results with THP-1 and human primary peripheral blood monocytes derived macrophages showed that Muc gels transiently activate the expression of both pro-inflammatory and anti-inflammatory cytokines and cell surface markers, for most makers with a maximum on the first day and loss of the effect after 7 days. The activation was sialic acid-dependent for a majority of the markers followed. The pattern of gene expression, protein expression, and functional measurements did not strictly correspond to M1 or M2 macrophage phenotypes. This study highlights the complex early events in macrophage activation in contact with mucin materials and the importance of sialic acid residues in such a response. The enzymatic glyco-modulation of Muc gels appears as a useful tool to help understand the biological functions of specific glycans on mucins which can further inform on their use in various biomedical applications.

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Section: Materials and Methodsmentioning

confidence: 99%

Glyco-Modification of Mucin Hydrogels to Investigate Their Immune Activity

Yan

Hjorth

Winkeljann

et al. 2020

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…These are possibly due to the different contributions in each regime, with a combined elastic and viscous contributions in the approach maps, and a predominant elastic contribution in the retraction maps. This also indicates the importance of analysing both approaching and retraction regimes in AFM force volume mapping for soft materials, the surface dynamics of which should be taken into account and a complicated tip-surface interaction occurs in the measurements 33,58 . Moreover, commonly applied contact mechanics models such as Hertz and/or the Derjaguin-Muller-Toporov (DMT) are limited for studying soft gels due to the substantial viscous contribution from those soft gels.…”

Section: Rheological Characterization Of Muc Gelsmentioning

confidence: 97%

Mucin hydrogel glyco-modulation to investigate immune activity of mucin glycans

Yan

Hjorth

Winkeljann

et al. 2019

Preprint

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Mucins are multifunctional glycosylated proteins that are increasingly investigated as building blocks of novel biomaterials. Once assembled into hydrogels (Muc gels), mucins were shown to modulate the recruitment and activation of immune cells and avoid fibrous encapsulation in vivo.However, nothing is known about the early immune response to Muc gels. This study characterizes the response of macrophages, important orchestrators of the material-mediated immune response, over the first 7 days in contact with Muc gels. The role of mucin-bound sialic acid sugar residues was investigated by first enzymatically cleaving the sugar, then assembling the mucin variants into covalently crosslinked hydrogels with rheological and surface nanomechanical properties similar to non-modified Muc gels. Results with THP1 and human primary peripheral blood monocytesderived macrophages were strikingly consistent and showed that Muc gels transiently activate the expression of both pro-inflammatory and anti-inflammatory cytokines and cell surface markers, with a maximum on the first day and loss of the effect after 7 days. The activation was sialic aciddependent for a majority of the markers followed. The pattern of gene expression, protein expression, and functional measurements did not strictly correspond to M1 or M2 macrophage phenotypes. This study highlights the complex early events in macrophage activation in contact with mucin materials and the importance of sialic acid residues in such a response. The enzymatic glyco-modulation of Muc gels appears as a useful tool to help understand the biological functions of specific glycans on mucins which can further inform on their use in various biomedical applications.

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“…At stimuliresponsive hydrogels, which demonstrate great potential in various pharmaceutical and biomedical applications, have been one of the more recent developments in carrier material science [13]. Hydrogels have various uses in biomedical applications [14]. At the same time, they are well suited for regenerative medicine and controlled drug delivery [15].…”

Section: Introductionmentioning

confidence: 99%

Nifedipine, Niclosamide and Furosemid Release of A Biocompatible and Protective Hydrogel for Cancer Therapy

Yılmaz¹

2021

Preprint

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In this work, biocompatible and protective pure HEMA hydrogel structures and drug loaded HEMA hydrogel have been successfully synthesized. The hydrogel was prepared using HEMA, MBA, APS and TEMED. The structural and morphological characterization of pure hydrogel and drug-loaded hydrogels were characterized using Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM), respectively. The pH affinity, morphology, structure, drug release, swelling and cytotoxic effect of the resulting materials were studied in detail. Drug releases of drug-loaded hydrogel structures were measured at certain time intervals and recorded as cumulatively (%). In addition, cytotoxicity tests were performed by Alamar blue method on MCF-7, MIA PaCa-2 and HEK 293 cell lines of released drug molecules. This HEMA hydrogel was suggested to be materials of biological attention and of large pharmacological potential as support material in drug release of nifedipine, furosemide, niclosamide and similar drug molecules.

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Load-dependent surface nanomechanical properties of poly-HEMA hydrogels in aqueous medium

Abstract: The combined action of load and shear results in the formation of a temporary sub-micrometer hill in front of the tip. As the tip pushes against such hills, a pronounced stick-slip effect is observed for the hydrogel with low cross-linking density.

Cited by 15 publications

References 47 publications

Glyco-Modification of Mucin Hydrogels to Investigate Their Immune Activity

Glyco-Modification of Mucin Hydrogels to Investigate Their Immune Activity

Mucin hydrogel glyco-modulation to investigate immune activity of mucin glycans

Nifedipine, Niclosamide and Furosemid Release of A Biocompatible and Protective Hydrogel for Cancer Therapy

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