Local and systemic effects on blood lactate concentration during exercise with small and large muscle groups

Chudalla, Robin; Baerwalde, Sven; Schneider, Gerald; Maassen, Norbert

doi:10.1007/s00424-006-0082-5

Cited by 14 publications

(11 citation statements)

References 46 publications

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“…Following centrifugation at 1,500 g for 10 min (4°C), resulting plasma samples were frozen and stored at Ϫ80°C for later analysis. Plasma epinephrine and norepinephrine were determined by highpressure liquid chromatography (Clinrep complete kit for catecholamines, Recipe Chemicals, Munich, Germany) as previously described (5). Plasma cortisol was determined in duplicate in heparinized plasma by ELISA (DRG Diagnostics, Marlburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Effect of exercise with and without a thermal clamp on the plasma heat shock protein 72 response

Whitham¹,

Laing²,

Jackson³

et al. 2007

Journal of Applied Physiology

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The contribution of heat and exercise related stress to the release of heat shock protein 72 (HSP72) is currently unknown. The purpose of the present study was to determine the combined and independent effects of heat and exercise on the extracellular (e)HSP72 response. Eleven moderately trained male volunteers [means +/- SD: age 21 +/- 4 yr; body mass 75.7 +/- 7.7 kg; maximal oxygen uptake ((.)Vo(2 max)) 57.8 +/- 3.3 ml.kg(-1).min(-1)] completed four 2-h, heat-manipulated, water-immersion trials. Trials were exercise-induced heat (EIH; rectal temperature change +2.2 degrees C), clamped exercise (CEx; 0 degrees C), passive heating (PHT; +2.3 degrees C), and control (Con; 0 degrees C). Exercise trials (EIH and CEx) comprised deep-water running at 58.5 +/- 2.4 and 59.1 +/- 1.7% (.)vo(2)max. eHSP72 and catecholamine concentrations were determined by ELISA and HPLC, respectively, pre- and postimmersion. All trials induced an eHSP72 response (P < 0.05) with postimmersion values significantly greater on EIH compared with other trials (6.0 +/- 3.4; CEx 3.8 +/- 2.6; PHT 2.7 +/- 2.1; Con 2.2 +/- 1.9 ng/ml). Exercising with a thermal clamp blunted the eHSP72 response, but postimmersion values were also greater than Con. PHT induced a large catecholamine response, but postimmersion eHSP72 values did not reach significance vs. Con. Given that exercising with a thermal clamp evoked a significant increase in plasma eHSP72 concentration, exercise-related stressors other than heat appeared influential in stimulating HSP72 release. Moreover, the catecholamine data from PHT suggest neither epinephrine nor norepinephrine was solely responsible for eHSP72 release.

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Section: Methodsmentioning

confidence: 99%

Effect of exercise with and without a thermal clamp on the plasma heat shock protein 72 response

Whitham¹,

Laing²,

Jackson³

et al. 2007

Journal of Applied Physiology

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“…Aliquots of heparinized plasma were used for the determination of cortisol and G-CSF (DRG Diagnostics, Marlburg, Germany, and R & D Systems, Oxford, UK, respectively). Aliquots of EDTA plasma were used for the determination of epinephrine and norepinephrine using high-pressure liquid chromatography (Clinrep complete kit for catecholamines, Recipe Chemicals, Munich, Germany) as described previously (8). Due to limited sample volume, epinephrine and norepinephrine were analyzed on N ϭ 9.…”

Section: Subjectsmentioning

confidence: 99%

Human blood neutrophil responses to prolonged exercise with and without a thermal clamp

Laing¹,

Jackson²,

Walters³

et al. 2008

Journal of Applied Physiology

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The purpose of this study was to investigate the effects of prolonged exercise with and without a thermal clamp on neutrophil trafficking, bacterial-stimulated neutrophil degranulation, stress hormones, and cytokine responses. Thirteen healthy male volunteers (means +/- SE: age 21 +/- 1 yr; mass 74.9 +/- 2.1 kg; maximal oxygen uptake 58 +/- 1 ml x kg(-1) x min(-1)) completed four randomly assigned, 2-h water-immersion trials separated by 7 days. Trials were exercise-induced heating (EX-H: water temperature 36 degrees C), exercise with a thermal clamp (EX-C: 24 degrees C), passive heating (PA-H: 38.5 degrees C), and control (CON: 35 degrees C). EX-H and EX-C was comprised of 2 h of deep water running at 58% maximal oxygen uptake. Blood samples were collected at pre-, post-, and 1 h postimmersion. Core body temperature was unaltered on CON, clamped on EX-C (-0.02 degrees C), and rose by 2.23 degrees C and 2.31 degrees C on EX-H and PA-H, respectively. Exercising with a thermal clamp did not blunt the neutrophilia postexercise (EX-C postexercise: 9.6 +/- 1.1 and EX-H postexercise: 9.8 +/- 1.0 x 10(9)/liter). Neutrophil degranulation decreased (P < 0.01) similarly immediately after PA-H (-21%), EX-C, and EX-H (-28%). EX-C blunted the circulating norepinephrine, cortisol, granulocyte-colony stimulating factor, and IL-6 response (P < 0.01) but not the plasma epinephrine and serum growth hormone response. These results show a similar neutrophilia and decrease in neutrophil degranulation after prolonged exercise with and without a thermal clamp. As such, the rise in core body temperature does not appear to mediate neutrophil trafficking and degranulation responses to prolonged exercise. In addition, these results suggest a limited role for cortisol, granulocyte-colony stimulating factor, and IL-6 in the observed neutrophil responses to prolonged exercise.

