Local mechanical properties of bladder cancer cells measured by AFM as a signature of metastatic potential

Abidine, Yara; Laurent, V.; Michel, Richard; Duperray, Alain; Verdier, Claude

doi:10.1140/epjp/i2015-15202-6

Cited by 43 publications

(63 citation statements)

References 52 publications

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“…In our study, by using AFM to analyze cell membrane biomechanical properties, we found that cell elasticity was significantly altered with the treatment of 30μM Y-27632 as the Young's modulus of treatment group showing significant upregulation. Consistently, Abidine et al ., also found that Y-27632 increased the plateau modulus of bladder cancer cell [ 46 ]. Recently, growing evidence suggests that the mechanical properties of the cell are closely related to cell proliferation, apoptosis, migration and adhesion.…”

Section: Discussionmentioning

confidence: 99%

ROCK Inhibitor Y-27632 Promotes Human Retinal Pigment Epithelium Survival by Altering Cellular Biomechanical Properties

Qin

Fang

et al. 2018

CMM

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Purpose: Dysfunction or death of retinal pigment epithelial (RPE) cells is a common pathogenesis of various types of retinal degenerative diseases. Recent reports indicated that ROCK pathway inhibitors regulate cell proliferation or apoptosis in a cell-type-dependent manner. Here, we aim to investigate the effect of ROCK inhibitor Y-27632 on the human retinal pigment epithelium (RPE) in vitro.Methods: Cell proliferation and apoptosis were analyzed by CCK-8 and flow cytometry respectively. Cell proliferation markers were detected by immunofluorescence and western blot. Cell morphology was evaluated using scanning electron microscopy. The topography and biomechanical properties of living cells were assessed using atomic force microscope (AFM). In addition, cytoskeleton and epithelial-mesenchymal transition (EMT) markers were detected by western blot and immunofluorescence.Results: 30μM Y-27632 significantly promoted cell proliferation and decreased apoptosis. Compared with control group, human retinal pigment epithelial cell line ARPE-19 cells treated with 30μM Y-27632 exhibited significantly decreased cytomembrane roughness (Ra: 41.04±1.63nm vs. 24.41±0.75nm, P<0.01; Rq: 51.56±2.03nm vs. 30.81±0.95nm, P<0.01) and increased elasticity modulus (16.66±0.83KPa vs. 32.55±1.48KPa, P<0.01). In addition, the inhibition of ROCK activity by Y-27632 caused cell elongation and reorganization of microfilaments and microtubules of cytoskeletons.Conclusion: Taken together, our data demonstrated that Y-27632 could alter biomechanical properties and reorganized cytoskeletons to promote RPE cell survival. These results are an important step toward the future application of Y-27632 in retinal degenerative diseases.

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Section: Discussionmentioning

confidence: 99%

ROCK Inhibitor Y-27632 Promotes Human Retinal Pigment Epithelium Survival by Altering Cellular Biomechanical Properties

Qin

Fang

et al. 2018

CMM

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“…Li et al, 2009;Mandal et al, 2016). Fibroblasts (Thoumine & Ott, 1997a;Wottawah et al, 2005;Park et al, 2005), hepatoma cell lines (Zhang et al, 2002), prostate cancer cells (Faria et al, 2008), kidney cell lines (Rebelo et al, 2013), glioma cells (Pogoda et al, 2014) and bladder cancer cells (Bertseva et al, 2012;Lekka et al, 1999;Abidine et al, 2015) have also been studied. Cell lines from patients with primary oral or thyroid cancer were used for the first time to our knowledge by Remmerbach et al (2009) and Prabhune et al (2012), respectively.…”

Section: Mechanics Of Normal Cells Versus Cancer Cells From the Same mentioning

confidence: 99%

Are cancer cells really softer than normal cells?

Charlotte

Goud

Manneville

2017

Biology of the Cell

295

236

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Solid tumours are often first diagnosed by palpation, suggesting that the tumour is more rigid than its surrounding environment. Paradoxically, individual cancer cells appear to be softer than their healthy counterparts. In this review, we first list the physiological reasons indicating that cancer cells may be more deformable than normal cells. Next, we describe the biophysical tools that have been developed in recent years to characterise and model cancer cell mechanics. By reviewing the experimental studies that compared the mechanics of individual normal and cancer cells, we argue that cancer cells can indeed be considered as softer than normal cells. We then focus on the intracellular elements that could be responsible for the softening of cancer cells. Finally, we ask whether the mechanical differences between normal and cancer cells can be used as diagnostic or prognostic markers of cancer progression.

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“…Liu et al, reported that bladder cancer cell at higher grades possess larger SMC values and lower Young's modulus than cancer cells at lower grades, by using two independent techniques of microfluidic and atomic force microscope (AFM) to measure RT4 and T24 cells [28]. Abidine et al, also observed that cellular elastic modulus is associated with cells' invasion ability by measuring the elastic modulus of RT112, T24, and J82 cells using AFM [29]. However, these studies only measured either mechanical or electrical properties of cells due to the limits of the adopted tools.…”

Section: Introductionmentioning

confidence: 99%

Simultaneously Quantifying Both Young’s Modulus and Specific Membrane Capacitance of Bladder Cancer Cells with Different Metastatic Potential

et al. 2020

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Both Young’s modulus and specific membrane capacitance (SMC) are two important physical parameters for characterizing cell status. In this paper, we utilized a thin-neck-micropipette aspiration system to simultaneously quantify Young’s modulus and SMC value of six types of cell lines in different progression grades, which include four grades from the lowest metastatic potential G1 to the highest potential G4. We investigated how these two physical properties possess heterogeneities in bladder cancer cells with different grades and what roles they might play in grading bladder cancer. The characterization results of these cells of different cancer grades is linearly correlated with the cancer grades, showing that the Young’s modulus is negatively linearly correlated with bladder cancer grades, while SMC shows a positive linear correlation. Furthermore, the combination of these two physical properties on a scatter diagram clearly shows the cell groups with different cancer grades, which means that this combination could be a potential tumor grading marker to identify cancer cells with different metastatic potential.

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Local mechanical properties of bladder cancer cells measured by AFM as a signature of metastatic potential

Cited by 43 publications

References 52 publications

ROCK Inhibitor Y-27632 Promotes Human Retinal Pigment Epithelium Survival by Altering Cellular Biomechanical Properties

ROCK Inhibitor Y-27632 Promotes Human Retinal Pigment Epithelium Survival by Altering Cellular Biomechanical Properties

Are cancer cells really softer than normal cells?

Simultaneously Quantifying Both Young’s Modulus and Specific Membrane Capacitance of Bladder Cancer Cells with Different Metastatic Potential

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