Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model

Abstract

Background Osteonecrosis of the femoral head (ONFH) is a refractory disease due to its unclear pathomechanism. Neither conservative treatment nor surgical treatment during the early stage of ONFH achieves satisfactory results. Therefore, this study aims to explore the available evidence on the effect of zoledronic acid on early-stage ONFH. Methods For groups were established:the Normal group, model group, Normal saline group(NS group) and zoledronic acid-treated group . The blood supply to the femoral head of animals in the model group and zoledronic acid-treated group was interrupted via a surgical procedure, and zoledronic acid was then locally administered to the femoral head. Four weeks after surgery, all the hips were harvested and evaluated by micro-CT and histopathology(H&E staining, TRAP staining, Toluidine blue staining and masson staining). Results The values of BMD, BS/BV and Tb.Th in the Normal group and zoledronic acid-treated group were significantly higher than those in the model group and NS group (p ​< ​0.05). The outcome of H&E staining, Toluidine blue staining and masson staining were consistent with that of micro-CT. Conclusion The local administration of zoledronic acid in the femoral head had positive effects on the bone structure of the femoral head in a modified rat model of traumatic ONFH and offered a promising therapeutic strategy during the early stage of ONFH. The Translational potential of this article This article could provide a choice for treating patients who have osteonecrosis of femora head and can be the basic research for advanced development over this disease

Therapeutic effect of human umbilical cord mesenchymal stem cells in early traumatic osteonecrosis of the femoral head

“…Second, sufficient HUCMSCs were injected into the femoral head through local injection. Since the volume of the rat femoral head is approximately 14 ​μl after calculation [ 13 ], we injected approximately 100 ​μl into each femoral head, so 14 ​μl of HUCMSC suspension could be retained locally. The survival of HUCMSCs was observed at 4 and 8 weeks after the operation to evaluate the therapeutic effect.…”

“…We adopted a model of traumatic ONFH [ 13 ]. All thirty-six rats were anesthetized by injecting 2% pentobarbital (0.3 ml/100 ​g) into the abdominal cavity; then, the rats were placed on the operating table.…”

Emerging roles of growth factors in osteonecrosis of the femoral head

“…Bisphosphonates are potent inhibitors of osteoclast-mediated bone resorption, which inhibit osteoclasts and reduce femoral head deformities ( Ma et al, 2021 ). Local administration of zoledronic acid in the femoral head has positive effects on the bone structure of the femoral head in modified rat models of traumatic ONFH ( Zhao J. et al, 2021 ). Although monotherapy with bisphosphonates can maintain the structural integrity of the necrotic epiphysis, the lack of new bone formation has been observed in studies on large animal models ( Kim et al, 2005 ).…”

Fat Extract Modulates Calcium Signaling and Protects Against Hyperactive Osteoclastogenesis in Bone Remodeling with Antioxidant Capacity

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