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“…O limiar anaeróbio (lactato máximo de equilíbrio), representado pelo valor máximo da estabilização entre produção e remoção do lactato sanguíneo (7,8) , é considerado um importante indicador da capacidade de exercício aeróbio e reflete a intensidade do exercício (14) . Os dados do presente estudo demonstraram que os animais sedentários atingiram essa estabilização durante o teste de esforço sem sobrecarga a uma concentração de ~6 mmol/L (Figura 1), concentração semelhante à encontrada por outros (2,3,10) .…”

Section: Discussionunclassified

Treinamento aeróbio em natação melhora a resposta de parâmetros metabólicos de ratos durante teste de esforço

Freitas

Carneiro‐Júnior

Franco

et al. 2010

Rev Bras Med Esporte

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RESUMOForam investigados os efeitos do treinamento aeróbio em natação com baixa intensidade sobre as respostas do lactato e da glicose sanguíneos de ratos durante teste de esforço. Ratos Wistar adultos foram distribuídos aleatoriamente em dois grupos: sedentário (n = 6) e treinado (n = 6). Todos receberam água e ração ad libitum e foram mantidos em ambiente com temperatura de 22 ± 2ºC e ciclo claro/escuro de 12 horas. O grupo treinado foi submetido a um programa de natação contínua sem sobrecarga, 30 min/dia, cinco dias/semana, por seis semanas. Três dias após a última sessão de treino, as concentrações sanguíneas de lactato e glicose foram medidas em três momentos durante dois testes de esforço de 20 minutos (repouso, 10 min e 20 min), sendo um sem carga e outro com carga (5% do peso corporal), separados por dois dias. Observou-se correlação inversa entre lactato e glicose durante o exercício (ρ = -0,74; P < 0,001). A concentração de lactato elevou-se do repouso para 10 min (P < 0,05) e estabilizouse entre 10 e 20 min, em ambos os grupos nos dois testes. No teste com carga, o lactato estabilizou-se em níveis mais elevados frente aos níveis sem carga (P < 0,05), nos dois grupos. Os animais treinados exibiram níveis de lactato mais baixos do que os sedentários (P < 0,05) nos dois testes. A glicose sanguínea decaiu do repouso até 20 min nos sedentários, no teste com carga (P < 0,05). Nos treinados, a glicose sanguínea estabilizou-se em ambos os testes (P > 0,05). Conclui-se que o treinamento aeróbio em natação aplicado foi capaz de alterar as respostas do lactato e glicose sanguíneos de ratos durante os testes de esforço.Palavras-chave: atividade física, limiar anaeróbio, lactato, glicose. ABSTRACTThe effects of low intensity aerobic swimming training on blood lactate and glucose responses in rats were investigated during exertion test. Twelve adult male Wistar rats were randomly divided into two groups: sedentary (n= 6) and trained (n= 6). All animals received water and food ad libitum and were kept in a room with temperature of 22 ± 2ºC and dark/light cycle of 12 hours. Animals from trained group were submitted to a swimming training protocol of 30 min/day, 5 days/week, for 6 weeks. Sedentary animals did not exercise. Three days after the last training session all animals were submitted to two 20-minute swimming tests with 48 hour-interval, being one unloaded and the other with a load of 5 % of body weight. Blood lactate and glucose were measured at rest, 10 min and 20 min of exercise. Negative correlation between blood lactate and glucose levels was observed during the exertion tests (ρ = -0.74, P<0.001). Blood lactate concentration increased from rest to 10 min of exercise and stabilized from 10 to 20 min of exercise in both exercised and sedentary animals (P<0.05) during the unloaded exertion test. Blood lactate stabilized at higher levels when compared to those in the unloaded test in both groups (P<0.05) during the loaded exertion test. Trained rats presented lower levels of blood lactate than sedentary ani...

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Local and systemic effects on blood lactate concentration during exercise with small and large muscle groups

Cited by 14 publications

References 46 publications

Effect of exercise with and without a thermal clamp on the plasma heat shock protein 72 response

Effect of exercise with and without a thermal clamp on the plasma heat shock protein 72 response

Human blood neutrophil responses to prolonged exercise with and without a thermal clamp

Treinamento aeróbio em natação melhora a resposta de parâmetros metabólicos de ratos durante teste de esforço

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