Local bacillus Calmette-Gu�rin therapy for bovine vulval papilloma and carcinoma

Hill, F. W.; Rutten, Victor P.M.G.; Høyer, Morten; Klein, W. R.; Koten, Jan-Willem; Steerenberg, P. A.; Ruitenberg, E. J.; Otter, W. Den

doi:10.1007/bf01517180

Cancer Immunol Immunother

1994

DOI: 10.1007/bf01517180

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Local bacillus Calmette-Gu�rin therapy for bovine vulval papilloma and carcinoma

F. W. Hill

Victor P.M.G. Rutten

Morten Høyer

et al.

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2024

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Cited by 2 publications

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Analysis of the immunomodulatory properties of mycobacterium cell wall fraction on the cytokine production of peripheral blood mononuclear cells of healthy dogs

Ward,

Wood,

Pye

et al. 2024

Veterinary Dermatology

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BackgroundMycobacterium cell wall fraction (MCWF) is derived from nonpathogenic Mycobacterium phlei and is used as an immunomodulatory compound in clinical practice, yet its mode‐of‐action requires further research.ObjectiveTo evaluate the host response to MCWF in canine peripheral blood mononuclear cells (PBMCs) by using enzyme‐linked immunosorbent assays (ELISA) and quantitative reverse transcription (qRT)‐PCR for assessment of cytokines.AnimalsEight healthy Labrador retrievers.Materials and MethodsPBMCs were isolated from whole blood using density centrifugation. The cells were cultured with different concentrations of MCWF or a potent stimulator of cytokine production, phorbol 12‐myristate 13‐acetate/ionomycin, or left in cell culture medium for 24, 48 and 72 h. Cytokines were measured by ELISA for interleukin (IL)‐4, IL‐10 and interferon‐gamma (IFN‐γ), and by qRT‐PCR for IL‐4, IL‐10, IL‐13, IFN‐γ, tumour necrosis factor alpha (TNF‐α) and transforming growth factor‐beta.ResultsA significant increase of IL‐10 messenger ribonucleic acid (mRNA) was detected at all time points for all concentrations of MCWF (p < 0.05). Protein analysis reflected this finding, with a maximum IL‐10 concentration of 300.6 ± 38.3 μg/mL. Compared to the negative control, post‐stimulation elevation of IFN‐γ mRNA was noted at 24 h with all concentrations of MCWF (p < 0.01), and TNF‐α mRNA was increased for 0.5 μg/dL MCWF only at 72 h (p < 0.05).Conclusions and Clinical RelevanceMCWF stimulation of PBMCs results in the elevation of both proinflammatory and regulatory cytokine mRNA. Further research into the role of MCWF as a systemically administered regulatory immunomodulator or adjuvant to allergen‐specific immunotherapy should be considered.

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Analysis of the immunomodulatory properties of mycobacterium cell wall fraction on the cytokine production of peripheral blood mononuclear cells of healthy dogs

Ward,

Wood,

Pye

et al. 2024

Veterinary Dermatology

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In vitroand Animal Models for Antiviral Therapy in Papillomavirus Infections

Stanley

Masterson

Nicholls

1997

Antivir Chem Chemother

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SummaryThe need for antiviral therapies for papillomavirus infections is well recognized but the difficulties of reproducing the infectious cycle of papillomaviruses in vitro has hindered our understanding of virus-cell interactions and the regulation of viral gene expression during permissive growth. Recent advances in understanding the temporal expression and function of papillomavirus proteins. has enabled consideration of a targeted approach to papillomavirus chemotherapy and in particular the inhibition of viral replication by targeting the El and E2 proteins. There are in vitro culture systems available for the screening of new chemotherapeutic agents, since significant advances have been made with culture systems which promote epithelial differentiation in vitro. However, to date, there are no published data which show that virions generated in vitro can infect keratinocytes and initiate another round of replication in vitro. In vivo animal models are therefore necessary to assess the efficacy of antivirals in preventing and treating viral infection, particularly for the low-risk genital viruses which are on the whole refractory to culture in vitro. Although papillomaviruses affect a wide variety of hosts in a species-specific manner, the animals most useful for modelling papillomavirus infections include the rabbit, ox, mouse, dog, horse, primate and sheep. The ideal animal model should be widely available, easy to house and handle, be large enough to allow for adequate tissue sampling, develop lesions on anatomical sites comparable with those in human diseases and these lesions should be readily accessible for monitoring and ideally should yield large amounts of infectious virus particles for use in both in vivo and in vitro studies. The relative merits of the various papillomavirus animal models available in relation to these criteria are discussed.

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Local bacillus Calmette-Gu�rin therapy for bovine vulval papilloma and carcinoma

Cited by 2 publications

References 11 publications

Analysis of the immunomodulatory properties of mycobacterium cell wall fraction on the cytokine production of peripheral blood mononuclear cells of healthy dogs

Analysis of the immunomodulatory properties of mycobacterium cell wall fraction on the cytokine production of peripheral blood mononuclear cells of healthy dogs

In vitroand Animal Models for Antiviral Therapy in Papillomavirus Infections

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