Local heat-shock priming-induced improvement in microvascular perfusion in osteomyocutaneous flaps is mediated by heat-shock protein 32

Rücker, Martin; Schäfer, T.; Roesken, Frank; Spitzer, W.J.; Bauer, Michael; Menger, Michael D.

doi:10.1046/j.1365-2168.2001.01682.x

Cited by 35 publications

(28 citation statements)

References 21 publications

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“…25 Previous studies have demonstrated that heat shock increases capillary perfusion in flap tissue. 17 Thus, the increased baseline CBF may have contributed to the accelerated recanalization. However, although baseline skin CBF did not differ significantly between heat shock preconditioning versus non-preconditioned controls, heat shock significantly accelerated recanalization, similarly as observed in the other tissues, in which baseline CBF was different between the 2 groups.…”

Section: Rü Cker Et Al Microvascular Recanalization By Heat Shock Primentioning

confidence: 99%

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Local Heat Shock Priming Promotes Recanalization of Thromboembolized Microvasculature by Upregulation of Plasminogen Activators

Rücker

Schäfer²,

Scheuer³

et al. 2006

ATVB

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Objective-Thromboembolization and subsequent microvascular perfusion failure is implicated in the pathology of a variety of diseases, including transient ischemic attack (TIA), stroke, and myocardial infarction, and also for the complications after interventional and microsurgical procedures in coronary heart disease and peripheral arterial occlusive disease. In vitro heat shock priming has been suggested to induce plasminogen activators, which are the major upregulators of the fibrinolytic system. Herein, we determined whether local heat shock priming endogenously upregulates plasminogen activators also in vivo, and whether this promotes recanalization of thromboembolized microvasculature. Methods and Results-To induce thromboembolization, a suspension of preformed microthrombi (maximum diameter: 40 m) was injected via the femoral artery into the left hindlimbs of anesthetized rats. Local heat shock priming (42.5°C, 30 minutes) was performed 24 hours before embolization and resulted in a significant increase of endothelium-derived plasminogen activator expression. The study of the microcirculation by intravital microscopy revealed in all tissues analyzed (muscle, periosteum, subcutis, and skin) that heat shock priming significantly (PϽ0.05) accelerates recanalization of the thromboembolized microvasculature when compared with nonprimed and sham-primed controls. Importantly, the addition of plasminogen activator inhibitor-1 to the microthrombi suspension completely blunted the heat shock-induced acceleration of microvascular recanalization. Key Words: heat shock Ⅲ intravital microscopy Ⅲ microcirculation Ⅲ plasminogen activator inhibitor-1 Ⅲ thromboembolization Ⅲ urokinase plasminogen activator Conclusions-Heat

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Section: Rü Cker Et Al Microvascular Recanalization By Heat Shock Primentioning

confidence: 99%

“…17 During local heating, muscle temperature was increased to 42.5°C and was kept constant for 30 minutes. Animals undergoing a sham heating procedure were also anesthetized 24 hours before thromboembolization, and the hindlimb was exposed in a waterbath to 30°C for 30 minutes.…”

Section: Stress Conditioningmentioning

confidence: 99%

Local Heat Shock Priming Promotes Recanalization of Thromboembolized Microvasculature by Upregulation of Plasminogen Activators

Rücker

Schäfer²,

Scheuer³

et al. 2006

ATVB

View full text Add to dashboard Cite

show abstract

“…Epi-illumination fluorescence microscopy was performed with a modified Zeiss Axiotech microscope (Zeiss, Jena, Germany) with a 100-W mercury arc-lamp [9]. The microscope included a blue filter combination (450-490 nm excitation/>520 nm emission wavelength) for imaging fluorescence [9].…”

Section: Intravital Fluorescence Microscopymentioning

confidence: 99%

Protective skeletal muscle arteriolar vasomotion during critical perfusion conditions of osteomyocutaneous flaps is not mediated by nitric oxide and endothelins

Rücker

Strobel

Vollmar

et al. 2003

Langenbeck's Archives of Surgery

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“…Some studies have reported that exposing soft tissue to a supraphysiologic stress (mechanical, thermal, or chemical) could induce a cytoprotective effect [8][9][10][11]. Following such exposure, cells were able to withstand a subsequent metabolic insult that would have otherwise been lethal.…”

Section: Introductionmentioning

confidence: 99%

Laser preconditioning of calvarial bone prior to an X‐ray radiation injury: A preliminary in vivo study of the vascular response

et al. 2008

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Background and Objectives: Thermal preconditioning prior to injury induces a cytoprotective effect on soft tissues and promotes their recovery. Lasers are an adequate tool to generate controlled and reproducible heat. X-ray irradiation induces a chronic antiangiogenic effect on bone, affecting its healing and remodeling processes. The aim of this study was to investigate the effect of laser preconditioning on the re-vascularization of X-ray irradiated bone. Materials and Methods: A bone chamber was implanted onto the calvaria of rabbits to study the vascularization process. Digital pictures were taken of the vascular plexus at the target bone site using a modified digital camera. Vascular density (VD) was determined using image processing. It was defined as the ratio of blood vessel pixels to the total number of pixels to the region of interest. Laser preconditioning was performed with a diode laser (810 nm, 2 W, 3 seconds, 48 J/cm 2 , 4 mm). A 12-week follow-up study was performed on 20 rabbits divided into four groups: #1: control group (n ¼ 5); #2: laser irradiation alone (n ¼ 5). #3: X-ray radiation (18.75 Gy) alone (n ¼ 5), #4: laser preconditioning 24 hours prior to X-ray radiation (n ¼ 5). Results: VD remained stable during the 12-week follow up for group #1. No significant difference was observed between laser irradiation group (#2) and control group (#1) (P > 0.5). The angiolytic action of X-ray radiation was confirmed in groups #3 and #4, which were statistically different from group #1 (P<0.001). However, the decrease of the vascularization was limited in group #4 highlighting a different evolution between group #3 and #4 (P<0.05). These results were confirmed by histological analysis. Discussion and Conclusion: The bone chamber is an effective reproducible method for the longitudinal analysis of the dynamics of vascularization. Our findings have shown that laser preconditioning is capable of preserving vascularization in an X-ray irradiated bone site, thus suggesting a novel approach for promoting the healing of bone tissue in which the vascular supply has been damaged.

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Local heat-shock priming-induced improvement in microvascular perfusion in osteomyocutaneous flaps is mediated by heat-shock protein 32

Abstract: The present study demonstrated that stress conditioning by local heat-shock priming improves nutritive perfusion in osteomyocutaneous flaps by capillary dilatation, probably mediated through the vasoactive action of HSP-32.

Cited by 35 publications

References 21 publications

Local Heat Shock Priming Promotes Recanalization of Thromboembolized Microvasculature by Upregulation of Plasminogen Activators

Local Heat Shock Priming Promotes Recanalization of Thromboembolized Microvasculature by Upregulation of Plasminogen Activators

Protective skeletal muscle arteriolar vasomotion during critical perfusion conditions of osteomyocutaneous flaps is not mediated by nitric oxide and endothelins

Laser preconditioning of calvarial bone prior to an X‐ray radiation injury: A preliminary in vivo study of the vascular response

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