Local Auxin Biosynthesis Is a Key Regulator of Plant Development

Brumós, Javier; Robles, Linda M.; Yun, Jeonga; Vu, Thien; Jackson, Savannah; Alonso, José M.; Stepanova, Anna N.

doi:10.1016/j.devcel.2018.09.022

Cited by 263 publications

(207 citation statements)

References 66 publications

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…On the other hand, gibberellins signalling are widely manipulated by the proteolytic activity of DELLA proteins (Xiang et al, 2018). Most of the interacting plant microbes supress the proteolysis of DELLA proteins in host plants by secreting a variety of compounds (Brumos et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Yousaf

Hussain

Hamayun

et al. 2019

Preprint

View full text Add to dashboard Cite

16Besides acting as growth inducing molecule, Gibberellin (GA 3 ) also confers the compatibility 17 of microbial interactions with host. We inoculated 11 days old Z. mays seedlings grown under 18 hydroponic conditions and high GA 3 levels with Bipolaris sorokiniana (BIPOL) at the spore 19 density (SD) of OD 0.6 . The high level of GA 3 negatively affected the growth of the seedlings, 20 accompanied by the high level of stress deducing secondary metabolites (proline, total 21 flavanoids, phenylpropanoids, and glucosinolides). Moreover, high level of GA 3 produced a 22 hypersensitive response (HR) in the seedlings. The HR developed cross talks with IAA and 23 trans-zeatins and triggered higher production of hypersensitive inducing biomolecules. The 24 other HR co-related biological processes were demonstrated by high phytoalexins level and 25 high protease activities. Such activities ultimately inhibited the colonization of BIPOL on the 26 roots of maize seedlings. The products of the genes expressed at high GA 3 also conferred the 27 deterrence of BIPOL colonization at SD = OD 0.6 . Intriguingly, when we inhibited GA 3 28 biosynthesis in the seedlings with aerially sprayed uniconizole, prior to BIPOL treatment, the 29 BIPOL colonized and subsequently promoted the seedling growth. This low level of GA 3 30 after BIPOL treatment checked the high level of secondary metabolites and hypersensitivity 31 inducing molecules. The results, thus suggested that the aforementioned processes only 32 happened in the BIPOL at SD (OD 0.6 ), whereas the SD at lower levels (OD 0.2 or OD 0.4 ) 33 neither promoted the growth of uniconizole pre-treated seedlings nor produced HR in control 34 seedlings of maize plant.35

show abstract

Section: Introductionmentioning

confidence: 99%

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Yousaf

Hussain

Hamayun

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…6e-f). In summary, PDK1 deficiency leads to a plethora of defects in auxin-regulated organogenesis processes and tropic responses, resembling those mutants defective in the biosynthesis, transport or signalling of auxin 3, 38, 39 .…”

Section: Resultsmentioning

confidence: 90%

A lipid code-dependent phosphoswitch directs PIN-mediated auxin efflux in Arabidopsis development

