Loading induces site-specific increases in mineral content assessed by microcomputed tomography of the mouse tibia

Fritton, J. Christopher; Myers, Elizabeth; Wright, Timothy M.; Meulen, Marjolein C. H. van der

doi:10.1016/j.bone.2005.02.013

Cited by 212 publications

(278 citation statements)

References 39 publications

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“…Furthermore when applying the same loading regimes to 12 and 20 week old mice trabecular bone mass was shown to decline. In another study Fritton et al (22) reported similar 15 % increases in tibial trabecular BV/TV for two groups of female B6 mice (10 weeks of age). These mice were loaded for 2 weeks and 6 weeks using the same mechanical signal.…”

Section: Trabecular Dose Dependency Was Not Inferred By Histomorphomementioning

confidence: 79%

“…Two other studies using a murine tibia loading model (21,22) have shown cancellous bone to respond to mechanical stimuli, however the reported effects were not as pronounced. Souza et al (21) was unable to show a significant anabolic effect between loading groups when loading 8 week old B6 mice, however a 37% increase in trabecular BV/TV was achieved when combining the data from all loading groups.…”

Section: Trabecular Dose Dependency Was Not Inferred By Histomorphomementioning

confidence: 84%

“…De Souza et al (21) mechanically stimulated mice tibia using dynamic loads with magnitudes between 5 and 13 N and demonstrated a significant increase in trabecular bone volume density when pooling all the loaded groups together. Using a similar approach Fritton et al (22) showed trabecular bone volume density and trabecular thickness to increase significantly by 15 % and 12 % when applying a single 6 week long loading regimen. Here we present an alternative in vivo model for the study of both cortical and trabecular bone adaptation.…”

Section: Introductionmentioning

confidence: 99%

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Mechanical loading of mouse caudal vertebrae increases trabecular and cortical bone mass-dependence on dose and genotype

Webster

Wasserman

Ehrbar

et al. 2010

Biomech Model Mechanobiol

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Most in vivo studies addressing the skeletal responses of mice to mechanical loading have targeted cortical bone. To investigate trabecular bone responses also we have developed a caudal vertebral axial compression device (CVAD) that transmits mechanical loads to compress the fifth caudal vertebra via stainless steel pins inserted into the forth and sixth caudal vertebral bodies. Here, we used the CVAD in C57BL/6 (B6) and C3H/Hej (C3H) female mice (15 weeks of age) to investigate whether the effect of regular bouts of mechanical stimulation on bone modeling and bone mass was dependent on dose and genotype. A combined micro-computed tomographic and dynamic histomorphometric analysis was carried out at the end of a 4-week loading regimen (3,000 cycles, 10 Hz, 3 x week) for load amplitudes of 0N, 2N, 4N and 8N. Significant increases in trabecular bone mass of 9 and 21% for loads of 4N and 8N, respectively, were observed in B6 mice. A significant increase of 10% in trabecular bone mass occurred for a load of 8N in the C3H strain. For other loads, no significant increases were detected. Both mouse strains exhibited substantial increases in trabecular bone formation rates for all loads, B6: 111% (2N), 86% (4N), 164% (8N), C3H: 41% (2N), 38% (4N), 141% (8N). Significant decreases in osteoclast number of 146 and 93% for a load of 8N were detected in B6 and C3H mice, respectively. These findings demonstrate that the effect of loading on the structural and functional parameters of bone is dose and genotype dependent. The caudal vertebral loading model established here is proposed for further studies addressing the molecular processes involved in the skeletal responses to mechanical stimuli. Here we used the CVAD in C57BL/6 and C3H/Hej mice to investigate whether the effect of regular bouts of mechanical stimulation on bone remodeling and bone mass were dependent on dose and genotype. A combined micro-computed tomographic and dynamic histomorphometric analysis carried out at the end of a 4-week loading regimen for load amplitudes of 0N, 2N, 4N and 8N revealed that an amplitude of 8N stimulated significant increases in trabecular and cortical bone mass mainly in the C57BL/6 strain. Both biological strains exhibited substantial increases in bone formation rates and decreases in osteoblast number. These findings demonstrate that the effect of loading on the structural and functional parameters of bone is dose-and genotype dependent. The caudal vertebral loading model established here is proposed for further studies addressing the molecular processes involved in the skeletal responses to mechanical stimuli.

