Local Delivery of Glabridin by Biomolecular Microneedle to Accelerate Infected Wound Healing

Abstract

Applying antibacterial polymers and pro‐regenerative small molecules are two individual strategies for accelerating wound healing. However, integrating those two unique approaches into one therapeutic platform that meets clinical requirements is still a challenge. Herein, a series of antibacterial gelatin methacrylate (GelMA)/ε‐polylysine (ε‐PL) composite hydrogels (termed as GP‐n HGs, n = 0, 10, 20 and 30, respectively) were innovatively fabricated by ultraviolet light (UV) crosslinking. The GP‐n HGs were proved to be broad‐spectrum antibacterial and biocompatible. Among those GP‐n HGs, the GP‐20 HG was selectively processed into microneedle following a mold‐casting method. Then, the glabridin was loaded into those needles to produce composite microneedle termed GP‐20@Gla MN. An S. aureus‐infected full‐thickness defect model in rats was created to evaluate the wound‐healing effect of GP‐20@Gla MN. Furthermore, an RNA sequencing assay was performed to explore the possible molecular mechanisms of glabridin in promoting tissue regeneration, and many positive routes have been summarized. This work is of significant novelty in fulfilling complex clinical needs by simultaneously optimizing the advanced microneedles' chemical compositions and physical structures. This work will provide a promising therapeutic platform for treating infected and chronic wounds.This article is protected by copyright. All rights reserved

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

“…The wound-healing efficacy of GP-20@Gla MN gave positive results in a rat model with a full-thickness defect infected by S. aureus. In addition, an RNA sequencing assay is conducted to examine the glabridin's potential molecular mechanisms in fostering tissue regeneration, revealing various positive pathways [230].…”

Efficient Loading and Sustained Delivery of Methotrexate Using a Tip-Swellable Microneedle Array Patch for Psoriasis Treatment

“…Additionally, the drugs are usually distributed throughout all of the entire MNs. Because of the skin’s elasticity, full insertion of the MNs into the skin is not always possible, potentially leading to drug wastage and imprecise dosage administration. ,− These challenges with variable and unreliable drug loading and delivery efficiency have significantly hindered the clinical application of hydrogel MNs.…”