Loading and Releasing Behavior of Selenium and Doxorubicin Hydrochloride in Hydroxyapatite with Different Morphologies

Gao, Jing; Huang‬‬‬‬, Jinhui Jeanne; Shi, Rui; Wei, Jiawei; Lei, Xiaoyu; Dou, Yichen; Li, Yubao; Zuo, Yi; Li, Jidong

doi:10.1021/acsomega.1c00092

Cited by 13 publications

(6 citation statements)

References 57 publications

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“…Since, in these regions, the polymers are only physically bound, they can leach out to the surrounding solution [ 72 ], thereby imparting antibacterial activity to it [ 73 ]. This phenomenon is expected to be more prominent in the case of the PIIID-HA discs owing to the significant thickness and porous nature of the HA discs [ 73 , 74 ]. Furthermore, the PIIID-generated layer on the glass surface was observed to be mildly unstable (data not reported).…”

Section: Resultsmentioning

confidence: 99%

Electrostatic and Covalent Binding of an Antibacterial Polymer to Hydroxyapatite for Protection against Escherichia coli Colonization

et al. 2023

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Orthopedic-device-related infections are notorious for causing physical and psychological trauma to patients suffering from them. Traditional methods of treating these infections have relied heavily on antibiotics and are becoming ineffectual due to the rise of antibiotic-resistant bacteria. Mimics of antimicrobial peptides have emerged as exciting alternatives due to their favorable antibacterial properties and lack of propensity for generating resistant bacteria. In this study, the efficacy of an antibacterial polymer as a coating material for hydroxyapatite and glass surfaces, two materials with wide ranging application in orthopedics and the biomedical sciences, is demonstrated. Both physical and covalent modes of attachment of the polymer to these materials were explored. Polymer attachment to the material surfaces was confirmed via X-ray photoelectron spectroscopy and contact angle measurements. The modified surfaces exhibited significant antibacterial activity against the Gram-negative bacterium E. coli, and the activity was retained for a prolonged period on the surfaces of the covalently modified materials.

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Section: Resultsmentioning

confidence: 99%

Electrostatic and Covalent Binding of an Antibacterial Polymer to Hydroxyapatite for Protection against Escherichia coli Colonization

et al. 2023

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“…Through immunohistochemistry, histology and scanning electronic microscope analysis, the factors involved in bone regeneration processes can be characterized in a broader and easier way [25]. A study that analyzed the use of Masson's trichrome, metaloproteinase (mmp-9), periodic acid Schiff (PAS), tumor necrosis factor (TNF-alpha) and associated ultrastructural results in non-critical defects in rat calvaria compared the use of clots, xenogeneic hydroxyapatite and synthetic nano-hydroxyapatite, reaching the conclusion that nano-hydroxyapatite presented a more favorable inflammatory response, formation of a more relevant bone matrix and it was possible to identify some structures involved in the bone repair process through electron microscopy of sweep [26].…”

Section: Discussionmentioning

confidence: 99%

Osteopontin and Vascular Endothelial Growth Factor-Immunoreactivity in Critical Bone Defects Matrix Production: A Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogeneic Hydroxyapatite Comparison

et al. 2021

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The development of new bone substitutes has become an area of great interest in materials science. In fact, hydroxyapatite is the most commonly used biomaterial in defects that require bone reconstruction, and that is certainly why the discovery of new products with its formulation has been increasing continuously. The aim of this study was to analyze the biological behavior of a xenogeneic hydroxyapatite widely disclosed in the literature and a synthetic nano-hydroxyapatite/Beta tricalcium phosphate in critical defects in the calvaria of Wistar rats. For this, the groups were divided as follows: 24 adult male Wistar rats were used, weighing between 300 and 350 g, in three groups with eight animals each. In the CTRL group (control), only the clot was kept, without material insertion; in the Bioss group (bovine hydroxyapatite), Bio Oss®—Gleistlich® was introduced; and in the Blue Bone group (REG), the defect was filled in with synthetic nano-hydroxyapatite associated with betatriphosphate of calcium, Blue Bone®—Regener®. According to the results in Goldner’s Trichromics, we can observe a higher percentage of newly formed bone matrix in the REG group than in the CTRL and Bioss groups; in the VEGF, we had a more adequate cell modulation for blood vessel formation in the Blue Bone group (REG) compared to the Bioss and CTRL groups, while in osteopontin, a higher percentage of bone formation was observed in the Blue Bone group (REG) and Bioss group when compared to the CTRL group. We conclude that bone formation, mitosis-inducing cell modulation and main osteoblast activity were higher in the Blue Bone group (REG) than in the CTRL and Bioss groups.