Tan

Zhang

Kong

et al. 2019

Preprint

View full text Add to dashboard Cite

18 19 Directional intercellular transport of the phytohormone auxin mediated by PIN FORMED (PIN) 20 efflux carriers plays essential roles in both coordinating patterning processes and integrating 21 multiple external cues by rapidly redirecting auxin fluxes. Multilevel regulations of PIN activity 22 under internal and external cues are complicated; however, the underlying molecular mechanism 23 remains elusive. Here we demonstrate that 3'-Phosphoinositide-Dependent Protein Kinase1 24 (PDK1), which is conserved in plants and mammals, functions as a molecular hub integrating 25 the upstream lipid signalling and the downstream substrate activity through phosphorylation. 26 Genetic analysis uncovers that loss-of-function Arabidopsis mutant pdk1.1 pdk1.2 exhibits a 27 plethora of abnormalities in organogenesis and growth, due to the defective PIN-dependent 28 auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 29 Protein Kinase to facilitate its activity towards PIN proteins. Our studies establish a lipid-30 dependent phosphorylation cascade connecting membrane composition-based cellular signalling 31 with plant growth and patterning by regulating morphogenetic auxin fluxes. 32 33 34 Due to a sessile lifestyle, plants have evolved strict developmental programming as well as 35 adaptive plasticity in response to diverse environmental stimuli, which is ensured by a 36 framework of multiple signalling pathways. The phytohormone auxin (indole-3-acetic acid, IAA) 37 plays a pivotal role in shaping plant growth and development, in a concentration-dependent 38 manner. The establishment and maintenance of local auxin minima, maxima and gradients rely 39 on directional intercellular transport, which is mediated by a subset of auxin influx carriers 40 AUXIN1 (AUX1)/LIKE AUX1s (LAXs) 1 , PIN (PIN-FORMED) efflux carriers 2,3 and ABCB-41 type transporters 4 . It has been well documented that the activity and polar localization of PIN 42 proteins are regulated by intracellular trafficking as well as post-translational modifications, 43 including reversible phosphorylation 5 . 44 PINs are phosphorylated at multiple serine/threonine residues by various protein kinases, 45 including PINOID (PID)/WAVY ROOT GROWTHs (WAGs) 6-8 , D6 PROTEIN KINASEs 46 (D6PK/D6PKL1~3, hereafter as D6PKs) 9,10 , PROTEIN KINASE ASSOCIATED WITH BRX 47 (PAX) 11 , MITOGEN PROTEIN KINASE3/6 (MPK3/6) 12 and CDPK-RELATED KINASE5 48 3 (CRK5) 13 , and dephosphorylated by Protein Phosphatase 2A (PP2A) 8,14 , Protein Phosphatase 6 49 (PP6) 15 and PP1 16 . PID-dependent phosphorylation and PP2A-mediated dephosphorylation 50 antagonize with each other 6,14 , acting as a binary switch determining the apical-basal targeting 51 of PIN proteins. More PID kinase activity results in more apical localization of PINs; conversely, 52 less PIN phosphorylation by PID gives rise to the reduced apical (or more basal) localization. In 53 contrast to PID, D6PKs and PAX phosphorylate basal-localized PINs to regulate their activity 17 . 54To dat...

show abstract

“…Although the IPyA pathway is thought to be the main route of auxin synthesis in plants (Stepanova et al, 2011; Mashiguchi et al, 2011; Brumos et al, 2018), alternative branches may contribute to total IAA pools at least at some developmental stages, or in some tissues, or under specific environmental conditions. Loss-of-function mutations in several of the components of the parallel indole glucosinolate (IG) biosynthetic pathway, specifically SUR2 (also known as CYP83B1 , RED1, RNT1 and ATR4 ) , RTY ( SUR1, ALF1, IVR , and HLS3 ) and UGT74B1 ( WEI9 ), hyper-activate one of the putative IPyA-indepenent auxin biosynthetic branches by re-routing the pathway intermediate indole-3-acetaldoxime (IAOx, that is normally channeled into IG and camalexin production) into the biosynthesis of IAA (Supplemental Fig.…”

Section: Introductionmentioning

confidence: 99%

Structure-function analysis of interallelic complementation inROOTYtransheterozygotes

Brumós

Bobay

Clark

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

AbstractAuxin is a crucial plant growth regulator. Forward genetic screens for auxin-related mutants have led to the identification of key genes involved in auxin biosynthesis, transport, and signaling. Loss-of-function mutations in the genes involved in indole glucosinolate biosynthesis, a metabolically-related route that produces defense compounds from indolic precursors shared with the auxin pathway, result in auxin overproduction. We identified an allelic series of fertile, hypomorphic mutants for an essential indole glucosinolate route gene ROOTY (RTY) that show a range of high-auxin defects. Genetic characterization of these lines uncovered phenotypic suppression by cyp79b2 b3, wei2, and wei7 mutants and revealed the phenomenon of interallelic complementation in several RTY transheterozygotes. Structural modeling of RTY shed light on the structure-to-function relations in the RTY homo- and heterodimers and unveiled the likely structural basis of interallelic complementation. This work underscores the importance of employing true null mutants in genetic complementation studies.

show abstract

Local Auxin Biosynthesis Is a Key Regulator of Plant Development

Cited by 263 publications

References 66 publications

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

A lipid code-dependent phosphoswitch directs PIN-mediated auxin efflux in Arabidopsis development

Structure-function analysis of interallelic complementation inROOTYtransheterozygotes

Contact Info

Product

Resources

About