Loading of halloysite nanotubes with BSA,α-Lac andβ-Lg: a Fourier transform infrared spectroscopic and thermogravimetric study

Abstract

Halloysite nanotubes (HNTs) are considered as ideal materials for biotechnological and medical applications. An important feature of halloysite is that it has a different surface chemistry on the inner and outer sides of the tubes. This property means that negatively-charged molecules can be selectively loaded inside the halloysite nanoscale its lumen. Loaded HNTs can be used for the controlled or sustained release of proteins, drugs, bioactive molecules and other agents. We studied the interaction between HNTs and bovine serum albumin, α lactalbumin and β -lactoglobulin loaded into HTNs using Fourier transform infrared spectroscopy and thermogravimetry. These techniques enabled us to study the protein conformation and thermal stability, respectively, and to estimate the amount of protein loaded into the HNTs. TEM images confirmed the loading of proteins into HTNs.

Production and characterization of supercritical CO2 dried chitosan nanoparticles as novel carrier device

“…The DTG of the Cs shown a maximum at 295°C which corresponds to the beginning of the 296 degradation of the polymer. For the lg it has been reported three main peaks around 300°C, 4λ0°C 297 and 583°C (Duce et al, 2017). In our study the DTG of lg has two main peaks at 300 and 550°C.…”

“…been described previously and the losses at 4λ0°C ( lg) can be related to the decomposition of 284 aggregated portions of proteins, while the sharp mass loss above 550°C is related to the carbonizing 285 and ashing of the hard residues of the proteins (Duce et al, 2017). 286…”

Halloysite nanotubes as support for metal-based catalysts

“…1a). There are several elds of application for this smart nanomaterial, including drug transport and delivery, 10,[13][14][15][16][17][18][19] environmental remediation, 20-26 as a ller in polymeric matrices, 27-30 ame retardant 31 and support for catalysts 13,23 (Fig. 1b).…”

Physico–Chemical Interaction between Clay Minerals and Albumin Protein according to the Type of Clay

“…Compared with the primary and secondary structure, the tertiary structure, which determines the function of the protein by controlling the three-dimensional structure, is formed by relatively weak forces, such as van der Waals interactions, occasional disulfide bonds, etc., and thus the tertiary structure can be influenced by external chemical and physical stimuli. There have been reports that complicated denaturation phenomena of proteins upon reaction with nanoparticles can be quantitatively evaluated by the protein fluorescence quenching assay [28][29][30], although we cannot clarify the origin of denaturation with this method. In order to find biomedical applications of clays, one must evaluate how the chemical and physical properties of clays affect both the secondary structure and general 3-dimensional structure of proteins.…”

“…It is possible for clays to accommodate proteins in their intra-or inter-particular space. Tubular clays, like halloysite nanotubes (HNT), can accommodate proteins inside the tube lumen [26][27][28][29]. When proteins are incorporated into clays, there are concerns that the conformation of the protein may change, as the three-dimensional shape is strongly related to the function of the protein.…”