Loading red blood cells with trehalose: a step towards biostabilization

Satpathy, Gyana R.; Török, Zsolt; Bali, Rachna; Dwyre, Denis M.; Little, Erika; Walker, Naomi J.; Tablin, Fern; Crowe, John H.; Tsvetkova, Nelly M.

doi:10.1016/j.cryobiol.2004.06.001

Cited by 121 publications

(110 citation statements)

References 40 publications

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“…This is due to a combination of osmotic imbalance and modulation of the phospholipid phases, giving an intracellular concentration up to 150 mM. Higher values can be obtained by using mixtures with trehalose and polysaccharides (HES) [75,76].…”

Section: T I O Nmentioning

confidence: 99%

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Cordone¹,

Cupane²,

Emanuele

et al. 2015

COC

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Immobilization of proteins and other biomolecules in saccharide matrices leads to a series of peculiar properties that are relevant from the point of view of both biochemistry and biophysics, and have important implications on related fields such as food industry, pharmaceutics, and medicine. In the last years, the properties of biomolecules embedded into glassy matrices and/or highly concentrated solutions of saccharides have been thoroughly investigated, at the molecular level, through in vivo, in vitro, and in silico studies. These systems show an outstanding ability to protect biostructures against stress conditions; various mechanisms appear to be at the basis of such bioprotection, that in the case of some sugars (in particular trehalose) is peculiarly effective.Here we review recent results obtained in our and other laboratories on ternary protein-sugar-water systems that have been typically studied in wide ranges of water content and temperature. Data from a large set of complementary experimental techniques provide a consistent description of structural, dynamical and functional properties of these systems, from atomistic to thermodynamic level.In the emerging picture, the stabilizing effect induced on the encapsulated systems might be attributed to a strong biomolecule-matrix coupling, mediated by extended hydrogen-bond networks, whose specific properties are determined by the saccharide composition and structure, and depend on water content.

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Section: T I O Nmentioning

confidence: 99%

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Cordone¹,

Cupane²,

Emanuele

et al. 2015

COC

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“…RBC membrane damage, hemoglobin leakage, high hemolysis ratio, and reduction of ATPase, G-6-PD and SOD activities are common problems that result from RBC lyophilization [4,14]. The damages result from the water loss that occurs during lyophilization [15]. Fortunately, these problems can be resolved using lyophilization protectants.…”

Section: Discussionmentioning

confidence: 99%

“…Fortunately, these problems can be resolved using lyophilization protectants. Trehalose and saccharose are two commonly used lyophilization protectants [15,16], but the synergistic effects of trehalose and saccharose pretreatment on the maintenance of lyophilized RBC quality are unknown. We studied the synergistic effects of trehalose and saccharose pretreatment on the maintenance of lyophilized RBC quality by determining RBC counts recovery rate, total hemoglobin concentration recovery rate, MCH, MCV, comprehensive deformation index, hemolysis ratio, PS expression, and SOD, ATPase, and G-6-PD activities.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Trehalose and Saccharose Pretreatment on Maintenance of Lyophilized Human Red Blood Cell Quality

Li¹,

Hu²,

Zhong³

et al. 2016

Trop. J. Pharm Res

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“…Trehalose, a small disaccharide molecule of glucose, is considered safe to consume [11] and possesses an exceptional ability to stabilize the biomembrane and preserve cells and tissues during the freeze-thaw procedures [12]. Subsequently, a number of studies have shown that trehalose can be used as an excellent alternative cryoprotectant in the cryopreservation of red blood and germ cells [13][14][15]. Another advantage of trehalose, as previously demonstrated, is its ability to inhibit the apoptosis level [16,17].…”

Section: Introductionmentioning

confidence: 99%

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Lan

Zhao

et al. 2015

J Assist Reprod Genet

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Purpose The study aims to assess the protective effects of dimethyl sulfoxide (DMSO)-free solution based on trehalose on the cryopreservation of a whole sheep ovary and evaluate its use as an efficient cryoprotectant. Method Twenty-one ovaries collected from 6-to 8-month-old non-pregnant female sheep were randomly distributed into three groups, namely, a fresh group, a DMSO-free group, and a DMSO group. The morphology, cell apoptosis (by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyltransferasemediated dUTP nick-end labeling (TUNEL) assay), and mRNA transcript of Bcl-2-associated X protein (BAX) and cold inducible RNA-binding protein (CIRP) (by real-time PCR) of the thawed sheep ovaries and fresh controls were tested to establish a criterion for appraising the results of the cryopreservation.Results (i) The histological assessment indicated that the structure of the DMSO-free ovaries remained largely intact and comparable to those of the fresh control groups; whereas, significant damage was observed in the ovaries of the DMSO group (P<0.05). (ii) The TUNEL assay and mRNA transcript of the BAX assessment showed that the apoptosis parameter in the fresh group was the lowest among all the groups (P<0.05), and the parameter in the DMSO-free group was significantly lower than that in the DMSO group (P<0.05). (iii) The level of the CIRP transcripts increased the most in the DMSO-free group followed by the DMSO group and the fresh control group (P<0.05). Conclusions These results indicate that a DMSO-free cryoprotectant solution, especially a trehalose cryoprotectant, is an efficient cryoprotectant and has a beneficial effect on the cryopreservation of whole sheep ovaries.Capsule These results indicate that a DMSO-free and trehalose-containing cryoprotectant solution has a beneficial effect on the cryopreservation of intact sheep ovaries.Tianqi Du and Lan Chao contributed equally to this work.

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Loading red blood cells with trehalose: a step towards biostabilization

Cited by 121 publications

References 40 publications

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Synergistic Effect of Trehalose and Saccharose Pretreatment on Maintenance of Lyophilized Human Red Blood Cell Quality

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

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