Local administration of TGFβ-1/VEGF165 gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits

Wei, Xuelei; Mao, Zebin; Hou, Yu; Lin, Lin; Xue, Tao; Chen, Lianxu; Wang, Haijun; Yu, Chunxiao

doi:10.1016/j.bbrc.2011.02.015

Cited by 65 publications

(62 citation statements)

References 20 publications

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“…(Kruse et al, 2012) These recommendations to patients after ACL reconstruction were mostly based on investigation of ACL reconstruction healing using animal models. For example, the ACL graft properties were shown to sharply deteriorate after surgery until 6 to 8 weeks and then slowly to recover (Wei et al, 2011). Therefore, most postoperative knee kinematics research did not consider the postoperative time effect.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that ACL reconstruction experiences postoperative graft healing/remodeling as was shown in animal model (Wei et al, 2011) as well as muscle strength recovery through rehabilitations (Grindem et al, 2014; Grindem et al, 2015). A paucity of data and inconsistencies exists in the current literature when it comes to changes of knee joint kinematics with postoperative time.…”

Section: Introductionsmentioning

confidence: 99%

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Postoperative time dependent tibiofemoral articular cartilage contact kinematics during step-up after ACL reconstruction

Lin

Kernkamp

et al. 2016

Journal of Biomechanics

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This study was to investigate the in vivo tibiofemoral cartilage contact locations before and after anterior cruciate ligament (ACL) reconstruction at 6 and 36 months. Ten patients with unilateral ACL injury were included. A step-up motion was analyzed using a combined magnetic resonance modeling and dual fluoroscopic imaging techniques. The preoperative (i.e. ACL deficient and healthy contralateral) and postoperative cartilage contact locations at 6 and 36 months were analyzed. Similar patterns of the cartilage contact locations during the step-up motion were found for the preoperative and postoperative knee states as compared to the preoperative healthy contralateral side. At the end of step-up motion, the medial contact locations at postoperative 36 months were more anterior when compared to the preoperative healthy contralateral (p=0.02) and 6 months postoperative knee states (p=0.01). The changes of the cartilage contact locations at 36 months after ACL reconstruction compared to the healthy contralateral side were strongly correlated with the changes at 6 months postoperatively. This study showed that the tibiofemoral cartilage contact locations of the knee changes with time after ACL reconstruction, implying an ongoing recovery process within the 36 months after the surgery. There could be an association between the short-term (6 months) and longer-term (36 months) contact kinematics after ACL reconstruction. Future studies need to investigate the intrinsic relationship between knee kinematics at different times after ACL reconstruction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionsmentioning

confidence: 99%

Postoperative time dependent tibiofemoral articular cartilage contact kinematics during step-up after ACL reconstruction

Lin

Kernkamp

et al. 2016

Journal of Biomechanics

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“…Animal models have been used to evaluate exogenous application of specific growth factors including transforming growth factor beta (TGF-β, epidermal growth factor (EGF), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) [53,54,56,59,60]. PDGF and the combination of TGF-β and EGF have been shown to improve the structural properties of the healing graft in the early stages of healing [54,56].…”

Section: Discussionmentioning

confidence: 99%

Increased platelet concentration does not improve functional graft healing in bio-enhanced ACL reconstruction

Fleming

Proffen

Vavken

et al. 2014

Knee Surg Sports Traumatol Arthrosc

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Purpose The use of an extra-cellular matrix scaffold (ECM) combined with platelets to enhance healing of an ACL graft (“bio-enhanced ACL reconstruction”) has shown promise in animal models. However, the effects of platelet concentration on graft healing remains unknown. The objectives of this study were to determine if increasing the platelet concentration in the ECM scaffold would; 1) improve the graft biomechanical properties, and 2) decrease cartilage damage after surgery. Methods Fifty-five adolescent minipigs were randomized to 5 treatment groups; untreated ACL transection (n=10), conventional ACL reconstruction (n=15), and bio-enhanced ACL reconstruction using 1X (n=10), 3X (n=10) or 5X (n=10) platelet-rich plasma. The graft biomechanical properties, anteroposterior (AP) knee laxity, graft histology and macroscopic cartilage integrity were measured at 15 weeks. Results The mean linear stiffness of the bio-enhanced ACL reconstruction procedure using the 1X preparation was significantly greater than traditional reconstruction while the 3X and 5X preparations were not. The failure loads of all the ACL reconstructed groups were equivalent but significantly greater than untreated ACL transection. There were no significant differences in the ligament maturity index or AP laxity between reconstructed knees. Macroscopic cartilage damage was relatively minor, though significantly less when the ECM-platelet composite was used. Conclusions Only the 1X platelet concentration improved healing over traditional ACL reconstruction. Increasing the platelet concentration from 1X to 5X in the ECM scaffold did not further improve the graft mechanical properties. The use of an ECM-platelet composite decreased the amount of cartilage damage seen after ACL surgery.

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“…Sustaining sufficient quantities of growth factor within the local tissue has been difficult until the introduction of gene transfer technology [21]. Wei et al experimented by transfecting bone-marrow-derived MSCs with adenovirus vector encoding TGF- β 1, VEGF, or TGF- β 1/VEGF before surgical implantation into experimental ACL grafts [24]. They found that this combination significantly improved the performance of the MSCs by promoting angiogenesis.…”

Section: Mesenchymal Stem Cells In Ligament Tissue Engineeringmentioning

confidence: 99%

Ligament Tissue Engineering and Its Potential Role in Anterior Cruciate Ligament Reconstruction

Yates

Rupani

Foley

et al. 2012

Stem Cells International

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Tissue engineering is an emerging discipline that combines the principle of science and engineering. It offers an unlimited source of natural tissue substitutes and by using appropriate cells, biomimetic scaffolds, and advanced bioreactors, it is possible that tissue engineering could be implemented in the repair and regeneration of tissue such as bone, cartilage, tendon, and ligament. Whilst repair and regeneration of ligament tissue has been demonstrated in animal studies, further research is needed to improve the biomechanical properties of the engineered ligament if it is to play an important part in the future of human ligament reconstruction surgery. We evaluate the current literature on ligament tissue engineering and its role in anterior cruciate ligament reconstruction.

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Local administration of TGFβ-1/VEGF165 gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits

Cited by 65 publications

References 20 publications

Postoperative time dependent tibiofemoral articular cartilage contact kinematics during step-up after ACL reconstruction

Postoperative time dependent tibiofemoral articular cartilage contact kinematics during step-up after ACL reconstruction

Increased platelet concentration does not improve functional graft healing in bio-enhanced ACL reconstruction

Ligament Tissue Engineering and Its Potential Role in Anterior Cruciate Ligament Reconstruction

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