LOC102724163 promotes breast cancer cell proliferation and invasion by stimulating MUC19 expression

Abstract

Breast cancer (BC) is a malignant disease and the most commonly diagnosed cancer in women. Numerous studies have previously verified the important role of long non-coding RNAs in a number of biological processes in BC. In the present study, analysis of The Cancer Genome Atlas database and reverse transcription-quantitative PCR demonstrated that LOC102724163 expression levels were significantly upregulated in BC tissues compared to matched adjacent normal tissues and were associated with an unfavorable prognosis in patients with BC. Gain or loss of function assays indicated that overexpression of LOC102724163 significantly increased tumorgenicity in vivo and cell migration, proliferation and invasion in vitro . In the mechanistical aspect, LOC102724163 sponged microRNA (miR)-508-5p to elevate MUC19 expression. Additionally, rescue assays ascertained the function of the LOC102724163 /miR-508-5p/ MUC19 axis in the proliferation and invasion of BC cells. To the best of our knowledge, this is the first study to have demonstrated that LOC10272416 3 may act as a competing endogenous RNA to control MUC19 expression levels by competitively sponging miR-508-5p to modulate BC progression. Therefore, the present study has provided new insights into BC diagnosis and treatment.

LncRNA HOTTIP promotes inflammatory response in acute gouty arthritis via miR‐101‐3p/BRD4 axis

“…TRIzol was eluted and RNA from the nucleus and plasma was extracted. RT‐qPCR was performed to quantify HOTTIP levels in the cytoplasm and nucleus, and U6 and GAPDH were employed as nuclear and cytoplasmic controls 22 …”

Technology-driven energy revolution: the impact of digital technology on energy efficiency and its mechanism

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