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Section: Trabecular Dose Dependency Was Not Inferred By Histomorphomementioning

confidence: 79%

Section: Trabecular Dose Dependency Was Not Inferred By Histomorphomementioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanical loading of mouse caudal vertebrae increases trabecular and cortical bone mass-dependence on dose and genotype

Webster

Wasserman

Ehrbar

et al. 2010

Biomech Model Mechanobiol

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show abstract

“…The curvature of the mouse tibia induces bending moments that produce compressive and tensile strains at the fracture site. Strains induced at the middiaphysis with loading have been characterized for intact tibia of mice using this loading device [9,17], but the strains that occur with loading across a stabilized osteotomy in this model are unknown.…”

Section: Discussionmentioning

confidence: 99%

“…In three of the groups, cyclic compression was applied to the ends of the tibia with a noninvasive external loading device ( Fig. 1) [17]. Daily loading was begun after a 4-day fracture consolidation period to allow for stabilization of the hematoma and early angiogenesis [19,34] and continued for 5 days per week for 2 weeks.…”

Section: Methodsmentioning

confidence: 99%

Pause Insertions During Cyclic In Vivo Loading Affect Bone Healing

Gardner

Ricciardi

Wright

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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Fracture repair is influenced by the mechanical environment, particularly when cyclic loads are applied across the fracture site. However, the specific mechanical loading parameters that accelerate fracture healing are unknown. Intact bone adaptation studies show enhanced bone formation with pauses inserted between loading cycles. We hypothesized pause-inserted noninvasive external loading to mouse tibial fractures would lead to accelerated healing. Eighty mice underwent tibial osteotomies with intramedullary stabilization and were divided into four loading protocol groups: (1) repetitive loading (100 cycles, 1 Hz); (2) pause/time-equivalent (10 cycles, 0

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Allen's Rule Revisited: Temperature Influences Bone Elongation During a Critical Period of Postnatal Development

Serrat

2013

The Anatomical Record

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Limbs of animals raised at warm ambient temperature are significantly and permanently longer than those of siblings housed in the cold. These highly reproducible lab results closely parallel the ecogeographical tenet described by Allen's extremity size rule, which states that appendage length correlates with temperature and latitude. It is unclear what mechanisms underlie these differences and in what pattern they emerge, since the morphology is traditionally thought to reflect naturally selected genomic adaptations for thermoregulation. This study tests the a posteriori hypothesis that adult extremity length is subject to substantial modification by temperature during a brief but critical period of early postnatal development. Weanling mice (N 5 28) were divided into three groups and housed at 7 C, 21 C, or 27 C for eight weeks. Tail lengths and body mass were measured weekly. Mass did not differ at any age. Analysis of tail elongation curves revealed two distinct phases: an initial period of rapid temperature-sensitive growth in which elongation rate was directly impacted by temperature; and a second phase of continued growth in which rates were identical among groups. Comparable growth reactions occur in response to other environmental variables such as exercise, suggesting that the skeleton is most responsive to external stimuli during a window of heightened sensitivity when growth occurs most rapidly. Knowledge of the timing and degree to which growth plasticity permits mammals to immediately adjust to novel temperature conditions will be important for analyzing skeletal variation in fluctuating climates, particularly for assessing factors that may accelerate skeletal evolution at temperature extremes. Anat Rec, 296:1534Rec, 296: -1545

show abstract

Loading induces site-specific increases in mineral content assessed by microcomputed tomography of the mouse tibia

Cited by 212 publications

References 39 publications

Mechanical loading of mouse caudal vertebrae increases trabecular and cortical bone mass-dependence on dose and genotype

Mechanical loading of mouse caudal vertebrae increases trabecular and cortical bone mass-dependence on dose and genotype

Pause Insertions During Cyclic In Vivo Loading Affect Bone Healing

Allen's Rule Revisited: Temperature Influences Bone Elongation During a Critical Period of Postnatal Development

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