Loblolly Pine Tissue Culture: Laboratory, Greenhouse, and Field Studies

Amerson, Henry V.; Frampton, L. J.; McKeand, Steven E.; Mott, R. L.; Weir, Robert J.

doi:10.1007/978-1-4899-0378-5_19

Cited by 24 publications

(12 citation statements)

References 7 publications

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“…A short, high concentration auxin pulse treatment is often more effective at inducing roots on shoots grown in vitro than a prolonged, low concentration treatment (Amerson & Mott, 1982;Amerson et al, 1985;von Arnold & Eriksson, 1986). Prolonged incubation on a medium rich in auxin can cause excessive callusing, delay root initiation or inhibit the elongation of root initials (Karnosky & Diner, 1984;Gronroos & von Arnold, 1987).…”

Section: Discussionmentioning

confidence: 99%

Adventitious root formation in hypocotyl cuttings of Picea sitchensis (Bong.) Carr. — the influence of plant growth regulators

1992

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SUMMARYA simple method for the study of adventitious root initiation on Sitka spruce [Picea sitchensis (Bong.) Carr.] hypocotyl cuttings is described and was used to examine the effects of plant growth regulators. Twenty-one-dayold seedlings, grown at a low light intensity (15-20 fimol m~^ s~^) to stimulate hypocotyl elongation, provided easy to handle tissues which were responsive to root inducing treatments. Spontaneous rooting only occurred at a low frequency on untreated cuttings and reliable rooting depended upon auxin treatment. When continuous auxin treatment was used, indole-3-butyric acid (IBA) was more effective than either indole-3-acetic acid (IAA) or 1-naphthaleneacetic acid (NAA), and was optimal between lO""" and 10~* M. Pulse treatments with 10~^ M IBA for as little as 10 min were sufficient to stimulate rooting. All other growth regulators examined were inhibitory to IBA induced rooting. 6-benzylaminopurine (BA) was the most active being inhibitory down to a concentration of 3 X 10"' M when it was applied in a mixture with IBA. Pre-treatment of hypocotyl cuttings with BA prior to auxin treatment demonstrated strong cytokinin 'carry-over' inhibitory effects on rooting. Gibberellic acid Ag (GAg), abscisie acid (ABA) and 2-chloroethyl-phosphoric acid (ethrel) were less potent inhibitors of rooting.

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Section: Discussionmentioning

confidence: 99%

Adventitious root formation in hypocotyl cuttings of Picea sitchensis (Bong.) Carr. — the influence of plant growth regulators

1992

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“…Those media without BAP did not support shoot differentiation and growth. The low ammonium/nitrate (3:10 raM) levels of GD1 and the replacement of ammonium by glutamine in BL were also adequate to support organogenesis in a loblolly pine (P. taeda L.) micropropagation system (Amerson et al 1985).…”

Section: Discussionmentioning

confidence: 99%

“…Effective rooting of adventitious shoots derived in vitro has limited the effective use of micropropagated conifer plantlets (Coleman and Thorpe 1977;Amerson et al 1985;Hanover and Keathley 1988). Root formation in vitro is the first step towards plantlet establishment ex vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Root formation in vitro is the first step towards plantlet establishment ex vitro. Among North American pines, loblolly pine has an established system for routine micropropagation (Amerson et al 1985). This is one of the first reports of rooted plantlet production of slash pine in vitro (Lesney et al 1988).…”

Section: Discussionmentioning

confidence: 99%

“…Three media formulas were tested: (1) modified Brown and Lawrence (BL) (Amerson et al 1985); (2) modified Gresshoff and Doy 1 (GD1) (Mehra-Palta et al 1978;Greshoff and Doy 1972); and (3) Risser and White's (RW) major salts (Risser and White 1964). All media included vitamins and Fe as Fe-EDTA (Greshoff and Doy 1972).…”

Section: Culture Mediamentioning

confidence: 99%

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Cultural factors influencing adventitious shoot and plantlet formation from slash pine cotyledons

Bronson

Dixon

1991

New Forest

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Application. Clonal propagation of woody plants, including conifers, is useful for tree improvement and forestation. However, complete regeneration of plantlets from propagules has been difficult to achieve with some Pinus species. An in vitro procedure for micropropagation of slash pine (Pinus elliotti) plantlets from excised cotyledons is presented.Abstract. Cultural factors affecting in vitro shoot and subsequent plantlet formation of slash pine (Pinus elliotti Engelm.) cotyledons were investigated. Basal media composition, N 6-benzylaminopurine (BAP) concentration and exposure time significantly influenced bud induction in cotyledons cultured under a continuous photoperiod of 35--40 #mol m -2 s -1 at 24 + 1 °C. The largest number of adventitious shoots was obtained after 28 days exposure to 66 /zM BAP-supplemented modified Gresshoff and Doy 1 (GD1) medium. Relatively high frequencies of large shoots were obtained after a 14-day exposure to 22/zM BAP-supplemented Brown and Lawrence (BL) or 66 ~M BAP-supplemented GD1. Adventitious shoots derived from 21-or 28-day exposures to BAP developed more slowly and were smaller in size than those derived from a 14-day exposure to the cytokinin. Shoot differentiation and subsequent growth were also influenced by basal media, media concentration, and presence of activated charcoal in the medium. The percentage of cotyledons forming shoots was highest on half-strength GD1 medium containing activated charcoal. Rooting was achieved in vitro under a continuous photoperiod of 60--70 /~mol m -2 s -1. Roots were formed when excised shoots were planted on GD 1/2 medium supplemented with 2.68 /zM 1-1 a-napthaleneacetic acid (NAA) with or without BAP for 14 days. The proposed technique of slash pine propagation using cotyledon explants can produce up to 100 seedlings per embryo.

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