Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation

Buck, Kenneth B.; Schaefer, A.; Schoonderwoert, Vincent Th. G.; Creamer, Matthew S.; Dufresne, Eric R.; Forscher, Paul

doi:10.1091/mbc.e16-04-0228

Cited by 8 publications

(11 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the integrin-activating adaptor protein kindlin-2 (Fermt2) interacts with paxillin to activate Rac1 and associates with the Arp2/3 complex to induce membrane protrusions ( Böttcher et al, 2017 ). Local Arp2/3-dependent actin polymerisation may thus regulate efficient traction force application, buffering nascent adhesions from the mechanical effects of retrograde flow ( Buck et al, 2017 ). Arp2/3-depleted fibroblasts failed to properly align focal adhesions ( Wu et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts

et al. 2020

View full text Add to dashboard Cite

The Arp2/3 complex is essential for the assembly of branched filamentous actin but its role in physiology and development is surprisingly little understood. Melanoblasts deriving from the neural crest migrate along the developing embryo and traverse the dermis to reach the epidermis colonising the skin and eventually homing within the hair follicles. We have previously established that Rac1 and Cdc42 direct melanoblast migration in vivo. We hypothesised that the Arp2/3 complex might be the main downstream effector of these small GTPases. Arp3 depletion in the melanocyte lineage results in severe pigmentation defects in dorsal and ventral regions of the mouse skin. Arp3 null melanoblasts demonstrate proliferation and migration defects and fail to elongate as their wild-type counterparts. Conditional deletion of Arp3 in primary melanocytes causes improper proliferation, spreading, migration and adhesion to extracellular matrix. Collectively, our results suggest that the Arp2/3 complex is absolutely indispensable in the melanocyte lineage in mouse development, and indicate a significant role in developmental processes that require tight regulation of actin-mediated motility.

show abstract

Section: Discussionmentioning

confidence: 99%

The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts

et al. 2020

View full text Add to dashboard Cite

show abstract

“…73 However, a reasonable prediction is that different ECM pre-gels, even below gelation concentrations, could have distinct mechanical and physical compositions that could regulate growth. 74 Mechanical or physical changes that alter substrate adhesions patterns, 75 adhesion signaling, 76 mechanical forces on adhesion sites, 77 and/or the traction forces generated between substrate adhesions and the cytoskeleton 78 would all be expected to alter neurite growth and warrant further study. During ECM injection, ECM factors can penetrate CNS tissues prior to gelation, exposing neurons and other cells to both soluble and insoluble ECM factors.…”

Section: Discussionmentioning

confidence: 99%

Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching

et al. 2017

View full text Add to dashboard Cite

Central nervous system neurons often degenerate after trauma due to the inflammatory innate immune response to injury, which can lead to neuronal cell death, scarring, and permanently lost neurologic function. Extracellular matrix bioscaffolds, derived by decellularizing healthy tissues, have been widely used in both preclinical and clinical studies to promote positive tissue remodeling, including neurogenesis, in numerous tissues, with extracellular matrix from homologous tissues often inducing more positive responses. Extracellular matrix hydrogels are liquid at room temperature and enable minimally invasive extracellular matrix injections into central nervous system tissues, before gelation at 37℃. However, few studies have analyzed how extracellular matrix hydrogels influence primary central nervous system neuron survival and growth, and whether central nervous system and non-central nervous system extracellular matrix specificity is critical to neuronal responses. Urinary bladder extracellular matrix hydrogels increase both primary hippocampal neuron survival and neurite growth to similar or even greater extents, suggesting extracellular matrix from non-homologous tissue sources, such as urinary bladder matrix-extracellular matrix, may be a more economical and safer alternative to developing central nervous system extracellular matrices for central nervous system applications. Additionally, we show matrix-bound vesicles derived from urinary bladder extracellular matrix are endocytosed by hippocampal neurons and positively regulate primary hippocampal neuron neurite growth. Matrix-bound vesicles carry protein and RNA cargos, including noncoding RNAs and miRNAs that map to the human genome and are known to regulate cellular processes. Thus, urinary bladder matrix-bound vesicles provide natural and transfectable cargoes which offer new experimental tools and therapeutic applications to study and treat central nervous system neuron injury.

show abstract

“…The third subtype of F-actin structures are the transverse actin arcs that surround the central domain and control its shape as well as the distribution of MTs ( Schaefer et al, 2002 , 2008 ; Zhang et al, 2003 ). Lastly, the fourth F-actin structure is the dynamically rearranging intrapodia or ruffles in the transition zone ( Rochlin et al, 1999 ), which recently have been suggested to promote traction force generation by buffering developing adhesion sites from the effects of retrograde flow ( Buck et al, 2017 ).…”

Section: Neuronal Structurementioning

confidence: 99%

An Integrated Cytoskeletal Model of Neurite Outgrowth

Miller

Suter

2018

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Neurite outgrowth underlies the wiring of the nervous system during development and regeneration. Despite a significant body of research, the underlying cytoskeletal mechanics of growth and guidance are not fully understood, and the relative contributions of individual cytoskeletal processes to neurite growth are controversial. Here, we review the structural organization and biophysical properties of neurons to make a semi-quantitative comparison of the relative contributions of different processes to neurite growth. From this, we develop the idea that neurons are active fluids, which generate strong contractile forces in the growth cone and weaker contractile forces along the axon. As a result of subcellular gradients in forces and material properties, actin flows rapidly rearward in the growth cone periphery, and microtubules flow forward in bulk along the axon. With this framework, an integrated model of neurite outgrowth is proposed that hopefully will guide new approaches to stimulate neuronal growth.

show abstract

Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation

Cited by 8 publications

References 73 publications

The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts

The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts

Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching

An Integrated Cytoskeletal Model of Neurite Outgrowth

Contact Info

Product

Resources

About