Local effect of lysophosphatidic acid on prostaglandin production in the bovine oviduct

Abstract

The mammalian oviduct plays an important role in the fertilisation and transport of gametes and embryo. Prostaglandins (PGs) are local mediators of oviductal functions and are involved in fertilisation and the transport of gametes and embryo. Lysophosphatidic acid (LPA), a kind of phospholipid, is involved in various physiological actions. We hypothesised that LPA regulates PG production in the bovine oviduct. To test this hypothesis, we examined the mRNA expression of LPA receptors (LPAR1-6) and LPA-producing enzymes (ATX, PLA1α, PLA1β) in ampullary and isthmic tissues and in cultured epithelial and stromal cells isolated from the bovine oviduct. We also investigated the effects of LPA on PG synthase expression and PG production in cultured cells. The mRNA of LPAR1-4, 6, ATX and PLA1α were expressed in cultured epithelial and stromal cells. The expressions of LPAR1-3 were significantly lower and the expression of LPAR4 was significantly higher in the isthmic than in the ampullary tissues. Lysophosphatidic acid significantly stimulated PG production in the cultured isthmic stromal cells. The overall findings suggest that LPA stimulates PG production via LPAR4 in the bovine oviduct. Since PGs are important for fertilisation and the transport of gametes and embryo, these findings show that locally produced LPA regulates oviductal functions.

<i>In vivo</i> evidence for possible up-regulating roles of lysophosphatidic acid around fertilization in rats

“…A significant immunoreaction of ATX, together with co-existing PLA 2 enzymes in oviductal epithelia, suggests rat oviductal cavity as a site of LPA production and/or secretion. The existence of LPA producing machinery is partly supported by very recent and detailed studies with a bovine model by Woclawek-Potocka and Yamamoto’s groups [ 29 , 37 ]. They demonstrated expression of mRNAs for ATX, group IVA PLA 2 , and PLA 1 α in epithelial and stromal cells and LPA content in oviductal tissues.…”

“…However, ATX/LPA system in post-ovulation processes such as the dynamics of released COCs and fertilizing and dividing potential within the oviduct remains to be clarified. Very recent studies with a bovine model demonstrated mRNA expression of synthetic enzymes (ATX, Group IVA PLA 2 , and PLA 1 α) and signaling receptors (all LPA receptors except for LPA 5 ) in the oviduct [ 29 , 37 ]. Given these findings, we here focus on 1) expression of enzyme and receptor proteins in ovulated COCs and the oviduct and 2) possible in vivo functional role(s) of ATX/LPA system in COCs around fertilization in a rat model.…”

Differential expression pattern of genes involved in oxygen metabolism in epithelial oviductal cells during primary in vitro culture

“…However, it reaches the highest level of expression in the brain [14]. Abovine study also revealed its presence in oviductal epithelial cells and the role of LPA in fertilization, as well as in gamete and embryo transport through an influence on prostaglandin production [15].…”

Cytidinediphosphate diacylglycerol synthase—Mediated phosphatidic acid metabolism is crucial for early embryonic development of Arabidopsis

“…Recent studies revealed the crucial role of PA in embryonic development of animals. Locally produced lysophosphatidic acid (LPA, an intermediate of PA pathway), stimulates PG production and regulates the oviductal functions, and further participates in fertilization and transport of gametes and embryo [ 18 ]. In addition, phospholipase D1 (PLD1)-produced PA activates mTORC1 kinase activity, which in turn promotes decidualization and the successful implantation of embryo [ 19 ].…”