Local and Systemic Expression of Insulin-like Growth Factor-I (IGF-I) mRNAs in Rat after Bone Marrow Ablation

Tanaka, Hiroshi; Wakisaka, Atsuhiko; Ogasa, Hiroyoshi; Kawai, Shinya; Liang, Chunyu

doi:10.1006/bbrc.2001.5711

Cited by 12 publications

(10 citation statements)

References 31 publications

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“…Mechanical bone marrow ablation (BMX) is a very useful experimental model for examining the sequence and mechanism of events involved in osteogenesis and bone turnover within a defined time interval. (8)(9)(10)(11)(12)(13)(14)(15) Mechanical BMX provokes a predictable sequence of events, including the formation of a blood clot with inflammation and capillary invasion, mesenchymal cell migration, osteoblast proliferation, and formation of new cancellous medullary bone, followed by osteoclastic bone resorption and bone marrow reconstitution. (16)(17)(18)(19) Previous studies have employed this model to investigate the effect of exogenous PTH on the formation of new bone that forms after marrow ablation.…”

Section: Introductionmentioning

confidence: 99%

Endogenous parathyroid hormone–related protein compensates for the absence of parathyroid hormone in promoting bone accrual in vivo in a model of bone marrow ablation

Zhu

Zhou

Zhu

et al. 2013

Journal of Bone and Mineral Research

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To assess the effect of hypoparathyroidism on osteogenesis and bone turnover in vivo, bone marrow ablation (BMXs) were performed in tibias of 8-week-old wild-type and parathyroid hormone-null (PTH À/À ) mice and newly formed bone tissue was analyzed from 5 days to 3 weeks after BMX. At 1 week after BMX, trabecular bone volume, osteoblast numbers, alkaline phosphatase-positive areas, type I collagen-positive areas, PTH receptor-positive areas, calcium sensing receptor-positive areas, and expression of bone formation-related genes were all decreased significantly in the diaphyseal regions of bones of PTH À/À mice compared to wild-type mice. In contrast, by 2 weeks after BMX, all parameters related to osteoblastic bone accrual were increased significantly in PTH À/À mice. At 5 days after BMX, active tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts had appeared in wild-type mice but were undetectable in PTH À/À mice, Both the ratio of mRNA levels of receptor activator of NF-kB ligand (RANKL)/osteoprotegerin (OPG) and TRAP-positive osteoclast surface were still reduced in PTH À/À mice at 1 week but were increased by 2 weeks after BMX. The expression levels of parathyroid hormone-related protein (PTHrP) at both mRNA and protein levels were upregulated significantly at 1 week and more dramatically at 2 weeks after BMX in PTH À/À mice. To determine whether the increased newly formed bones in PTH À/À mice at 2 weeks after BMX resulted from the compensatory action of PTHrP, PTH À/À PTHrP þ/À mice were generated and newly formed bone tissue was compared in these mice with PTH À/À and wild-type mice at 2 weeks after BMX. All parameters related to osteoblastic bone formation and osteoclastic bone resorption were reduced significantly in PTH À/À PTHrP þ/À mice compared to PTH À/À mice. These results demonstrate that PTH deficiency itself impairs osteogenesis, osteoclastogenesis, and osteoclastic bone resorption, whereas subsequent upregulation of PTHrP in osteogenic cells compensates by increasing bone accrual.

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Section: Introductionmentioning

confidence: 99%

Endogenous parathyroid hormone–related protein compensates for the absence of parathyroid hormone in promoting bone accrual in vivo in a model of bone marrow ablation

Zhu

Zhou

Zhu

et al. 2013

Journal of Bone and Mineral Research

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“…[33][34][35][36][37] Logically, these phases are driven by a sequential cascade of gene expression. 35,36,[38][39][40][41] In the rat femoral implant model, the basic regenerative cycle is similar to the marrow ablation model in which the surgical injury induces medullary bone formation within 2 weeks, followed by a period of bone remodeling during which some of the medullary bone is resorbed; however, bone is left in contact with the implant. 42,43 A number of groups are now using an adaptation of this model to examine issues related to implant fixation, with the main focus on implant surface treatments.…”

Section: Introductionmentioning

confidence: 99%

Peri‐implant bone formation and implant integration strength of peptide‐modified p(AAM‐co‐EG/AAC) interpenetrating polymer network‐coated titanium implants

Barber

Ranieri

et al. 2006

J Biomedical Materials Res

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Interpenetrating polymer networks (IPNs) of poly (acrylamide-co-ethylene glycol/acrylic acid) functionalized with an -Arg-Gly-Asp- (RGD) containing 15 amino acid peptides, derived from rat bone sialoprotein (bsp-RGD(15), were grafted to titanium implants in an effort to modulate bone formation in the peri-implant region in the rat femoral ablation model. Bone-implant contact (BIC) and bone formation within the medullary canal were determined using microcomputed tomography at 2 and 4 weeks postimplantation. BIC for bsp-RGD(15)-IPN implants was enhanced relative to hydroxyapatite tricalcium phosphate (HA-TCP) coated implants, but was similar to all other groups. Aggregate bone formation neither indicated a dose-dependent effect of bsp-RGD(15) nor a meaningful trend. Mechanical testing of implant fixation revealed that only the HA-TCP coated implants supported significant (>1 MPa) interfacial shear strength, despite exhibiting lower overall BIC, an indication that bone ingrowth into the rougher coating was the primary mode of implant fixation. While no evidence was found to support the hypothesis that bsp-RGD(15)-modified IPN coated implants significantly impacted bone-implant bonding, these results point to the lack of correlation between in vitro studies employing primary osteoblasts and in vivo wound healing in the peri-implant region.

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“…Mechanical BMX provokes a predictable sequence of events, including the formation of a blood clot with inflammation and capillary invasion, mesenchymal cell migration, osteoblast proliferation, and formation of new cancellous medullary bone, followed by osteoclastic bone resorption and bone marrow reconstitution [12][13][14][15]. Studies have employed this model to investigate the effect of identifying genes involved in osteogenesis and determining their roles in bone turnover [6][7][8][9][10][11][16][17][18]. Therefore, we believe that the combined ovariectomized mouse model with mechanical BMX approaches will be helpful to explore the effect of estrogen deficiency on osteogenesis and bone turnover in vivo.…”

mentioning

confidence: 99%

Bone marrow ablation demonstrates that estrogen plays an important role in osteogenesis and bone turnover via an antioxidative mechanism

Shi

Yan

et al. 2015

Bone

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Local and Systemic Expression of Insulin-like Growth Factor-I (IGF-I) mRNAs in Rat after Bone Marrow Ablation

Cited by 12 publications

References 31 publications

Endogenous parathyroid hormone–related protein compensates for the absence of parathyroid hormone in promoting bone accrual in vivo in a model of bone marrow ablation

Endogenous parathyroid hormone–related protein compensates for the absence of parathyroid hormone in promoting bone accrual in vivo in a model of bone marrow ablation

Peri‐implant bone formation and implant integration strength of peptide‐modified p(AAM‐co‐EG/AAC) interpenetrating polymer network‐coated titanium implants

Bone marrow ablation demonstrates that estrogen plays an important role in osteogenesis and bone turnover via an antioxidative mechanism

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