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“…The particle aggregation over the surface caused by the production of enormous surface-interface atoms was due to decreased particle size, and it also induced the stress-strain factors [47]. The introduction of selenium oxide was responsible for smaller particle size (S > S3 > S5 > S10) and a large surface area that formed aggregation of particles due to electrostatic attraction and enhanced particle interaction [21]. Further, under the influence of increased selenium oxide content, the densely packed structure is evident over the material's surface, producing fine-sized materials with improved particle interaction efficiency.…”

Section: Fesem-eds Analysismentioning

confidence: 99%

“…The small particle size enhanced surface effects by increasing the surface area to volume ratio, which induced a high degree of reactivity with physiological fluid. These materials are able to readily pass through the blood-bone barrier without losing their properties due to their fine size [21,22]. Parallel to this, the functionally designed surface of the material enhances its osteoinductivity, thereby improving its biomechanical properties [23].…”

Section: Introductionmentioning

confidence: 99%

Calcium silicate biocomposites: effects of selenium oxide on the physico-mechanical features and their in-vitro biological assessments

et al. 2023

Biomed. Mater.

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Bone tissue regenerative material serves as a prospective recovery candidate with self-adaptable biological properties of bio-activation, degradability, compatibility, and antimicrobial efficacy instead of metallic implants. Such materials are highly expensive due to chemical reagents and complex synthesis procedures, making them unaffordable for patients with financial constraints. This research produced an efficient bone tissue regenerative material using inexpensive naturally occurring source materials, including silica sand and limestone. The extracted SiO2 and CaO particles (75:25 wt%) were subjected to hydrothermal synthesis (water treatment instead of chemical solvents) to produce the CaSiO3 biomaterial (code: S). Selenium oxide was doped with calcium silicate at 3, 5, and 10 wt.% to enhance its properties, yielding biocomposite materials (i.e., S3, S5, and S10). The physico-mechanical properties of these materials were investigated with XRD, FTIR, FESEM-EDS, and micro-UTM. The results revealed that the synthesized biocomposites have a crystalline wollastonite phase with a porously fused rough surface. From structural parametric calculations, we found that the biocomposites have reduced particle size and enhanced surface area due to the influence of selenium oxide. The biocomposite S10, having high SeO2 content, attained the maximum compressive strength of 75.2 MPa. In-vitro studies of bioactivity, biodegradability, biocompatibility, and antibacterial activity were performed. At 7 and 14 days of bioactivity, the synthesized biocomposites are capable of dissolving their ions into SBF solution to precipitate HAp and a required Ca/P ratio of 1.69 was achieved by S3. A comparative analysis has been performed on the degradation activity in Tris-HCl and the consequent pH changes during SBF treatment. The bio-analysis revealed that the biocomposite S3 shows enhanced bioactivity through a controlled degradation rate and secured cell viability of 88% at a concentration of 100 μg/ml. It also offers significant bacterial inhibition potency against E.coli and S.aureus bacteria.

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Loading and Releasing Behavior of Selenium and Doxorubicin Hydrochloride in Hydroxyapatite with Different Morphologies

Cited by 13 publications

References 57 publications

Electrostatic and Covalent Binding of an Antibacterial Polymer to Hydroxyapatite for Protection against Escherichia coli Colonization

Electrostatic and Covalent Binding of an Antibacterial Polymer to Hydroxyapatite for Protection against Escherichia coli Colonization

Osteopontin and Vascular Endothelial Growth Factor-Immunoreactivity in Critical Bone Defects Matrix Production: A Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogeneic Hydroxyapatite Comparison

Calcium silicate biocomposites: effects of selenium oxide on the physico-mechanical features and their in-vitro biological assessments

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